Fat Grafts Supplemented with Adipose-Derived Stromal Cells in the Rehabilitation of Patients with Craniofacial Microsomia
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111
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article
Data de publicação
2013
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LIPPINCOTT WILLIAMS & WILKINS
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PLASTIC AND RECONSTRUCTIVE SURGERY, v.132, n.1, p.141-152, 2013
Resumo
Background: Although first reports of the clinical use of adipose-derived stromal cells suggest that this approach may be feasible and effective for soft-tissue augmentation, there is a lack of randomized, controlled clinical trials in the literature. Thus, this study aimed to investigate whether a faster protocol for isolation of adipose-derived stromal cells and their use in combination with fat tissue improve the long-term retention of the grafts in patients with craniofacial microsomia. Methods: Patients with craniofacial microsomia (n = 14) were grafted either with supplementation of adipose-derived stromal cells (experimental group) or without supplementation of adipose-derived stromal cells (control group). The number of viable cells isolated before and after the supplementation of the grafts was calculated, and these cells were examined for mesenchymal cell surface markers using flow cytometry. Computed tomography was performed to assess both hemifaces preoperatively and at 6 months postoperatively. Results: The average number of viable cells isolated before and after the supplementation of the grafts was 5.6 x 10(5) and 9.9 x 10(5) cells/ml of fat tissue (p = 0.015). Flow cytometric analysis revealed that the adipose-derived stromal cells were positive for mesenchymal cell markers (>95 percent for CD73 and CD105). Surviving fat volume at 6 months was 88 percent for the experimental group and 54 percent for the control group (p = 0.003). Conclusion: These results suggest that this strategy for isolation and supplementation of adipose-derived stromal cells is effective, safe, and superior to conventional lipoinjection for facial recontouring in patients with craniofacial microsomia.
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Referências
- Aguena M, 2012, STEM CELLS INT, V2012
- Coleman SR, 2006, PLAST RECONSTR SURG, V118, p108S, DOI 10.1097/01.prs.0000234610.81672.e7
- Conde-Green A, 2010, AESTHET SURG J, V30, P249, DOI 10.1177/1090820X10369512
- Eto H, 2009, PLAST RECONSTR SURG, V124, P1087, DOI 10.1097/PRS.0b013e3181b5a3f1
- Faustini M, 2010, TISSUE ENG PART C-ME, V16, P1515, DOI 10.1089/ten.TEC.2010.0214
- Fontdevila Joan, 2008, Aesthet Surg J, V28, P380, DOI 10.1016/j.asj.2008.05.002
- Fraser JK, 2006, TRENDS BIOTECHNOL, V24, P150, DOI 10.1016/j.tibtech.2006.01.010
- Goldenberg DC, 2007, J CRANIOFAC SURG, V18, P302, DOI 10.1097/scs.0b013e3180336012
- Gougoutas AJ, 2007, PLAST RECONSTR SURG, V120, p112E, DOI 10.1097/01.prs.0000287383.35963.5e
- Hamed S, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0013986
- HORGAN JE, 1995, CLEFT PALATE-CRAN J, V32, P405, DOI 10.1597/1545-1569(1995)032<0405:OPAOCA>2.3.CO;2
- Inigo F, 2000, MICROSURG, V20, P167, DOI 10.1002/1098-2752(2000)20:4<167::AID-MICR4>3.0.CO;2-D
- Josse C, 2010, STEM CELLS DEV, V19, P1167, DOI 10.1089/scd.2009.0264
- Karaaltin MV, 2012, J CRANIOFAC SURG, V23, pE103, DOI 10.1097/SCS.0b013e3182418ce8
- Kato H, 2010, TISSUE ENG PT A, V16, P2029, DOI 10.1089/ten.TEA.2009.0551
- Kaufman MR, 2007, PLAST RECONSTR SURG, V119, P2287, DOI 10.1097/01.prs.0000260712.44089.e7
- Kaufman MR, 2007, PLAST RECONSTR SURG, V119, P323, DOI 10.1097/01.prs.0000244903.51440.8c
- Khouri R, 2009, CLIN PLAST SURG, V36, P269, DOI 10.1016/j.cps.2008.11.009
- Kim G, 2012, J DIGIT IMAGING, V25, P486, DOI 10.1007/s10278-012-9458-6
- Koshima I, 2005, PLAST RECONSTR SURG, V116, P1091, DOI 10.1097/01.prs.0000178794.11828.49
- Kuroda Y, 2010, P NATL ACAD SCI USA, V107, P8639, DOI 10.1073/pnas.0911647107
- LI LT, 2011, IFATS MIAM 2011 9 AN
- LONGAKER MT, 1995, PLAST RECONSTR SURG, V96, P800, DOI 10.1097/00006534-199509001-00006
- Longaker MT, 1996, PLAST RECONSTR SURG, V98, P942, DOI 10.1097/00006534-199611000-00003
- Masuda T, 2004, TISSUE ENG, V10, P1672, DOI 10.1089/ten.2004.10.1672
- Matsumoto D, 2006, TISSUE ENG, V12, P3375, DOI 10.1089/ten.2006.12.3375
- Mojallal A, 2009, PLAST RECONSTR SURG, V124, P471, DOI 10.1097/PRS.0b013e3181af023a
- Moseley TA, 2006, PLAST RECONSTR SURG, V118, p121S, DOI 10.1097/01.prs.0000234609.74811.2e
- Padoin AV, 2008, PLAST RECONSTR SURG, V122, P614, DOI 10.1097/PRS.0b013e31817d5476
- Rohrich RJ, 2011, PLAST RECONSTR SURG, V127, p3S, DOI 10.1097/PRS.0b013e31820c2421
- Rubio D, 2005, CANCER RES, V65, P3035
- Saadeh Pierre B, 2008, Plast Reconstr Surg, V121, p368e, DOI 10.1097/PRS.0b013e3181707194
- Spalding KL, 2008, NATURE, V453, P783, DOI 10.1038/nature06902
- Suga H, 2008, PLAST RECONSTR SURG, V122, P103, DOI 10.1097/PRS.0b013e31817742ed
- Suga H, 2009, STEM CELLS, V27, P238, DOI 10.1634/stemcells.2008-0261
- Tabit CJ, 2012, AESTHET PLAST SURG, V36, P704, DOI 10.1007/s00266-011-9835-4
- Tanna N, 2011, PLAST RECONSTR SURG, V127, P802, DOI 10.1097/PRS.0b013e3181fed6e4
- Tiryaki T, 2011, AESTHET PLAST SURG, V35, P965, DOI 10.1007/s00266-011-9716-x
- Yoshimura K, 2010, BREAST J, V16, P169, DOI 10.1111/j.1524-4741.2009.00873.x
- Yoshimura K, 2008, DERMATOL SURG, V34, P1178, DOI 10.1111/j.1524-4725.2008.34256.x
- YOSHIMURA K, 2005, IFATS 2005 3 ANN S A
- Yoshimura K, 2009, REGEN MED, V4, P265, DOI 10.2217/17460751.4.2.265
- Yoshimura K, 2008, AESTHET PLAST SURG, V32, P48, DOI 10.1007/s00266-007-9019-4
- Zhang SJ, 2010, CHEM-BIOL INTERACT, V188, P119, DOI 10.1016/j.cbi.2010.06.008
- Zhu M, 2010, ANN PLAS SURG, V64, P222, DOI 10.1097/SAP.0b013e31819ae05c