Effects of ketamine/xylazine and isoflurane on rat brain glucose metabolism measured by F-18-fluorodeoxyglucose-positron emission tomography

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art_PRANDO_Effects_of_ketaminexylazine_and_isoflurane_on_rat_brain_2019.PDF (519.38 KB)
Citações na Scopus
12
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Autores
Citação
EUROPEAN JOURNAL OF NEUROSCIENCE, v.49, n.1, p.51-61, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The aim of the present study was to investigate changes in glucose metabolism in male Wistar rats induced by the anesthetics isoflurane and ketamine combined with xylazine via F-18-fluorodeoxyglucose-positron emission tomography. We analyzed the differential effects of the anesthetics on F-18-fluorodeoxyglucose uptake and pharmacokinetics in 33 rats using quantification methods: (a) the standardized uptake value, (b) voxel-based analyses, and (c) kinetic analysis. Both anesthetics reduced glucose uptake in the entire brain. The voxel-based analyses detected smaller uptake reductions in the bilateral primary somatosensory system cortex and part of the limbic system in the ketamine-xylazine (KX) group and in the vestibular nucleus in the isoflurane group. Through kinetic analysis, we found that the volume of distribution and the membrane transport rate K-1 were reduced in the KX group. Through various methods of F-18-fluorodeoxyglucose-positron emission tomography quantification, the present study found that anesthesia with the ketamine-xylazine combination induced a global reduction of glucose metabolism compared with isoflurane; this reduction of metabolism was relatively lower in the primary somatosensory cortex and part of the limbic system. The volume of distribution of F-18-fluorodeoxyglucose and its Glut1-mediated transport across the brain membranes (K-1) were decreased in the KX group.
Palavras-chave
anesthetics, brain energy metabolism, F-18-Fluorodeoxyglucose, positron emission tomography, Wistar
URI
https://observatorio.fm.usp.br/handle/OPI/30829
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MDR
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/InRad
Artigos e Materiais de Revistas Científicas - LIM/08
Artigos e Materiais de Revistas Científicas - LIM/21
Artigos e Materiais de Revistas Científicas - LIM/43