Bi-allelic Variants in TONSL Cause SPONASTRIME Dysplasia and a Spectrum of Skeletal Dysplasia Phenotypes

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art_BURRAGE_Biallelic_Variants_in_TONSL_Cause_SPONASTRIME_Dysplasia_and_2019.PDF (1.96 MB)
Citações na Scopus
23
Tipo de produção
article
Data de publicação
2019
Editora
CELL PRESS
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
BURRAGE, Lindsay C.
REYNOLDS, John J.
BARATANG, Nissan Vida
PHILLIPS, Jennifer B.
WEGNER, Jeremy
MCFARQUHAR, Ashley
HIGGS, Martin R.
CHRISTIANSEN, Audrey E.
LANZA, Denise G.
SEAVITT, John R.
Autor de Grupo de pesquisa
Univ Washington Ctr Mendelian
Undiagnosed Dis Network
Editores
Coordenadores
Organizadores
Citação
AMERICAN JOURNAL OF HUMAN GENETICS, v.104, n.3, p.422-438, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
SPONASTRIME dysplasia is an autosomal-recessive spondyloepimetaphyseal dysplasia characterized by spine (spondylar) abnormalities, midface hypoplasia with a depressed nasal bridge, metaphyseal striations, and disproportionate short stature. Scoliosis, coxa vara, childhood cataracts, short dental roots, and hypogammaglobulinemia have also been reported in this disorder. Although an autosomal-recessive inheritance pattern has been hypothesized, pathogenic variants in a specific gene have not been discovered in individuals with SPONASTRIME dysplasia. Here, we identified bi-allelic variants in TONSL, which encodes the Tonsoku-like DNA repair protein, in nine subjects (from eight families) with SPONASTRIME dysplasia, and four subjects (from three families) with short stature of varied severity and spondylometaphyseal dysplasia with or without immunologic and hematologic abnormalities, but no definitive metaphyseal striations at diagnosis. The finding of early embryonic lethality in a Tonsl(-/-) murine model and the discovery of reduced length, spinal abnormalities, reduced numbers of neutrophils, and early lethality in a tonsl(-/-) zebrafish model both support the hypomorphic nature of the identified TONSL variants. Moreover, functional studies revealed increased amounts of spontaneous replication fork stalling and chromosomal aberrations, as well as fewer camptothecin (CPT)-induced RAD51 foci in subject-derived cell lines. Importantly, these cellular defects were rescued upon re-expression of wild-type (WT) TONSL; this rescue is consistent with the hypothesis that hypomorphic TONSL variants are pathogenic. Overall, our studies in humans, mice, zebrafish, and subject-derived cell lines confirm that pathogenic variants in TONSL impair DNA replication and homologous recombination-dependent repair processes, and they lead to a spectrum of skeletal dysplasia phenotypes with numerous extra-skeletal manifestations.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/31941
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICr
Artigos e Materiais de Revistas Científicas - LIM/36