25-vitamin D reduces inflammation in uremic environment
Carregando...
Citações na Scopus
14
Tipo de produção
article
Data de publicação
2020
Título da Revista
ISSN da Revista
Título do Volume
Editora
NATURE PUBLISHING GROUP
Autores
BRITO, Rodrigo Barbosa de Oliveira
REBELLO, Jacqueline Ferritto
GRABULOSA, Caren Cristina
PINTO, Walter
JR, Armando Morales
DALBONI, Maria Aparecida
Citação
SCIENTIFIC REPORTS, v.10, n.1, article ID 128, 8p, 2020
Resumo
Chronic kidney disease (CKD) is characterized by loss of renal function and a consequent increase of serum uremic toxins, which contribute to inflammation status. Deficiency of 25-vitamin D, often found in patients with CKD, has been included as an inflammatory factor since it might modulate the immune system. The aim of this study was to investigate the role of 25-vitamin D on inflammatory pathways in healthy and uremic environment. Toll-like receptor 4 (TLR4), oxidative stress (ROS), vitamin D receptor (VDR), 1-alpha hydroxylase (CYP27), 24 hydroxylase, cathelicidin, and MCP-1 were evaluated in monocytes exposed to a uremic serum pool compared with healthy pool. The human monocytes lineage (U937) was incubated with or without 25-vitamin D (50 ng/ml for 24 hours). TRL4, VDR, CYP27, CYP24, and ROS were evaluated by flow cytometry. We used ELISA to measure IL-6, TNF-alpha, IL-10, cathelicidin, and MCP-1 in the cell culture supernatant. We observed a higher expression of TRL-4, IL-6, TNF-alpha, IL-10, cathelicidin and MCP-1 in monocytes incubated with uremic serum when compared with serum from healthy individuals. Supplementation of 25-vitamin D was able to reduce the expression of TRL4, cathelicidin, and MCP-1 in the uremic environment. There was no difference in the expression of VDR, CYP27 and CYP24 intracellular enzymes. This in vitro study showed that the uremic pool activates inflammatory response in monocytes, which was reversed by 25-vitamin D supplementation; this finding suggests that 25-vitamin D has an anti-inflammatory role in the uremic environment.
Palavras-chave
Referências
- Akira S, 2004, NAT REV IMMUNOL, V4, P499, DOI 10.1038/nri1391
- Azevedo MLV, 2016, TOXICOL LETT, V263, P1, DOI 10.1016/j.toxlet.2016.10.006
- BHALLA AK, 1983, J CLIN ENDOCR METAB, V57, P1308, DOI 10.1210/jcem-57-6-1308
- Bhavsar P, 2008, THORAX, V63, P784, DOI 10.1136/thx.2007.090027
- Bolati D, 2013, BMC NEPHROL, V14, DOI 10.1186/1471-2369-14-56
- Borges NA, 2016, J RENAL NUTR, V26, P396, DOI 10.1053/j.jrn.2016.07.005
- Carmona A, 2017, FRONT PHYSIOL, V8, DOI 10.3389/fphys.2017.00666
- Carvalho JTG, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0179540
- Charytan DM, 2015, AM J KIDNEY DIS, V66, P196, DOI 10.1053/j.ajkd.2014.12.016
- Claro LM, 2018, TOXINS, V10, DOI 10.3390/toxins10100384
- da Silva FP, 2017, IMMUNOL LETT, V182, P57, DOI 10.1016/j.imlet.2017.01.004
- Fukami A, 2011, CLIN CARDIOL, V34, P189, DOI 10.1002/clc.20834
- Go AS, 2004, NEW ENGL J MED, V351, P1296, DOI 10.1056/NEJMoa041031
- Gois PHF, 2018, INT J ENV RES PUB HE, V15, DOI 10.3390/ijerph15081773
- Gombart AF, 2007, J STEROID BIOCHEM, V103, P552, DOI 10.1016/j.jsbmb.2006.12.095
- Grabulosa CC, 2018, EXP CELL RES, V365, P157, DOI 10.1016/j.yexcr.2018.02.022
- HASUI M, 1989, J IMMUNOL METHODS, V117, P53, DOI 10.1016/0022-1759(89)90118-X
- Holick MF, 2007, NEW ENGL J MED, V357, P266, DOI 10.1056/NEJMra070553
- Holick MF, 2017, REV ENDOCR METAB DIS, V18, P153, DOI 10.1007/s11154-017-9424-1
- Jain SK, 2013, BIOCHEM BIOPH RES CO, V437, P7, DOI 10.1016/j.bbrc.2013.06.004
- Kaminski TW, 2017, BMC NEPHROL, V18, DOI 10.1186/s12882-017-0457-1
- Kim HY, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-03130-z
- Kracht M, 2002, CYTOKINE, V20, P91, DOI 10.1006/cyto.2002.0895
- Lau WL, 2018, CLIN SCI, V132, P509, DOI 10.1042/CS20171107
- Lekawanvijit S, 2014, J PHYSIOL-LONDON, V592, P3969, DOI 10.1113/jphysiol.2014.273078
- Liu PT, 2006, SCIENCE, V311, P1770, DOI 10.1126/science.1123933
- Meireles MS, 2016, CLIN NUTR, V35, P1251, DOI 10.1016/j.clnu.2016.04.014
- Mousa A, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-15264-1
- Onal EM, 2019, HYPERTENS RES, V42, P123, DOI 10.1038/s41440-018-0144-z
- Outtz HH, 2010, J IMMUNOL, V185, P4363, DOI 10.4049/jimmunol.1000720
- PARRILLO JE, 1993, NEW ENGL J MED, V328, P1471, DOI 10.1056/nejm199305203282008
- Ramanathan B, 2002, MICROBES INFECT, V4, P361, DOI 10.1016/S1286-4579(02)01549-6
- Rossi M, 2014, ARCH MED RES, V45, P309, DOI 10.1016/j.arcmed.2014.04.002
- Shimizu H, 2013, LIFE SCI, V92, P143, DOI 10.1016/j.lfs.2012.11.012
- Sorensen OE, 2001, BLOOD, V97, P3951, DOI 10.1182/blood.V97.12.3951
- Stinghen AEM, 2011, INT J HYPERTENS, DOI 10.4061/2011/232683
- Stockler-Pinto MB, 2018, INT UROL NEPHROL, V50, P347, DOI 10.1007/s11255-017-1748-y
- STUMPF WE, 1979, SCIENCE, V206, P1188, DOI 10.1126/science.505004
- Trojanowicz B, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0102137
- Vanholder Raymond C, 2003, Hemodial Int, V7, P156, DOI 10.1046/j.1492-7535.2003.00034.x
- Yang LF, 2017, J TOXICOL SCI, V42, P763, DOI 10.2131/jts.42.763
- Zhang GL, 2001, J CLIN INVEST, V107, P13, DOI 10.1172/JCI11837
- Zhang Y, 2012, J IMMUNOL, V188, P2127, DOI 10.4049/jimmunol.1102412