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https://observatorio.fm.usp.br/handle/OPI/44578
Title: | Latency-associated DNA methylation patterns among HIV-1 infected individuals with distinct disease progression courses or antiretroviral virologic response |
Authors: | MANTOVANI, Nathalia; DEFELICIBUS, Alexandre; SILVA, Israel Tojal da; CICERO, Maira Ferreira; SANTANA, Luiz Claudio; ARNOLD, Rafael; CASTRO, Daniela Funayama de; DURO, Rodrigo Lopes Sanz; NISHIYAMA-JR, Milton Yutaka; JUNQUEIRA-DE-AZEVEDO, Inacio Loiola Meirelles; SILVA, Bosco Christiano Maciel da; DUARTE, Alberto Jose da Silva; CASSEB, Jorge; TENORE, Simone de Barros; HUNTER, James; DIAZ, Ricardo Sobhie; KOMNINAKIS, Shirley Cavalcante Vasconcelos |
Citation: | SCIENTIFIC REPORTS, v.11, n.1, article ID 22993, 15p, 2021 |
Abstract: | DNA methylation is one of the epigenetic modifications that configures gene transcription programs. This study describes the DNA methylation profile of HIV-infected individuals with distinct characteristics related to natural and artificial viremia control. Sheared DNA from circulating mononuclear cells was subjected to target enrichment bisulfite sequencing designed to cover CpG-rich genomic regions. Gene expression was assessed through RNA-seq. Hypermethylation in virologic responders was highly distributed closer to Transcription Start Sites (p-value = 0.03). Hyper and hypomethylation levels within TSS adjacencies varied according to disease progression status (Kruskal-Wallis, p < 0.001), and specific differentially methylated regions associated genes were identified for each group. The lower the promoter methylation, the higher the gene expression in subjects undergoing virologic failure (R = - 0.82, p = 0.00068). Among the inversely correlated genes, those supporting glycolysis and its related pathways were hypomethylated and up-regulated in virologic failures. Disease progression heterogeneity was associated with distinct DNA methylation patterns in terms of rates and distribution. Methylation was associated with the expression of genes sustaining intracellular glucose metabolism in subjects undergoing antiretroviral virologic failure. Our findings highlight that DNA methylation is associated with latency, disease progression, and fundamental cellular processes. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/56 Artigos e Materiais de Revistas Científicas - ODS/03 |
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File | Description | Size | Format | |
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art_MANTOVANI_Latencyassociated_DNA_methylation_patterns_among_HIV1_infected_individuals_2021.PDF | publishedVersion (English) | 5.45 MB | Adobe PDF | View/Open |
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