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https://observatorio.fm.usp.br/handle/OPI/7637
Title: | Recombinant Leishmania infantum Heat Shock Protein 83 for the Serodiagnosis of Cutaneous, Mucosal, and Visceral Leishmaniases |
Authors: | CELESTE, Beatriz Julieta; SANCHEZ, Maria Carmen Arroyo; RAMOS-SANCHEZ, Eduardo Milton; CASTRO, Luiz Guilherme M.; COSTA, Francisco Assis Lima; GOTO, Hiro |
Citation: | AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, v.90, n.5, p.860-865, 2014 |
Abstract: | Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. major-like when considering either CL/ML (P = 0.041) or all leishmaniasis patients (P = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA-L. major-like, specifically for Chagas disease samples (P < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML (P = 0.607) or CL (P = 0.205). We recommend ELISA-L. infantum-rHsp83 as a routine confirmatory serological assay for the diagnosis of Leishmania infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPR Artigos e Materiais de Revistas Científicas - LIM/38 |
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art_CELESTE_Recombinant_Leishmania_infantum_Heat_Shock_Protein_83_for_2014.PDF | publishedVersion (English) | 493.4 kB | Adobe PDF | View/Open |
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