Antitumour Efficacy of Piper tuberculatum and Piplartine Based on the Hollow Fiber Assay
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Citações na Scopus
23
Tipo de produção
article
Data de publicação
2015
Editora
GEORG THIEME VERLAG KG
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
BEZERRA, Daniel P.
FERREIRA, Paulo Michel P.
AQUINO, Nayara C. De
SILVEIRA, Edilberto R.
PESSOA, Claudia
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
PLANTA MEDICA, v.81, n.1, p.15-19, 2015
Resumo
Piper tuberculatum, popularly known in Brazil as ""jaborandi falso"" and ""pimenta darta"", is widely used in folk medicine for the treatment of several diseases. In this study, the in vivo hollow fiber assay was used to investigate the antitumour efficacy of the crude extract and piplartine obtained from P. tuberculatum roots. Human glioblastoma (SF-295) and colon carcinoma (HCT-8) cell lines were used. In vitro cytotoxicity was assayed by the MTT assay. In the hollow fiber assay, nude mice implanted with tumour cells in hollow fibers were treated for four consecutive days via the intraperitoneal route, and tumour cell populations were assessed by the MTT assay. Both the crude extract and piplartine displayed cytotoxicity. In the hollow fiber assay, tumour growth inhibition rates were 24.6-54.8% for the crude extract and 33.7-62.2% for piplartine. No signal of toxicity was noticed. In conclusion, the crude extract and piplartine obtained from P. tuberculatum roots displayed in vitro and in vivo anticancer efficacy.
Palavras-chave
Piper tuberculatum, Piperaceae, Piplartine, antitumour, hollow fiber
Referências
- Bezerra DP, 2007, TOXICOL IN VITRO, V21, P1, DOI 10.1016/j.tiv.2006.07.007
- Bezerra DP, 2008, J APPL TOXICOL, V28, P156, DOI 10.1002/jat.1261
- Bezerra DP, 2005, Z NATURFORSCH C, V60, P539
- Bezerra DP, 2006, BRAZ J MED BIOL RES, V39, P801, DOI 10.1590/S0100-879X2006000600014
- Bezerra DP, 2013, EUR J PHARM SCI, V48, P453, DOI 10.1016/j.ejps.2012.12.003
- Bezerra DP, 2008, MUTAT RES-GEN TOX EN, V652, P164, DOI 10.1016/j.mrgentox.2008.02.001
- Bezerra DP, 2011, QUIM NOVA, V35, P460
- Bezerra DP, 2009, MUTAT RES-GEN TOX EN, V677, P8, DOI 10.1016/j.mrgentox.2009.04.007
- Bodiwala HS, 2007, J NAT MED, V61, P418, DOI 10.1007/s11418-007-0159-2
- Burci LM, 2013, J ETHNOPHARMACOL, V148, P165, DOI 10.1016/j.jep.2013.04.006
- Chaves MCD, 2003, FITOTERAPIA, V74, P181
- Cotinguiba F, 2009, MED CHEM RES, V18, P703, DOI 10.1007/s00044-008-9161-9
- Diaz LE, 2012, MOLECULES, V17, P4142, DOI 10.3390/molecules17044142
- Felipe FCB, 2007, PHYTOMEDICINE, V14, P605, DOI 10.1016/j.phymed.2006.12.015
- HOLLINGSHEAD MG, 1995, LIFE SCI, V57, P131, DOI 10.1016/0024-3205(95)00254-4
- Jyothi D, 2009, TOXICOL IN VITRO, V23, P1085, DOI 10.1016/j.tiv.2009.05.023
- Kuete V, 2013, J ETHNOPHARMACOL, V149, P245, DOI 10.1016/j.jep.2013.06.029
- Lavor PL, 2012, NAT PROD COMMUN, V7, P1391
- MOSMANN T, 1983, J IMMUNOL METHODS, V65, P55, DOI 10.1016/0022-1759(83)90303-4
- Raj L, 2011, NATURE, V475, P231, DOI 10.1038/nature10167
- Rapado LN, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002251
- Suffiness M., 1990, METHODS PLANT BIOCH, P71
- Sunila ES, 2004, J ETHNOPHARMACOL, V90, P339, DOI 10.1016/j.jep.2003.10.016
- Taylor P, 2013, PHYTOTHER RES, V27, P530, DOI 10.1002/ptr.4752