A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author FERREIRA, Camila da Silva FMUSP-HC
ABREU, Rodrigo Martins FMUSP-HC
SILVA, Marlone Cunha da
FERREIRA, Aline Siqueira
NASSER, Paulo Dominguez FMUSP-HC
CARRILHO, Flair Jose FMUSP-HC
ONO, Suzane Kioko FMUSP-HC
dc.date.issued 2013
dc.identifier.citation PLOS ONE, v.8, n.10, article ID e78142, 4p, 2013
dc.identifier.issn 1932-6203
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/4057
dc.description.abstract Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-lambda-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods.
dc.description.sponsorship · Alves de Queiroz Family Foundation
dc.language.iso eng
dc.publisher PUBLIC LIBRARY SCIENCE
dc.relation.ispartof Plos One
dc.rights openAccess
dc.subject.other genetic-variation; virus-infection; il28b; clearance; variants
dc.title A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
dc.type article
dc.rights.holder Copyright PUBLIC LIBRARY SCIENCE
dc.description.group LIM/07
dc.identifier.doi 10.1371/journal.pone.0078142
dc.identifier.pmid 24167602
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author FERREIRA, Camila da Silva:FM:
hcfmusp.author ABREU, Rodrigo Martins:FM:
hcfmusp.author NASSER, Paulo Dominguez:FM:
hcfmusp.author CARRILHO, Flair Jose:FM:MGT
hcfmusp.author ONO, Suzane Kioko:FM:MGT
hcfmusp.author.external · SILVA, Marlone Cunha da:Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
· FERREIRA, Aline Siqueira:Univ Fed Juiz de Fora, Dept Parasitol Microbiol & Immunol, Inst Biol Sci, Juiz De Fora, MG, Brazil
hcfmusp.origem.id 2-s2.0-84885987736
hcfmusp.origem.id WOS:000326034500062
hcfmusp.publisher.city SAN FRANCISCO
hcfmusp.publisher.country USA
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dc.description.index MEDLINE
hcfmusp.citation.scopus 5
hcfmusp.citation.wos 4
hcfmusp.affiliation.country Brasil


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