Phage Display Identification of CD100 in Human Atherosclerotic Plaque Macrophages and Foam Cells

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP LUQUE, Maria Carolina Aquino
MARTINS, Waleska Kerllen
PORTO, Georgia
BOUMSELL, Laurence
STOLF, Beatriz 2013
dc.identifier.citation PLOS ONE, v.8, n.9, article ID e75772, 9p, 2013
dc.identifier.issn 1932-6203
dc.description.abstract Atherosclerosis is a complex disease in which vessels develop plaques comprising dysfunctional endothelium, monocyte derived lipid laden foam cells and activated lymphocytes. Considering that humans and animal models of the disease develop quite distinct plaques, we used human plaques to search for proteins that could be used as markers of human atheromas. Phage display peptide libraries were probed to fresh human carotid plaques, and a bound phage homologous to plexin B1, a high affinity receptor for CD100, was identified. CD100 is a member of the semaphorin family expressed by most hematopoietic cells and particularly by activated T cells. CD100 expression was analyzed in human plaques and normal samples. CD100 mRNA and protein were analyzed in cultured monocytes, macrophages and foam cells. The effects of CD100 in oxLDL-induced foam cell formation and in CD36 mRNA abundance were evaluated. Human atherosclerotic plaques showed strong labeling of CD100/SEMA4D. CD100 expression was further demonstrated in peripheral blood monocytes and in in vitro differentiated macrophages and foam cells, with diminished CD100 transcript along the differentiation of these cells. Incubation of macrophages with CD100 led to a reduction in oxLDL-induced foam cell formation probably through a decrease of CD36 expression, suggesting for the first time an atheroprotective role for CD100 in the human disease. Given its differential expression in the numerous foam cells and macrophages of the plaques and its capacity to decrease oxLDL engulfment by macrophages we propose that CD100 may have a role in atherosclerotic plaque development, and may possibly be employed in targeted treatments of these atheromas.
dc.description.sponsorship · FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
dc.language.iso eng
dc.relation.ispartof Plos One
dc.rights openAccess
dc.subject.other surface expression; scavenger receptors; soluble cd100; activation; lesions; growth; gamma
dc.title Phage Display Identification of CD100 in Human Atherosclerotic Plaque Macrophages and Foam Cells
dc.type article
dc.rights.holder Copyright PUBLIC LIBRARY SCIENCE LIM/19 LIM/28 LIM/02
dc.identifier.doi 10.1371/journal.pone.0075772
dc.identifier.pmid 24098722
dc.type.category original article
dc.type.version publishedVersion GUTIERREZ, Paulo Sampaio:HC:INCOR DEBBAS, Victor:FM:MCP PUECH-LEAO, Pedro:FM:MCG COELHO, Veronica:HC:INCOR KALIL, Jorge:FM:MCM · LUQUE, Maria Carolina Aquino:HC FMUSP, Heart Inst Sao Paulo InCor, Sao Paulo, Brazil; Univ Sao Paulo, Sch Med, Dept Clin Med, HC FMUSP, Sao Paulo, Brazil
· MARTINS, Waleska Kerllen:Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, Brazil
· PORTO, Georgia:HC FMUSP, Heart Inst Sao Paulo InCor, Sao Paulo, Brazil
· BOUMSELL, Laurence:Univ Paris 05, Inst Cochin, INSERM, U567, Paris, France
· STOLF, Beatriz:HC FMUSP, Heart Inst Sao Paulo InCor, Sao Paulo, Brazil; Univ Sao Paulo, Sch Med, Dept Clin Med, HC FMUSP, Sao Paulo, Brazil; Univ Sao Paulo, Dept Parasitol, Inst Biomed Sci, Sao Paulo, Brazil 2-s2.0-84884738667 WOS:000325423500093 SAN FRANCISCO USA
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