Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats

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art_FRIAS NETO_Effects_of_ischemic_preconditioning_and_cilostazol_on_muscle_2014.PDF (340.37 KB)
Citações na Scopus
17
Tipo de produção
article
Data de publicação
2014
Editora
ACTA CIRURGICA BRASILEIRA
DOI
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SciELO
Scopus
Web of Science
PubMed
Título da Revista
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Título do Volume
Autores
FRIAS NETO, Carlos Alberto da Silva
SAAD, Karen Ruggeri
SAAD, Paulo Fernandes
Autor de Grupo de pesquisa
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Citação
ACTA CIRURGICA BRASILEIRA, v.29, suppl.3, p.17-21, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
PURPOSE: To evaluate effects of ischemic preconditioning and Cilostazol on muscle ischemia-reperfusion injury. METHODS: Male Wistar rats were submitted to muscle ischemic and reperfusion injury (4h of the left common iliac artery occlusion followed by 1h of reperfusion). Five experimental groups were constituted: Control group (n=4); Ischemia-Reperfusion (IR, n=5); Ischemic preconditioning group (IP, n=6); Ischemia-Reperfusion group treated with cilostazol (IRCi, n=6) and Ischemic preconditioning group treated with cilostazol (IPCi, n=6). At the end, left gracile muscle was removed and embedded in paraffin. Histopathology, neutrophil infiltration, myocyte necrosis and edema were analyzed. RESULTS: When compared with the control group, IR group showed increased neutrophil infiltration, severe necrosis and edema. There was significant difference between myocytes necrosis of IR group and IP group. There was no difference between the histopathological changes between IP, IRCi and IPCi groups. CONCLUSIONS: The model of IR caused severe muscle injury in the rat hind limb and ischemic preconditioning has a protective effect, reducing myocyte necrosis, however, treatment with cilostazol and also the association between cilostazol and preconditioning has no protective effect on the skeletal muscle subjected to ischemia and reperfusion injury.
Palavras-chave
Ischemic preconditioning, Muscle injury, Cilostazol, Rats
URI
https://observatorio.fm.usp.br/handle/OPI/9238
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCG
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - LIM/51
Artigos e Materiais de Revistas Científicas - LIM/62