Saliva as a source of anti-Toxoplasma gondii IgG for enzyme immunoassay in human samples

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author SAMPAIO, B. F. C.
MACRE, M. S.
MEIRELES, L. R. FMUSP-HC
ANDRADE JR., H. F. FMUSP-HC
dc.date.issued 2014
dc.identifier.citation CLINICAL MICROBIOLOGY AND INFECTION, v.20, n.1, p.O72-O74, 2014
dc.identifier.issn 1198-743X
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/5550
dc.description.abstract Toxoplasmosis, a highly prevalent disease, is mainly diagnosed by serology. Incidence studies could be feasible in children, but ethical concerns restrict blood sampling in this group. Saliva contains small amounts of crevicular fluid IgG. Dot-ELISA and a protein A IgG capture immunoassay were standardized for anti-Toxoplasma gondii IgG in paired saliva and serum samples from 20 adult volunteers. A frequency of toxoplasmosis of 19% (95% CI 12-28) was found in 100 saliva samples from university graduates using both assays. Toxoplasmosis immunoassays using saliva IgG are a promising tool for the investigation of the epidemiology of this disease in children and other vulnerable groups.
dc.description.sponsorship · [FMUSP-HC/LIMs]
dc.language.iso eng
dc.publisher WILEY-BLACKWELL
dc.relation.ispartof Clinical Microbiology and Infection
dc.rights restrictedAccess
dc.subject Dot-ELISA; ELISA; IgG; immunoassay; protein A; saliva; toxoplasma; toxoplasmosis
dc.subject.other antibodies; serum
dc.title Saliva as a source of anti-Toxoplasma gondii IgG for enzyme immunoassay in human samples
dc.type article
dc.rights.holder Copyright WILEY-BLACKWELL
dc.description.group LIM/49
dc.identifier.doi 10.1111/1469-0691.12295
dc.identifier.pmid 23848317
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author MEIRELES, L. R.:IMT:IMT
hcfmusp.author ANDRADE JR., H. F.:FM:MPT
hcfmusp.author.external · SAMPAIO, B. F. C.:Inst Med Trop Sao Paulo, Lab Protozool, BR-05403000 Sao Paulo, Brazil
· MACRE, M. S.:Univ Sao Paulo, Inst Ciencias Biomed, BR-05508 Sao Paulo, Brazil
hcfmusp.origem.id WOS:000328530900027
hcfmusp.origem.id 2-s2.0-84890787415
hcfmusp.publisher.city HOBOKEN
hcfmusp.publisher.country USA
hcfmusp.relation.reference · Cavalcante GT, 2006, J PARASITOL, V92, P647, DOI 10.1645/GE-774R.1
· FELDMAN M, 1990, T ROY SOC TROP MED H, V84, P559, DOI 10.1016/0035-9203(90)90040-L
· HAECKEL R, 1989, Journal of Clinical Chemistry and Clinical Biochemistry, V27, P246
· HAJEER AH, 1994, PARASITE IMMUNOL, V16, P43, DOI 10.1111/j.1365-3024.1994.tb00303.x
· JEGANATHAN S, 1987, CLIN EXP IMMUNOL, V70, P316
· Kaufman E, 2002, CRIT REV ORAL BIOL M, V13, P197
· Leal FE, 2007, CLIN INFECT DIS, V44, pE62, DOI 10.1086/511871
· Macre MD, 2009, REV INST MED TROP SP, V51, P283, DOI 10.1590/S0036-46652009000500009
· Morris M, 2002, J IMMUNOL METHODS, V266, P111, DOI 10.1016/S0022-1759(02)00114-X
· PERRY KR, 1993, J MED VIROL, V40, P235, DOI 10.1002/jmv.1890400312
· Shukla Nitin, 2008, Indian J Med Sci, V62, P222
· Stekler JD, 2009, CLIN INFECT DIS, V49, P444, DOI 10.1086/600043
· Wang ZJ, 2002, CHINESE MED J-PEKING, V115, P1460
· Wong D T W, 2011, Adv Dent Res, V23, P350, DOI 10.1177/0022034511420438
· Zarkovic A, 2007, CLIN EXP OPHTHALMOL, V35, P131, DOI 10.1111/j.1442-9071.2006.01406.x
dc.description.index MEDLINE
dc.identifier.eissn 1469-0691
hcfmusp.citation.scopus 6
hcfmusp.citation.wos 7
hcfmusp.affiliation.country Brasil


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