Macrophages From Subjects With Isolated GH/IGF-I Deficiency Due to a GHRH Receptor Gene Mutation Are Less Prone to Infection by Leishmania amazonensis

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art_BARRIOS_Macrophages_From_Subjects_With_Isolated_GHIGFI_Deficiency_Due_2019.PDF (687.8 KB)
Citações na Scopus
10
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
Título da Revista
ISSN da Revista
Título do Volume
Editora
FRONTIERS MEDIA SA
Autores
BARRIOS, Monica R.
CAMPOS, Viviane C.
PERES, Nalu T. A.
OLIVEIRA, Lais L. de
CAZZANIGA, Rodrigo A.
SANTOS, Marcio B.
AIRES, Murilo B.
SILVA, Ricardo L. L.
BARRETO, Aline
Citação
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v.9, article ID 311, 7p, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Isolated growth hormone (GH) deficiency (IGHD) affects approximately 1 in 4,000 to 1 in 10,000 individuals worldwide. We have previously described a large cohort of subjects with IGHD due to a homozygous mutation in the GH releasing hormone (GHRH) receptor gene. These subjects exhibit throughout the life very low levels of GH and its principal mediator, the Insulin Growth Factor-I (IGF-I). The facilitating role of IGF-I in the infection of mouse macrophages by different Leishmania strains is well-known. Nevertheless, the role of IGF-I in Leishmania infection of human macrophages has not been studied. This study aimed to evaluate the behavior of Leishmania infection in vitro in macrophages from untreated IGHD subjects. To this end, blood samples were collected from 14 IGHD individuals and 14 age and sex-matched healthy controls. Monocytes were isolated and derived into macrophages and infected with a strain of Leishmania amazonensis. In addition, IGF-I was added to culture medium to evaluate its effect on the infection. Cytokines were measured in the culture supernatants. We found that macrophages from IGHD subjects were less prone to Leishmania infection compared to GH sufficient controls. Both inflammatory and anti-inflammatory cytokines increase only in the supernatants of the control macrophages. Addition of IGF-I to the culture medium increased infection rates. In conclusion, we demonstrated that IGF-I is crucial for Leishmania infection of human macrophages.
Palavras-chave
growth hormone deficiency, insulin growth factor-I deficiency, macrophages, leishmaniasis, phagocytosis, infection, immunology
URI
https://observatorio.fm.usp.br/handle/OPI/33546
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - LIM/38