Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion

Imagem de Miniatura
Arquivos
art_CASTRO-E-SILVA_Evaluation_of_the_therapeutic_effect_of_methylene_blue_2018.PDF (538.3 KB)
Citações na Scopus
3
Tipo de produção
article
Data de publicação
2018
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ACTA CIRURGICA BRASILEIRA
Autores
SILVEIRA, Marina Rodrigues Garcia
ZORZI, Patricia
LIU, Jordan Bistafa
CAMPOS, Daniel Tofoli Queiroz
VICTORINO, Joao Paulo
JORDANI, Maria Cecilia
MENDES, Karina Dal Sasso
EVORA, Paulo Roberto Barbosa
Citação
ACTA CIRURGICA BRASILEIRA, v.33, n.12, p.1043-1051, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Purpose: To analyze the effect of methylene blue (MB) therapy during the liver ischemia-reperfusion injury (I/R) process. Methods: Thirty-five male Wistar rats were used, (70%) submitted to partial ischemia (IR) or not (NIR) (30%) were obtained from the same animal. These animals were divided into six groups: 1) Sham (SH), 2) Sham with MB (SH-MB); 3) I/R, submitted to 60 minutes of partial ischemia and 15 minutes of reperfusion; 4) NI/R, without I/R obtained from the same animal of group I/R; 5) I/R-MB submitted to I/R and MB and 6) NI/R-MB, without I/R. Mitochondria) function was evaluated. Osmotic swelling of mitochondria as well as the determination of malondialdehyde (MDA) was evaluated. Serum (ALT/AST) dosages were also performed. MB was used at the concentration of 15mg/kg, 15 minutes before hepatic reperfusion. Statistical analysis was done by the Mann Whitney test at 5%. Results: State 3 shows inhibition in all ischemic groups. State 4 was increased in all groups, except the I/R-MB and NI/R-MB groups. RCR showed a decrease in all I/R and NI/R groups. Mitochondria) osmotic swelling showed an increase in all I/R NI/R groups in the presence or absence of MB. About MDA, there was a decrease in SH values in the presence of MB and this decrease was maintained in the I/R group. AST levels were increased in all ischemic with or without MB. Conclusions: The methylene blue was not able to restore the mitochondria) parameters studied. Also, it was able to decrease lipid peroxidation, preventing the formation of reactive oxygen species.
Palavras-chave
Ischemia, Reperfusion, Methylene Blue, Mitochondria, Liver, Rats
URI
https://observatorio.fm.usp.br/handle/OPI/30849
Referências
  1. Evora PRB, 2016, ACTA CIR BRAS, V31, P2, DOI 10.1590/S0102-86502016001300001
  2. BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  3. Casillas-Ramirez A, 2006, LIFE SCI, V79, P1881, DOI 10.1016/j.lfs.2006.06.024
  4. CHANCE B, 1956, ADV ENZYMOL REL S BI, V17, P65
  5. Collange O, 2013, EUR J VASC ENDOVASC, V45, P168, DOI 10.1016/j.ejvs.2012.11.011
  6. de Almeida TN, 2018, TRANSPL P, V50, P841, DOI 10.1016/j.transproceed.2018.02.002
  7. Di Y, 2015, MOL MED, V21, P420, DOI 10.2119/molmed.2015.00038
  8. Diaz-Juarez J, 2006, SCAND J GASTROENTERO, V41, P223, DOI 10.1080/00365520510024142
  9. Fiegel M, 2012, SEMIN CARDIOTHORAC V, V16, P106, DOI 10.1177/1089253212444791
  10. Fondevila C, 2010, LIVER TRANSPLANT, V16, P364, DOI 10.1002/lt.21989
  11. Fukazawa K, 2011, J HEPATO-BIL-PAN SCI, V18, P406, DOI 10.1007/s00534-010-0344-7
  12. Giannini EG, 2005, CAN MED ASSOC J, V172, P367, DOI 10.1503/cmaj.1040752
  13. Glantzounis GK, 2005, LIVER TRANSPLANT, V11, P1031, DOI 10.1002/lt.20504
  14. Guan LY, 2014, WORLD J GASTRO SURG, V6, P122, DOI 10.4240/wjgs.v6.i7.122
  15. Handy DE, 2012, ANTIOXID REDOX SIGN, V16, P1323, DOI 10.1089/ars.2011.4123
  16. HENRY RJ, 1960, AM J CLIN PATHOL, V34, P381
  17. Knobel E, 1996, Arq Bras Cardiol, V67, P263
  18. Koelzow H, 2002, ANESTH ANALG, V94, P824, DOI 10.1097/00000539-200204000-00009
  19. Lapenna D, 2001, FREE RADICAL BIO MED, V31, P331, DOI 10.1016/S0891-5849(01)00584-6
  20. Li J, 2015, EUR REV MED PHARMACO, V19, P2036
  21. Miranda Luiz Eduardo C., 2004, Acta Cir. Bras., V19, P1, DOI 10.1590/S0102-86502004000100001
  22. Pedersen PL, 1978, METHOD CELL BIOL, V20, P411
  23. Rosique RG, 2011, TRANSPL P, V43, P3643, DOI 10.1016/j.transproceed.2011.08.108
  24. SALARIS SC, 1991, BIOCHEM PHARMACOL, V42, P499, DOI 10.1016/0006-2952(91)90311-R
  25. Siniscalchi A, 2016, WORLD J GASTROENTERO, V22, P1551, DOI 10.3748/wjg.v22.i4.1551
  26. Vilalva KH, 2018, CLINICS, V73, DOI 10.6061/clinics/2018/e113
  27. Visarius TM, 1997, FEBS LETT, V412, P157, DOI 10.1016/S0014-5793(97)00767-9
  28. ZORATTI M, 1995, BBA-REV BIOMEMBRANES, V1241, P139, DOI 10.1016/0304-4157(95)00003-A

Coleções
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/37