Effects of the serine protease inhibitor rBmTI-A in an experimental mouse model of chronic allergic pulmonary inflammation

Imagem de Miniatura
Arquivos
art_FLORENCIO_Effects_of_the_serine_protease_inhibitor_rBmTIA_in_2019.PDF (2.31 MB)
Citações na Scopus
7
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
NATURE PUBLISHING GROUP
Autores
ALMEIDA, Robson S. de
DURAN, Adriana F.
SASAKI, Sergio D.
Citação
SCIENTIFIC REPORTS, v.9, article ID 12624, 13p, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
To evaluate whether a recombinant serine protease inhibitor (rBmTI-A) modulates inflammation in an experimental model of chronic allergic lung inflammation. Balb/c mice were divided into four groups: SAL (saline), OVA (sensitized with ovalbumin), SAL + rBmTI-A (control treated with rBmTI-A) and OVA + rBmTI-A (sensitized with ovalbumin and treated with rBmTI-A). The animals received an intraperitoneal injection of saline or ovalbumin, according to the group. The groups received inhalation with saline or ovalbumin and were treated with rBmTI-A or saline by nasal instillation. After 29 days, we evaluated the respiratory mechanics; bronchoalveolar lavage fluid (BALF); cytokines; MMP-9, TIMP-1; eosinophils; collagen and elastic fibre expression in the airways; and the trypsin-like, MMP-1, and MMP-9 lung tissue proteolytic activity. Treatment with rBmTI-A reduced the trypsin-like proteolytic activity, the elastance and resistance maximum response, the polymorphonuclear cells, IL-5, IL-10, IL-13 and IL-17A in the BALF, the expression of IL-5, IL-13, IL-17, CD4+, MMP-9, TIM P-1, eosinophils, collagen and elastic fibres in the airways of the OVA + rBmTI-A group compared to the OVA group (p < 0.05). rBmTI-A attenuated bronchial hyperresponsiveness, inflammation and remodelling in this experimental model of chronic allergic pulmonary inflammation. This inhibitor may serve as a potential therapeutic tool for asthma treatment.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/33950
Referências
  1. Almeida-Reis R, 2017, BIOMED RES INT, DOI 10.1155/2017/8287125
  2. Arantes-Costa FM, 2002, EUR RESPIR J, V19, P1008, DOI 10.1183/09031936.02.00232402
  3. Asaduzzaman M, 2015, CLIN EXP ALLERGY, V45, P1844, DOI 10.1111/cea.12628
  4. Atkinson JJ, 2003, AM J RESP CELL MOL, V28, P12, DOI 10.1165/rcmb.2002-0166TR
  5. Bellini A, 2012, MUCOSAL IMMUNOL, V5, P140, DOI 10.1038/mi.2011.60
  6. BOCHNER BS, 1994, ANNU REV IMMUNOL, V12, P295
  7. Bousquet J, 2000, AM J RESP CRIT CARE, V161, P1720, DOI 10.1164/ajrccm.161.5.9903102
  8. Brandenberger C, 2014, CLIN EXP ALLERGY, V44, P1282, DOI 10.1111/cea.12388
  9. Camelier Aquiles A, 2008, J. bras. pneumol., V34, P514, DOI 10.1590/S1806-37132008000700012
  10. Chambers LS, 2001, AM J PHYSIOL-LUNG C, V281, pL1369
  11. Dos Santos ASA, 2014, AM J RESP CRIT CARE, V189
  12. Fernandez-Rodriguez S, 2008, INT IMMUNOPHARMACOL, V8, P756, DOI 10.1016/j.intimp.2008.01.025
  13. Flood-Page P, 2003, J CLIN INVEST, V112, P1029, DOI 10.1172/JCI200317974
  14. GINA, GLOB STRAT ASTHM MAN
  15. Gueders MM, 2009, INFLAMM RES, V58, P845, DOI 10.1007/s00011-009-0054-2
  16. Gueders MM, 2006, EUR J PHARMACOL, V533, P133, DOI 10.1016/j.ejphar.2005.12.082
