APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author SATOW, Marcela M. FMUSP-HC
YAMASHIRO-KANASHIRO, Edite H. FMUSP-HC
ROCHA, Mussya C. FMUSP-HC
OYAFUSO, Luiza K.
SOLER, Rita C.
COTRIM, Paulo C. FMUSP-HC
LINDOSO, Jose Angelo L. FMUSP-HC
dc.date.issued 2013
dc.identifier.citation REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.55, n.6, p.393-399, 2013
dc.identifier.issn 0036-4665
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/4076
dc.description.abstract This study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emolio Ribas (IIER), a reference center for infectious diseases in Sao Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test, 61.8% (47/76) for direct investigation, and 19.3% (22/114) for in vitro culture. The molecular method was able to confirm the disease in samples considered negative or inconclusive by traditional laboratory methods, contributing to the final clinical diagnosis and therapy of ATL in this hospital. Thus, we strongly recommend the inclusion of kDNA-PCR amplification as an alternative diagnostic method for ATL, suggesting a new algorithm routine to be followed to help the diagnosis and treatment of ATL in IIER.
dc.description.abstract Este estudo avaliou a aplicabilidade do kDNA-PCR como método de rotina para diagnóstico de leishmaniose tegumentar americana (ATL) no Instituto de Infectologia Emílio Ribas (IIER), São Paulo, SP, Brasil. O método kDNA-PCR detectou DNA de Leishmania em 87,5% (112/128) dos pacientes com suspeita de ter leishmaniose e, os métodos tradicionais apresentaram as seguintes porcentagens de positividade: 62,8% (49/78) para o teste de Montenegro, 61,8% (47/76) para a pesquisa direta e 19,3% (22/114) para cultura in vitro. O método molecular confirmou a doença em amostras negativas ou inconclusivas pelos métodos laboratoriais tradicionais e, mostrou-se capaz de auxiliar na identificação de infecções causadas pela espécie Leishmania (V.) braziliensis. Além disso, a revisão dos prontuários médicos confirmou a importância do método PCR-RFLP no diagnóstico final de ATL, prognóstico e escolha do tratamento. Assim, recomendamos a inclusão do PCR como método diagnóstico de ATL na rotina hospitalar, e sugerimos um fluxograma para solicitação de exames laboratoriais
dc.description.sponsorship · Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP [2010/16963-4]
· Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
· CNPq
· Laboratorio de Investigacao Medica [FMUSP-HC/LIM-38 e LIM-48]
dc.language.iso eng
dc.publisher INST MEDICINA TROPICAL SAO PAULO
dc.relation.ispartof Revista do Instituto de Medicina Tropical de Sao Paulo
dc.rights openAccess
dc.subject American tegumentary leishmaniasis; Diagnostic; kDNA-PCR; Leishmania (Viannia) braziliensis
dc.subject.other polymerase-chain-reaction; cutaneous leishmaniasis; mucocutaneous leishmaniasis; viannia braziliensis; identification; performance; argentina
dc.title APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL
dc.title.alternative Aplicação do kDNA-PCR para diagnóstico de rotina de leishmaniose tegumentar americana em um hospital de referência
dc.type article
dc.rights.holder Copyright INST MEDICINA TROPICAL SAO PAULO
dc.description.group LIM/48
dc.description.group LIM/38
dc.identifier.doi 10.1590/S0036-46652013000600004
dc.identifier.pmid 24213191
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author SATOW, Marcela M.:IMT:
hcfmusp.author YAMASHIRO-KANASHIRO, Edite H.:HC:LIM/48
hcfmusp.author ROCHA, Mussya C.:HC:LIM/48
hcfmusp.author COTRIM, Paulo C.:FM:MIP
hcfmusp.author LINDOSO, Jose Angelo L.:HC:LIM/38
hcfmusp.author.external · OYAFUSO, Luiza K.:Inst Infectol Emilio Ribas, Sao Paulo, Brazil
· SOLER, Rita C.:Inst Infectol Emilio Ribas, Sao Paulo, Brazil
hcfmusp.origem.id WOS:000326974700004
hcfmusp.origem.id 2-s2.0-84887335021
hcfmusp.origem.id SCIELO:S0036-46652013000600393
hcfmusp.publisher.city SAO PAULO
hcfmusp.publisher.country BRAZIL
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dc.description.index MEDLINE
hcfmusp.citation.scopus 10
hcfmusp.citation.wos 5
hcfmusp.affiliation.country Brasil


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