Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules

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art_FIGO_Cashew_Tree_Pollen_An_Unknown_Source_of_IgEReactive_2019.PDF (1.72 MB)
Citações na Scopus
6
Tipo de produção
article
Data de publicação
2019
Editora
MDPI
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
AQUINO, Denise Neiva Santos de
POMIECINSKI, Fabiane
GADERMAIER, Gabriele
BRIZA, Peter
AMARAL, Jonatas Bussador do
MARTINS, Carlo de Oliveira
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.20, n.10, article ID 2397, 15p, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Pollinosis is sub-diagnosed and rarely studied in tropical countries. Cashew tree pollen has been reported as an allergen source although the knowledge of its immunoglobulin E (IgE)-reactive molecules is lacking. Therefore, this work aimed to identify IgE-reactive molecules and provide a proteomic profile of this pollen. From the 830 proteins identified by shotgun analysis, 163 were annotated to gene ontology, and a list of 39 proteins filtered for high confidence was submitted to the Allfam database where nine were assigned to allergenic families. Thus, 12 patients from the northeast of Brazil with persistent allergic rhinitis and aggravation of symptoms during cashew flowering season were selected. Using a 2D-based approach, we identified 20 IgE-reactive proteins, four already recognized as allergens, including a homolog of the birch isoflavone-reductase (Bet v 6). IgE-reactivity against the extract in native form was confirmed for five patients in ELISA, with three being positive for Bet v 6. Herein, we present a group of patients with rhinitis exposed to cashew tree pollen with the first description of IgE-binding proteins and a proteomic profile of the whole pollen. Cashew tree pollen is considered an important trigger of rhinitis symptoms in clinical practice in the northeast of Brazil, and the elucidation of its allergenic molecules can improve the diagnostics and treatment for allergic patients.
Palavras-chave
novel allergens, Brazil, pollinosis, proteome, shotgun analysis, aeroallergens
URI
https://observatorio.fm.usp.br/handle/OPI/32998
Referências
  1. ACR Institute for Tropical and Subtropical Crops SA, 2015, CULT CASH NUTS
  2. Aronne G, 2001, BIOTECH HISTOCHEM, V76, P35, DOI 10.1080/714028122
  3. ASBAI, 2009, ASS BRAS AL IM INF P
  4. BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  5. Buters JTM, 2012, ATMOS ENVIRON, V55, P496, DOI 10.1016/j.atmosenv.2012.01.054
  6. Candiano G, 2004, ELECTROPHORESIS, V25, P1327, DOI 10.1002/elps.200305844
  7. Carini A., 1908, REV MED SAO PAULO, V11, P23
  8. Carpentier SC, 2005, PROTEOMICS, V5, P2497, DOI 10.1002/pmic.200401222
  9. CashewInfo. com, 2018, CASHEWINFO
  10. Comstock SS, 2006, J ALLERGY CLIN IMMUN, V117, pS49, DOI 10.1016/j.jaci.2005.12.202
  11. D'Amato G, 2007, ALLERGY, V62, P976, DOI 10.1111/j.1398-9995.2007.01393.x
  12. DRIESSEN MNBM, 1991, EUR RESPIR J, V4, P359
  13. Dutra B.M.R.S., 2001, REV BRAS ALERG IMUNO, V24, P89
  14. EAACI, 2016, MOL ALL US GUID
  15. Embrapa, CULT CAJ 2003
  16. FERNANDES L, 1995, J ALLERGY CLIN IMMUN, V95, P501, DOI 10.1016/S0091-6749(95)70311-X
  17. Flinterman AE, 2008, CURR OPIN ALLERGY CL, V8, P261, DOI 10.1097/ACI.0b013e3282ffb145
  18. Fonseca AE, 2002, CROP SCI, V42, P2201, DOI 10.2135/cropsci2002.2201
  19. Galan C, 2013, ALLERGY, V68, P809, DOI 10.1111/all.12144
  20. Gruehn S, 2003, INT ARCH ALLERGY IMM, V131, P91, DOI 10.1159/000070924
  21. Huecas S, 2001, J BIOL CHEM, V276, P27959, DOI 10.1074/jbc.M103041200
  22. Jutel M, 2015, J ALLERGY CLIN IMMUN, V136, P556, DOI 10.1016/j.jaci.2015.04.047
  23. Karamloo F, 1999, J ALLERGY CLIN IMMUN, V104, P991, DOI 10.1016/S0091-6749(99)70080-1
  24. Matricardi PM, 2019, J ALLERGY CLIN IMMUN, V143, P831, DOI 10.1016/j.jaci.2018.12.1021
  25. McCrone W.C., 1973, PARTICLE ATLAS
  26. Menezes EA, 2002, J INVEST ALLERG CLIN, V12, P25
  27. Mitsumoto K, 2009, J BIOSCI BIOENG, V107, P90, DOI 10.1016/j.jbiosc.2008.10.001
  28. Pablos I, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-06213-z
  29. PINNICK RG, 1995, AEROSOL SCI TECH, V23, P653, DOI 10.1080/02786829508965345
  30. Postigo I, 2009, J INVEST ALLERG CLIN, V19, P504
  31. Quiralte J, 2007, J INVEST ALLERG CLIN, V17, P24
  32. Radauer C, 2008, J ALLERGY CLIN IMMUN, V121, P847, DOI 10.1016/j.jaci.2008.01.025
  33. Radev Z., 2018, AM J ENTOMOL, V2, P23, DOI [10.11648/j.aje.20180203.11, DOI 10.11648/J.AJE.20180203.11]
  34. Reitsma M, 2016, J AGR FOOD CHEM, V64, P1191, DOI 10.1021/acs.jafc.5b04401
  35. Roberts G, 2013, ALLERGY, V68, P1102, DOI 10.1111/all.12235
  36. Ronneberger Olaf, 2002, Aerobiologia, V18, P107, DOI 10.1023/A:1020623724584
  37. Rosario Filho N.A., 1987, REV BRAS ALERGIA IMU, V10, P25
  38. Saha B, 2017, J PROTEOMICS, V165, P1, DOI 10.1016/j.jprot.2017.05.021
  39. Taketomi Ernesto Akio, 2006, Rev. Bras. Otorrinolaringol., V72, P562, DOI 10.1590/S0034-72992006000400020
  40. Valenta R, 2011, ALLERGY, V66, P775, DOI 10.1111/j.1398-9995.2011.02565.x
  41. Vejvar E., 2011, NOVEL ALLERGEN UNPUB
  42. Vieths S, 1998, SCAND J IMMUNOL, V47, P263

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/19
Artigos e Materiais de Revistas Científicas - LIM/60