Dynamic expression of desmin, alpha-SMA and TGF-beta 1 during hepatic fibrogenesis induced by selective bile duct ligation in young rats

Imagem de Miniatura
Arquivos
art_GONCALVES_Dynamic_expression_of_desmin_alphaSMA_and_TGFbeta_1_2014.PDF (813.89 KB)
Citações na Scopus
19
Tipo de produção
article
Data de publicação
2014
Editora
ASSOC BRAS DIVULG CIENTIFICA
DOI
Indexadores
SciELO
Scopus
Web of Science
Título da Revista
ISSN da Revista
Título do Volume
Autores
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.47, n.10, p.850-857, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
We previously described a selective bile duct ligation model to elucidate the process of hepatic fibrogenesis in children with biliary atresia or intrahepatic biliary stenosis. Using this model, we identified changes in the expression of alpha smooth muscle actin (alpha-SMA) both in the obstructed parenchyma and in the hepatic parenchyma adjacent to the obstruction. However, the expression profiles of desmin and TGF-beta 1, molecules known to be involved in hepatic fibrogenesis, were unchanged when analyzed by semiquantitative polymerase chain reaction (RT-PCR). Thus, the molecular mechanisms involved in the modulation of liver fibrosis in this experimental model are not fully understood. This study aimed to evaluate the molecular changes in an experimental model of selective bile duct ligation and to compare the gene expression changes observed in RT-PCR and in real-time quantitative PCR (qRT-PCR). Twenty-eight Wistar rats of both sexes and weaning age (21-23 days old) were used. The rats were separated into groups that were assessed 7 or 60 days after selective biliary duct ligation. The expression of desmin, alpha-SMA and TGF-beta 1 was examined in tissue from hepatic parenchyma with biliary obstruction (BO) and in hepatic parenchyma without biliary obstruction (WBO), using RT-PCR and qRT-PCR. The results obtained in this study using these two methods were significantly different. The BO parenchyma had a more severe fibrogenic reaction, with increased alpha-SMA and TGF-beta 1 expression after 7 days. The WBO parenchyma presented a later, fibrotic response, with increased desmin expression 7 days after surgery and increased alpha-SMA 60 days after surgery. The qRT-PCR technique was more sensitive to expression changes than the semiquantitative method.
Palavras-chave
Alpha smooth muscle actin, Desmin, Hepatic fibrogenesis, Selective bile duct ligation, RT-PCR, TGF-beta 1
URI
https://observatorio.fm.usp.br/handle/OPI/8278
Referências
  1. BACHEM MG, 1992, J CLIN INVEST, V89, P19, DOI 10.1172/JCI115561
  2. BALLARDINI G, 1994, HEPATOLOGY, V19, P440
  3. BALLARDINI G, 1988, VIRCHOWS ARCH B, V56, P45, DOI 10.1007/BF02890000
  4. Chobert MN, 2012, LAB INVEST, V92, P135, DOI 10.1038/labinvest.2011.143
  5. Dagher H, 2004, J VIROL METHODS, V117, P113, DOI 10.1016/j.jviromet.2004.01.003
  6. Dooley S, 2000, HEPATOLOGY, V31, P1094, DOI 10.1053/he.2000.6126
  7. Gaudio E, 2006, GASTROENTEROLOGY, V130, P1270, DOI 10.1053/j.gastro.2005.12.034
  8. Gibelli NEM, 2008, CLINICS, V63, P689, DOI 10.1590/S1807-59322008000500019
  9. Glaser S, 2008, AM J PHYSIOL-GASTR L, V295, pG124, DOI 10.1152/ajpgi.00536.2007
  10. Golbar HM, 2011, TOXICOL PATHOL, V39, P795, DOI 10.1177/0192623311413790
  11. Gressner A M, 1996, Kidney Int Suppl, V54, pS39
  12. Henderson NC, 2013, BBA-MOL BASIS DIS, V1832, P891, DOI 10.1016/j.bbadis.2012.10.005
  13. Iredale JP, 2013, BBA-MOL BASIS DIS, V1832, P876, DOI 10.1016/j.bbadis.2012.11.002
  14. JAKOWLEW SB, 1991, CELL REGUL, V2, P535
  15. Kaimori A, 2007, J BIOL CHEM, V282, P22089, DOI 10.1074/jbc.M700998200
  16. Karakayali F, 2013, TRANSPL P, V45, P3524, DOI 10.1016/j.transproceed.2013.09.012
  17. Lee UE, 2011, BEST PRACT RES CL GA, V25, P195, DOI 10.1016/j.bpg.2011.02.005
  18. Lewindon PJ, 2002, AM J PATHOL, V160, P1705, DOI 10.1016/S0002-9440(10)61117-0
  19. Lijnen P, 2002, METHOD FIND EXP CLIN, V24, P333, DOI 10.1358/mf.2002.24.6.693065
  20. Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
  21. Marzioni M, 2005, GASTROENTEROLOGY, V128, P121, DOI 10.1053/j.gastro.2004.10.002
  22. Meindl-Beinker NM, 2008, J GASTROEN HEPATOL, V23, pS122, DOI 10.1111/j.1440-1746.2007.05297.x
  23. Novo E, 2009, INT J BIOCHEM CELL B, V41, P2089, DOI 10.1016/j.biocel.2009.03.010
  24. Rosenbloom J, 2013, BBA-MOL BASIS DIS, V1832, P1088, DOI 10.1016/j.bbadis.2012.12.007
  25. Schmittgen TD, 2000, ANAL BIOCHEM, V285, P194, DOI 10.1006/abio.2000.4753
  26. Schrum LW, 2001, AM J PHYSIOL-GASTR L, V280, pG139
  27. TAKASE S, 1988, J HEPATOL, V6, P267, DOI 10.1016/S0168-8278(88)80042-4
  28. Tannuri ACA, 2012, J PEDIATR SURG, V47, P513, DOI 10.1016/j.jpedsurg.2011.10.009
  29. Tannuri U, 2004, TRANSPLANT P, V36, P941, DOI 10.1016/j.transproceed.2004.03.101
  30. Wadhawan M, 2013, J GASTROEN HEPATOL, V28, P1056, DOI 10.1111/jgh.12169

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCG
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/ICr
Artigos e Materiais de Revistas Científicas - LIM/26
Artigos e Materiais de Revistas Científicas - LIM/30