Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)

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bookPart
Data de publicação
2015
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Scopus
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SPRINGER NEW YORK
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Alves, C. J.; Guimarães, A. B.; Maximino, J. R.; Bydlowski, S. P.; Chadi, G.. Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS). In: . IMMUNOCYTOCHEMISTRY AND RELATED TECHNIQUES: SPRINGER NEW YORK, 2015. p.195-208.
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Olfactory ensheathing cells ( OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis ( ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors ( p75NTR ), are plated onto laminin-coated coverslips for immunofluorescence characterization, or Petri dishes for expansion and further investigations. © Springer Science+Business Media New York 2015. All rights are reserved.
Palavras-chave
Amyotrophic lateral sclerosis, Characterization, Mouse, Neurodegeneration, Olfactory ensheathing glia, Olfactory mucosa, Purification, Repair, Therapy
URI
https://observatorio.fm.usp.br/handle/OPI/51051
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Coleções
Livros e Capítulos de Livros - FM/MCM
Livros e Capítulos de Livros - FM/MNE
Livros e Capítulos de Livros - LIM/31
Livros e Capítulos de Livros - LIM/45