Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/29721
Title: Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)
Authors: CASTRO, TalitaSABALZA, MaiteBARBER, CherylABRAMS, WilliamCOSTA, Antonio Charlys DaMILAGRES, Flavio Augusto De PaduaBRAZ-SILVA, Paulo HenriqueMALAMUD, DanielGALLOTTINI, Marina
Citation: JOURNAL OF ORAL MICROBIOLOGY, v.10, n.1, article ID 1510712, 6p, 2018
Abstract: Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8min. Saliva detected more samples (p=0.0042) and had faster Tp (p=0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/46
LIM/46 - Laboratório de Parasitologia Médica

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar


Files in This Item:
File Description SizeFormat 
art_CASTRO_Rapid_diagnosis_of_Zika_virus_through_saliva_and_2018.PDFpublishedVersion (English)1.44 MBAdobe PDFThumbnail
View/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.