Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/9369
Title: CD8+T Lymphocyte Expansion, Proliferation and Activation in Dengue Fever
Authors: MATOS, Andreia Manso deCARVALHO, Karina InacioROSA, Daniela SantoroVILLAS-BOAS, Lucy SantosSILVA, Wanessa Cardoso daRODRIGUES, Celia Luiza de LimaOLIVEIRA, Olimpia Massae Nakasone Peel FurtadoLEVI, Jose EduardoARAUJO, Evaldo Stanislau AffonsoPANNUTI, Claudio SergioLUNA, Expedito Jose AlbuquerqueKALLAS, Esper George
Citation: PLOS NEGLECTED TROPICAL DISEASES, v.9, n.2, article ID e0003520, 14p, 2015
Abstract: Dengue fever induces a robust immune response, including massive T cell activation. The level of T cell activation may, however, be associated with more severe disease. In this study, we explored the level of CD8+ T lymphocyte activation in the first six days after onset of symptoms during a DENV2 outbreak in early 2010 on the coast of Sao Paulo State, Brazil. Using flow cytometry we detected a progressive increase in the percentage of CD8+ T cells in 74 dengue fever cases. Peripheral blood mononuclear cells from 30 cases were thawed and evaluated using expanded phenotyping. The expansion of the CD8+ T cells was coupled with increased Ki67 expression. Cell activation was observed later in the course of disease, as determined by the expression of the activation markers CD38 and HLA-DR. This increased CD8+ T lymphocyte activation was observed in all memory subsets, but was more pronounced in the effector memory subset, as defined by higher CD38 expression. Our results show that most CD8+ T cell subsets are expanded during DENV2 infection and that the effector memory subset is the predominantly affected sub population.
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Artigos e Materiais de Revistas Científicas - FM/MCM
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Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

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LIM/52 - Laboratório de Virologia

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