Low Sensitivity of NS1 Protein Tests Evidenced during a Dengue Type 2 Virus Outbreak in Santos, Brazil, in 2010

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art_FELIX_Low_Sensitivity_of_NS1_Protein_Tests_Evidenced_during_2012.PDF (301.55 KB)
Citações na Scopus
34
Tipo de produção
article
Data de publicação
2012
Editora
AMER SOC MICROBIOLOGY
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
MARTELLI, Celina Maria Turchi
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
CLINICAL AND VACCINE IMMUNOLOGY, v.19, n.12, p.1972-1976, 2012
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary infection by dengue 2 virus. Sequencing of NS1 positive and negative isolates did not reveal any mutation that could justify the diagnostic failure. Use of existing NS1 tests in the Brazilian population may present a low negative predictive value, and they should be used with caution, preferentially after performing a validation with samples freshly obtained during the ongoing epidemic.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/421
Referências
  1. Alcon S, 2002, J CLIN MICROBIOL, V40, P376, DOI 10.1128/JCM.40.2.376-381.2002
  2. Barreto ML, 2011, LANCET, V377, P1877, DOI 10.1016/S0140-6736(11)60202-X
  3. Castro-Jorge LA, 2010, J MED VIROL, V82, P1400, DOI 10.1002/jmv.21814
  4. CHAMBERS TJ, 1990, ANNU REV MICROBIOL, V44, P649, DOI 10.1146/annurev.micro.44.1.649
  5. Coller BAG, 2011, CURR OPIN IMMUNOL, V23, P391, DOI 10.1016/j.coi.2011.03.005
  6. de Souza VAUF, 2004, J CLIN MICROBIOL, V42, P1782, DOI 10.1128/JCM.42.4.1782-1784.2004
  7. Duyen HTL, 2011, J INFECT DIS, V203, P1292, DOI 10.1093/infdis/jir014
  8. Guilarde AO, 2008, J INFECT DIS, V197, P817, DOI 10.1086/528805
  9. Guzman MG, 2010, PLOS NEGLECT TROP D, V4, DOI 10.1371/journal.pntd.0000811
  10. Hang VT, 2009, PLOS NEGLECT TROP D, V3, DOI 10.1371/journal.pntd.0000360
  11. Harris E, 1998, J CLIN MICROBIOL, V36, P2634
  12. Kao Chuan-Liang, 2005, Journal of Microbiology Immunology and Infection, V38, P5
  13. Koraka P, 2003, J CLIN MICROBIOL, V41, P4154, DOI 10.1128/JCM.41.9.4154-4159.2003
  14. Levi JE, 2007, J CLIN MICROBIOL, V45, P1893, DOI 10.1128/JCM.00065-07
  15. Libraty DH, 2002, J INFECT DIS, V186, P1165, DOI 10.1086/343813
  16. Lima MDQ, 2010, PLOS NEGLECT TROP D, V4, DOI 10.1371/journal.pntd.0000738
  17. Romano CM, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0011811
  18. Secretaria da Saude do Estado de Sao Paulo, OFF DAT DENG EP STAT
  19. Simmons CP, 2012, NEW ENGL J MED, V366, P1423, DOI 10.1056/NEJMra1110265
  20. Stramer SL, 2009, TRANSFUSION, V49, p1S, DOI 10.1111/j.1537-2995.2009.02279.x
  21. Stramer SL, 2012, TRANSFUSION, V52, P1657, DOI 10.1111/j.1537-2995.2012.03566.x
  22. Tambyah PA, 2008, NEW ENGL J MED, V359, P1526, DOI 10.1056/NEJMc0708673
  23. Teixeira MG, 2012, REV INST MED TROP SP, V54, pS1, DOI 10.1590/S0036-46652012000700002
  24. Teles FRR, 2005, REV MED VIROL, V15, P287, DOI 10.1002/rmv.461
  25. WHO, WORLD STAT DENG CAS
  26. Young PR, 2000, J CLIN MICROBIOL, V38, P1053

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/47
Artigos e Materiais de Revistas Científicas - LIM/52
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