The molecular effects of electrical stimulation on the muscle components of the urethra of female rats after trauma by vaginal distention
Carregando...
Citações na Scopus
1
Tipo de produção
article
Data de publicação
2020
Editora
WILEY
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
SALERNO, Gisela R. F.
BORTOLINI, Maria A. T.
GOMES, Regina C. T.
FEITOSA, Suellen M.
SIMOES, Manuel J.
CASTANHO, Fernanda L.
CASTRO, Rodrigo A.
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
NEUROUROLOGY AND URODYNAMICS, v.39, n.2, p.576-585, 2020
Resumo
Aims To evaluate the expression of genes and proteins related to the urethral muscles of female rats after trauma by vaginal distention (VD) and after electrical stimulation therapy (EST). Methods We compared the urethras of four groups of 20 animals each: control without trauma (C), 7 (recent-trauma) and 30 days (late-trauma) post-VD, and VD-treated with EST. We evaluated the expression of myogenic regulatory factors MYOD1 and myogenin (MYOG); skeletal muscle myosin heavy chain 1, 2, and 3 (MYH1, MYH2, and MYH3); smooth muscle MYH11; and myosin light chain 9 (MYL9). We used real-time quantitative polymerase chain reaction, Western blot analysis, and immunohistochemistry. Results MYOD1 and MYOG genes were overexpressed in the recent-trauma group compared with the other groups (P < .05). MYH1 and MYH3 genes were upregulated in the recent-trauma group compared with the control and EST groups (P < .05). The MYH2 gene was overexpressed in the late-trauma group (P < .05), while the MYH2 protein was significantly increased in the EST group compared with control, recent-trauma and late-trauma groups by 5-, 3-, and 2.7-fold change, respectively (P < .05). MYL9 and MYH11 messenger RNA were overexpressed in both trauma groups compared with control and EST groups (P < .05). MYH11 protein was not different among the study groups (P > .05). Conclusions EST enhances the recovery of the damaged urethral tissue of rats mainly by acting on the striated-muscle components. The MYH2 pathway underlies the positive effects of EST in the external urethral sphincter.
Palavras-chave
electrical stimulation therapy, female rats, muscle, urethra, vaginal distension
Referências
- Ayeleke RO, 2015, COCHRANE DB SYST REV, DOI 10.1002/14651858.CD010551.pub3
- Beamish JA, 2010, TISSUE ENG PART B-RE, V16, P467, DOI [10.1089/ten.teb.2009.0630, 10.1089/ten.TEB.2009.0630]
- Bo K, 2017, INT UROGYNECOL J, V28, P191, DOI 10.1007/s00192-016-3123-4
- Bortolini MAT, 2019, INT UROGYNECOL J, V30, P465, DOI 10.1007/s00192-018-3634-2
- Chen Y, 2018, GASTROENT RES PRACT, DOI 10.1155/2018/6309157
- DELANCEY JOL, 1990, CLIN OBSTET GYNECOL, V33, P298, DOI 10.1097/00003081-199006000-00014
- Franco GR, 2011, REV ASSOC MED BRAS, V57, P20, DOI 10.1590/S0104-42302011000100010
- Gurnett CA, 2009, CLIN ORTHOP RELAT R, V467, P1195, DOI 10.1007/s11999-008-0694-5
- Haylen BT, 2010, NEUROUROL URODYNAM, V29, P4, DOI 10.1002/nau.20798
- Hofer MD, 2014, UROLOGY, V84, P246, DOI 10.1016/j.urology.2014.04.012
- Hoppeler H, 2003, MED SCI SPORT EXER, V35, P95, DOI 10.1097/00005768-200301000-00016
- Huard J, 2002, J BONE JOINT SURG AM, V84A, P822, DOI 10.2106/00004623-200205000-00022
- Jung J, 2012, INT NEUROUROL J, V16, P102, DOI 10.5213/inj.2012.16.3.102
- Lee In Sook, 2006, Taehan Kanho Hakhoe Chi, V36, P1374
- Lim SH, 2013, ANAT REC, V296, P1640, DOI 10.1002/ar.22740
- Mastroyiannopoulos NP, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0029896
- Miyata T, 2009, J PHYS THER SCI, V21, P379, DOI 10.1589/jpts.21.379
- PHILLIPS JI, 1980, EXP GERONTOL, V15, P551, DOI 10.1016/0531-5565(80)90008-X
- Rocha MA, 2007, INT UROGYNECOL J, V18, P1453, DOI 10.1007/s00192-007-0355-3
- Russo TL, 2010, J MUSCLE RES CELL M, V31, P45, DOI 10.1007/s10974-010-9203-z
- Salmons S, 2008, ARTIF ORGANS, V32, P597, DOI 10.1111/j.1525-1594.2008.00608.x
- Schiaffino S, 2011, PHYSIOL REV, V91, P1447, DOI 10.1152/physrev.00031.2010
- Stewart F, 2017, COCHRANE DB SYST REV, DOI 10.1002/14651858.CD012390.pub2
- Takano CC, 2009, INT UROGYNECOL J, V21, P705
- Valenzuela PL, 2017, ARCH BIOL SCI, V69, P391, DOI 10.2298/ABS160925110V