In vitro mucus transportability, cytogenotoxicity, and hematological changes as non-destructive physiological biomarkers in fish chronically exposed to metals

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art_SERIANI_In_vitro_mucus_transportability_cytogenotoxicity_and_hematological_changes_2015.PDF (697.91 KB)
Citações na Scopus
32
Tipo de produção
article
Data de publicação
2015
Editora
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
ABESSA, Denis M. S.
MOREIRA, Lucas B.
CABRERA, Joana P. G.
SANCHES, Juliana Q.
SILVA, Carolina L. S.
AMORIM, Francisca A.
SILVA, Flavia L.
FITORRA, Lilian S.
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v.112, p.162-168, 2015
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The biomonitoring of fish using biomarkers represents a useful tool for the assessment of aquatic pollution. This study evaluated the sublethal toxic effects of aquatic pollution on fish collected from a site contaminated by metals. Water and fish (Oreochromis niloticus) samples were collected from a pond in the Parque Ecologico do Tiete (PET) that lies along the Tiete River (Sao Paulo, Brazil), and from a control site (an experimental fish farm). The metal content of the water was evaluated, and fish were used to examine the properties of gill mucus and blood. The PET fish were evaluated for alterations in the in vitro transportability of mucus and changes in blood properties (e.g., cell volume, hemoglobin concentration, red blood cells, and white blood cell count). The results of the water analyzes indicated metal levels above the legal standards for Fe (0.71 mg/L), Ni (0.06 mg/L), Mn (0.11 mg/L), and Pb (0.48 mg/L). Compared to the controls, the hematologic parameter analyzes of PET fish revealed significantly higher numbers of erythrocytes (RBC), leukocytes (WBC), lymphocytes, erythroblasts, and Mean Corpuscular Volume (MCV); however, the hemoglobin content and Mean Corpuscular Hemoglobin Concentration (MCHC) values were significantly lower. The frequencies of nuclear abnormalities and micronuclei were significantly higher and the mucociliary transport was significantly lower in PET fish than in the controls. These results suggest that fish from the contaminated site exhibit a series of physiological responses, which probably indicate health disturbances. Furthermore, the results suggest that blood and mucus are promising, non-destructive targets for use in the monitoring of pollution.
Palavras-chave
Biomarkers, Chronic contamination, Cyto-genotoxicity, Hematology, Metals, Mucus
URI
https://observatorio.fm.usp.br/handle/OPI/9080
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/59
Artigos e Materiais de Revistas Científicas - ODS/03
Artigos e Materiais de Revistas Científicas - ODS/14