Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author SIQUEIRA, Lumena P. Machado FMUSP-HC
GIMENES, Viviane M. Favero FMUSP-HC
FREITAS, Roseli Santos de FMUSP-HC
MELHEM, Marcia de Souza Carvalho
BONFIETTI, Lucas Xavier
JR, Afonso Rafael da Silva FMUSP-HC
SANTOS, Leticia B. Souza FMUSP-HC
MOTTA, Adriana L. FMUSP-HC
ROSSI, Flavia FMUSP-HC
BENARD, Gil FMUSP-HC
JR, Joao N. de Almeida FMUSP-HC
dc.date.issued 2019
dc.identifier.citation JOURNAL OF CLINICAL MICROBIOLOGY, v.57, n.1, article ID UNSP e01282, 7p, 2019
dc.identifier.issn 0095-1137
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/30836
dc.description.abstract Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for C. neoformans and C. gattii genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different C. neoformans (VNI, VNII, VNIII, and VNIV) and C. gattii (VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMerieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all C. neoformans and C. gattii genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.
dc.description.sponsorship · National Council of Technological and Scientific Development [CNPQ 421374/2016-0]
dc.language.iso eng
dc.publisher AMER SOC MICROBIOLOGY
dc.relation.ispartof Journal of Clinical Microbiology
dc.rights restrictedAccess
dc.subject Cryptococcus gattii; Cryptococcus neoformans; MALDI-TOF; VITEK MS; genotypic identification
dc.subject.other desorption ionization-time; flight mass-spectrometry; molecular types; identification; complex; ivd
dc.title Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes
dc.type article
dc.rights.holder Copyright AMER SOC MICROBIOLOGY
dc.description.group LIM/53
dc.description.group LIM/03
dc.description.group LIM/56
dc.identifier.doi 10.1128/JCM.01282-18
dc.identifier.pmid 30429250
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author SIQUEIRA, Lumena P. Machado:HC:LIM/53
hcfmusp.author GIMENES, Viviane M. Favero:HC:ICHC
hcfmusp.author FREITAS, Roseli Santos de:HC:LIM/53
hcfmusp.author JR, Afonso Rafael da Silva:HC:ICHC
hcfmusp.author SANTOS, Leticia B. Souza:FM:
hcfmusp.author MOTTA, Adriana L.:HC:ICHC
hcfmusp.author ROSSI, Flavia:HC:ICHC
hcfmusp.author BENARD, Gil:FM:MDT
hcfmusp.author JR, Joao N. de Almeida:HC:ICHC
hcfmusp.author.external · MELHEM, Marcia de Souza Carvalho:Adolfo Lutz Inst, Secao Micol, Div Tecn Biol Med, Sao Paulo, Brazil
· BONFIETTI, Lucas Xavier:Adolfo Lutz Inst, Secao Micol, Div Tecn Biol Med, Sao Paulo, Brazil
hcfmusp.origem.id WOS:000455018000014
hcfmusp.origem.id 2-s2.0-85059373523
hcfmusp.publisher.city WASHINGTON
hcfmusp.publisher.country USA
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dc.description.index MEDLINE
dc.identifier.eissn 1098-660X
hcfmusp.citation.scopus 1
hcfmusp.citation.wos 0
hcfmusp.affiliation.country Brasil


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