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https://observatorio.fm.usp.br/handle/OPI/9266
Title: | N-acetylcysteine as a potential strategy to attenuate the oxidative stress induced by uremic serum in the vascular system |
Authors: | RODRIGUES, Silvia D.; FRANCA, Karime C.; DALLIN, Fernando T.; FUJIHARA, Clarice K.; NASCIMENTO, Aguinaldo J.; PECOITS-FILHO, Roberto; NAKAO, Lia S. |
Citation: | LIFE SCIENCES, v.121, p.110-116, 2015 |
Abstract: | Aims: Chronic kidney disease (CKD) progression is accompanied by systemic oxidative stress, which contributes to an increase in the risk of cardiovascular diseases (CVDs). N-acetylcysteine (NAC) is among the most studied antioxidants, but its therapeutic benefits in CKD-associated CVDs remain controversial. Here, we investigated whether NAC could inhibit the oxidative stress induced by uremia in vitro and in vivo. Main methods: Endothelial and smooth muscle cells were challenged with human uremic or non-uremic sera, and the effects of a pre-treatment with 2 mM NAC were evaluated. Reactive oxygen species (ROS) production, protein oxidation and total glutathione/glutathione disulfide (tGSH/GSSG) ratios were measured. Five-sixths nephrectomized or sham-operated rats were orally treated (in the drinking water) with 60 mg/kg/day NAC or not treated for 53 days. Plasma cysteine/cystine reduction potential E-h(Cyss/2Cys) was determined as a novel marker of the systemic oxidative stress. Key findings: NAC inhibited all the determined oxidative stress parameters, likely by increasing the tGSH/GSSG ratio, in both cell lines exposed to uremic serum. Orally administered NAC attenuated the systemic oxidative stress in uremic rats. Significance: The present results indicate that NAC, by preventing GSH depletion in vascular cells exposed to uremic serum and by attenuating the systemic oxidative stress during CKD progression, emerges as a potential strategy to prevent the oxidative stress induced by uremic toxicity in the vascular system. (C) 2014 Published by Elsevier Inc. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - LIM/16 Artigos e Materiais de Revistas Científicas - ODS/03 |
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