  17. Hamid Q., 2009, ANN REV PALO ALTO, P489
  18. Ishizaki M, 2008, J PHARMACOL SCI, V108, P355, DOI 10.1254/jphs.08162FP
  19. Kita H, 1996, AM J RESP CRIT CARE, V153, P1437, DOI 10.1164/ajrccm.153.4.8616578
  20. Lambrecht BN, 2015, NAT IMMUNOL, V16, P45, DOI 10.1038/ni.3049
  21. Leckie MJ, 2000, LANCET, V356, P2144, DOI 10.1016/S0140-6736(00)03496-6
  22. Leick-maldonado EA, 2004, CLIN EXP ALLERGY, V34, P145, DOI 10.1111/j.1365-2222.2004.01854.x
  23. Liang GY, 2016, CURR TOP MED CHEM, V16, P1506, DOI 10.2174/1568026615666150915121447
  24. Lin CC, 2014, MEDIAT INFLAMM, DOI 10.1155/2014/879326
  25. Lourenco J. D., 2017, HISTOL HISTOPATHOL, V11927
  26. Lourenco JD, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0098216
  27. MALEFYT RD, 1991, J EXP MED, V174, P915
  28. Martins-Olivera BT, 2016, MEDIAT INFLAMM, DOI 10.1155/2016/5346574
  29. Mattos W, 2002, CHEST, V122, P1543, DOI 10.1378/chest.122.5.1543
  30. Menzies-Gow A, 2003, J ALLERGY CLIN IMMUN, V111, P714, DOI 10.1067/mai.2003.1382
  31. Monteseirin J, 1992, Allergol Immunopathol (Madr), V20, P211
  32. Nishioka T, 2008, PROTEOM CLIN APPL, V2, P46, DOI 10.1002/prca.200780065
  33. Noel A, 2004, J CLIN PATHOL, V57, P577, DOI 10.1136/jcp.2003.014472
  34. Pabinger I, 1996, ARTERIOSCL THROM VAS, V16, P742, DOI 10.1161/01.ATV.16.6.742
  35. Papaioannou AI, 2015, EUR RESPIR REV, V24, P361, DOI 10.1183/16000617.00001615
  36. Prado CM, 2006, AM J RESP CELL MOL, V35, P457, DOI 10.1165/rcmb.2005-0391OC
  37. RighettI RF, 2014, RESP PHYSIOL NEUROBI, V192, P134, DOI 10.1016/j.resp.2013.12.012
  38. Righetti L, 2017, FOOD ADDIT CONTAM A, V34, P248, DOI 10.1080/19440049.2016.1265671
  39. Royce SG, 2012, MOL CELL ENDOCRINOL, V351, P167, DOI 10.1016/j.mce.2012.01.007
  40. Sasaki SD, 2004, BIOCHIMIE, V86, P643, DOI 10.1016/j.biochi.2004.09.010
  41. Sasaki SD, 2008, VET PARASITOL, V155, P133, DOI 10.1016/j.vetpar.2008.03.031
  42. Saw S, 2015, INFLAMMATION, V38, P672, DOI 10.1007/s10753-014-9976-0
  43. Schuijs MJ, 2013, CURR OPIN PHARMACOL, V13, P351, DOI 10.1016/j.coph.2013.03.013
  44. Shigetomi H, 2010, INFLAMM RES, V59, P679, DOI 10.1007/s00011-010-0205-5
  45. Tanaka AS, 1999, IMMUNOPHARMACOLOGY, V45, P171, DOI 10.1016/S0162-3109(99)00074-0
  46. Theodoro OA, 2017, INT J MOL SCI, V18, DOI 10.3390/ijms18020403
  47. Tiberio IFLC, 1997, AM J RESP CRIT CARE, V155, P1739, DOI 10.1164/ajrccm.155.5.9154886
  48. Toledo AC, 2013, BRIT J PHARMACOL, V168, P1736, DOI 10.1111/bph.12062
  49. Vieira RP, 2008, AM J PHYSIOL-LUNG C, V295, pL670, DOI 10.1152/ajplung.00465.2007
  50. Vignola AM, 1998, AM J RESP CRIT CARE, V157, P505, DOI 10.1164/ajrccm.157.2.9703070
  51. Vliagoftis A, 2000, J ALLERGY CLIN IMMUN, V106, P537, DOI 10.1067/mai.2000.109058
  52. Walters E H, 1996, Respirology, V1, P233, DOI 10.1111/j.1440-1843.1996.tb00039.x
  53. Weibel E.R., 1963, MORPHOMETRY HUMAN LU
  54. Weibel Ewald R, 2010, Nihon Kokyuki Gakkai Zasshi, V48, P637
  55. Zijlstra GJ, 2012, EUR RESPIR J, V39, P439, DOI 10.1183/09031936.00017911

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/20
Artigos e Materiais de Revistas Científicas - ODS/03