Stem Cells as a Good Tool to Investigate Dysregulated Biological Systems in Autism Spectrum Disorders

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art_VADASZ_Stem_Cells_as_a_Good_Tool_to_Investigate_2013.PDF (722.92 KB)
Citações na Scopus
10
Tipo de produção
article
Data de publicação
2013
Editora
WILEY-BLACKWELL
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
GRIESI-OLIVEIRA, Karina
SUNAGA, Daniele Yumi
ALVIZI, Lucas
PASSOS-BUENO, Maria Rita
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
AUTISM RESEARCH, v.6, n.5, p.354-361, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Identification of the causes of autism spectrum disorders (ASDs) is hampered by their genetic heterogeneity; however, the different genetic alterations leading to ASD seem to be implicated in the disturbance of common molecular pathways or biological processes. In this scenario, the search for differentially expressed genes (DEGs) between ASD patients and controls is a good alternative to identify the molecular etiology of such disorders. Here, we employed genome-wide expression analysis to compare the transcriptome of stem cells of human exfoliated deciduous teeth (SHEDs) of idiopathic autistic patients (n=7) and control samples (n=6). Nearly half of the 683 identified DEGs are expressed in the brain (P=0.003), and a significant number of them are involved in mechanisms previously associated with ASD such as protein synthesis, cytoskeleton regulation, cellular adhesion and alternative splicing, which validate the use of SHEDs to disentangle the causes of autism. Autistic patients also presented overexpression of genes regulated by androgen receptor (AR), and AR itself, which in turn interacts with CHD8 (chromodomain helicase DNA binding protein 8), a gene recently shown to be associated with the cause of autism and found to be upregulated in some patients tested here. These data provide a rationale for the mechanisms through which CHD8 leads to these diseases. In summary, our results suggest that ASD share deregulated pathways and revealed that SHEDs represent an alternative cell source to be used in the understanding of the biological mechanisms involved in the etiology of ASD. Autism Res 2013, ..: ..-... (c) 2013 International Society for Autism Research, Wiley Periodicals, Inc.
Palavras-chave
expression studies, androgen signaling, CHD8, stem cells of human exfoliated deciduous teeth
URI
https://observatorio.fm.usp.br/handle/OPI/4570
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Coleções
Artigos e Materiais de Revistas Científicas - HC/IPq