Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author ESPIRITO-SANTO, Maria Cristina Carvalho FMUSP-HC
ALVARADO-MORA, Monica Viviana
DIAS-NETO, Emmanuel FMUSP-HC
BOTELHO-LIMA, Livia Souza FMUSP-HC
MOREIRA, Joao Paulo FMUSP-HC
AMORIM, Maria
PINTO, Pedro Luiz Silva
HEATH, Ashley R.
CASTILHO, Vera Lucia Pagliusi FMUSP-HC
GONCALVES, Elenice Messias do Nascimento FMUSP-HC
LUNA, Expedito Jose de Albuquerque FMUSP-HC
CARRILHO, Flair Jose FMUSP-HC
PINHO, Joao Renato Rebello FMUSP-HC
GRYSCHEK, Ronaldo Cesar Borges FMUSP-HC
dc.date.issued 2014
dc.identifier.citation BMC INFECTIOUS DISEASES, v.14, article ID 558, 10p, 2014
dc.identifier.issn 1471-2334
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/8845
dc.description.abstract Background: Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato-Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE. Methods: A cross-sectional study conducted from April to December 2011 using a probabilistic sampling that collected 572 fecal and serum samples. The laboratory diagnostic techniques used were: KK, HH and qPCR ( feces and serum). Results: We obtained the following results using the different diagnostic techniques: KK and HH, 0.9% (n = 5); qPCR-feces, 9.6% (n = 55); and qPCR-serum, 1.4% (n = 8). The qPCR-feces presented the highest positivity, whereas the techniques of HH and KK were the least sensitive to detect infections (0.8%). Compared to HH and KK, qPCR-feces showed a statistically significant difference in positivity (p < 0.05), although with poor agreement. Conclusion: The positivity rate presented by the qPCR approach was far higher than that obtained by parasitological techniques. The lack of adequate surveillance in ALE of schistosomiasis indicates a high possibility of these areas being actually of medium and high endemicity. This study presents a control perspective, pointing to the possibility of using combined laboratory tools in the diagnosis of schistosomiasis in ALE.
dc.description.sponsorship · Sao Paulo Research Foundation [07/53457-7, 08/50461-6, 10/52615-0]
dc.language.iso eng
dc.publisher BIOMED CENTRAL LTD
dc.relation.ispartof BMC Infectious Diseases
dc.rights openAccess
dc.subject Schistosomiasis mansoni; TaqMan (R) Real-Time PCR system; Laboratory diagnosis; Low endemicity areas; Brazil/epidemiology
dc.subject.other polymerase-chain-reaction; ribosomal-rna genes; kato-katz; stool samples; low transmission; dna; diagnosis; intensity; areas; sensitivity
dc.title Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting
dc.type article
dc.rights.holder Copyright BIOMED CENTRAL LTD
dc.description.group LIM/06
dc.description.group LIM/27
dc.description.group LIM/03
dc.description.group LIM/07
dc.description.group LIM/52
dc.identifier.doi 10.1186/s12879-014-0558-4
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author ESPIRITO-SANTO, Maria Cristina Carvalho:HC:LIM/06
hcfmusp.author DIAS-NETO, Emmanuel:HC:LIM/27
hcfmusp.author BOTELHO-LIMA, Livia Souza:FM:
hcfmusp.author MOREIRA, Joao Paulo:FM:
hcfmusp.author CASTILHO, Vera Lucia Pagliusi:HC:ICHC
hcfmusp.author GONCALVES, Elenice Messias do Nascimento:HC:LIM/03
hcfmusp.author LUNA, Expedito Jose de Albuquerque:IMT:IMT
hcfmusp.author CARRILHO, Flair Jose:FM:MGT
hcfmusp.author PINHO, Joao Renato Rebello:HC:LIM/07
hcfmusp.author GRYSCHEK, Ronaldo Cesar Borges:FM:MIP
hcfmusp.author.external · ALVARADO-MORA, Monica Viviana:Univ Sao Paulo, Sch Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil
· AMORIM, Maria:AC Camargo Canc Ctr, Lab Med Genom, Sao Paulo, Brazil
· PINTO, Pedro Luiz Silva:Adolfo Lutz Inst, Parasitol & Mycol Serv, Dept Enteroparasites, Sao Paulo, Brazil
· HEATH, Ashley R.:Sigma Custom Prod, The Woodlands, TX 77380 USA
hcfmusp.origem.id WOS:000343831200001
hcfmusp.origem.id 2-s2.0-84929507684
hcfmusp.publisher.city LONDON
hcfmusp.publisher.country ENGLAND
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dc.description.index MEDLINE
hcfmusp.citation.scopus 16
hcfmusp.citation.wos 14
hcfmusp.affiliation.country Brasil
hcfmusp.affiliation.country Estados Unidos


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