Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/29570
Title: Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
Authors: SANTOS, Anderson Pacheco dosCARVALHO, Maria Esther deMEIRELLES, Luciana ReginaANDRADE JUNIOR, Heitor Franco de
Citation: REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, v.51, n.5, p.665-669, 2018
Abstract: Introduction: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. Methods: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. Results: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. Conclusions: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
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Artigos e Materiais de Revistas Científicas - FM/MPT
Departamento de Patologia - FM/MPT

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/49
LIM/49 - Laboratório de Protozoologia


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