Simultaneous Quantification of Lycopene, beta-Carotene, Retinol and alpha-Tocopherol in Plasma after a Simple Extraction Procedure: Stability Study and Application to Human Volunteers

Show simple item record

dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author CHARAO, Mariele F.
MORO, Angela M.
BRUCKER, Natalia
BULCAO, Rachel P.
BAIERLE, Marilia
FREITAS, Fernando
DURGANTE, Juliano
NASCIMENTO, Sabrina
BUBOLS, Guilherme B.
SALDIVA, Paulo H. FMUSP-HC
BOHRERE, Denise
GARCIA, Solange C.
dc.date.issued 2012
dc.identifier.citation JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, v.23, n.8, p.1441-1449, 2012
dc.identifier.issn 0103-5053
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/942
dc.description.abstract A method for the simultaneous quantification of lycopene, beta-carotene, retinol and alpha-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20 degrees C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra-and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20 degrees C. A significant decrease of lycopene, beta-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.
dc.description.abstract Um método para quantificação simultânea de licopeno, β-caroteno, retinol e α-tocoferol por cromatografia líquida de alta eficiência (HPLC) com detecção no visível/fluorescente e eluição isocrática foi otimizado e validado. O método consiste de extração líquido-líquido rápida e simples e posterior quantificação do sobrenadante extraído por HPLC. Alíquotas de plasma foram estocadas a –20°C por três meses para estudo da estabilidade. Aplicação metodológica foi realizada em amostras fornecidas por pintores e indivíduos não expostos a tintas (n = 75). O ensaio foi linear para todas as vitaminas analisadas (r > 0,99). Precisões intradia e interdia apresentaram coeficiente de variação (CV) menor que 5%. Exatidões variaram de 0,29 a –5,80% e recuperações entre 92,73 e 101,97%. Amostras de plasma e sobrenadante extraído foram estáveis por até 60 dias a –20°C. Foi demonstrada uma diminuição significativa nas concentrações de licopeno, β-caroteno e retinol em indivíduos expostos quando comparados com os não-expostos (p < 0,05). O método é simples, reprodutível, preciso, exato e sensível, e pode ser utilizado na rotina de laboratórios clínicos.
dc.description.sponsorship · Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [CAPES/DAAD PROBRAL 352/10]
· Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq/MCT) [479613/2009-5]
· CAPES/REUNI
· CNPq
dc.language.iso eng
dc.publisher SOC BRASILEIRA QUIMICA
dc.relation.ispartof Journal of the Brazilian Chemical Society
dc.rights openAccess
dc.subject retinol; alpha-tocopherol; lycopene; beta-carotene; HPLC; liquid-liquid extraction
dc.subject.other performance liquid-chromatography; human serum; electrochemical detection; vitamin-a; validation; antioxidants; standard
dc.title Simultaneous Quantification of Lycopene, beta-Carotene, Retinol and alpha-Tocopherol in Plasma after a Simple Extraction Procedure: Stability Study and Application to Human Volunteers
dc.type article
dc.rights.holder Copyright SOC BRASILEIRA QUIMICA
dc.description.group LIM/05
dc.identifier.doi 10.1590/S0103-50532012005000001
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author SALDIVA, Paulo H.:FM:MPT
hcfmusp.author.external · CHARAO, Mariele F.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Santa Maria, Ctr Hlth Sci, Postgrad Program Pharmacol, BR-97105900 Santa Maria, RS, Brazil
· MORO, Angela M.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
· BRUCKER, Natalia:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
· BULCAO, Rachel P.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
· BAIERLE, Marilia:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
· FREITAS, Fernando:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
· DURGANTE, Juliano:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil
· NASCIMENTO, Sabrina:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil
· BUBOLS, Guilherme B.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
· BOHRERE, Denise:Univ Fed Santa Maria, Dept Chem, BR-97105900 Santa Maria, RS, Brazil
· GARCIA, Solange C.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.origem.id WOS:000309162200006
hcfmusp.origem.id 2-s2.0-84866407917
hcfmusp.origem.id SCIELO:S0103-50532012000800006
hcfmusp.publisher.city SAO PAULO
hcfmusp.publisher.country BRAZIL
hcfmusp.relation.reference · Abahusain MA, 1998, BIOMED CHROMATOGR, V12, P89, DOI 10.1002/(SICI)1099-0801(199803/04)12:2<89::AID-BMC732>3.3.CO;2-S
· Aust O, 2001, J CHROMATOGR A, V936, P83, DOI 10.1016/S0021-9673(01)01269-9
· Boonsiri P, 2007, ASIA PAC J CLIN NUTR, V16, P47
· Burtis C., 1998, FUNDAMENTOS QUIMICA
· Casal S, 2001, J CHROMATOGR B, V763, P1, DOI 10.1016/S0378-4347(01)00349-8
· Causon R, 1997, J CHROMATOGR B, V689, P175, DOI 10.1016/S0378-4347(96)00297-6
· COMSTOCK GW, 1995, CANCER EPIDEM BIOMAR, V4, P505
· CRAFT NE, 1992, METHOD ENZYMOL, V213, P185
· Finckh B, 1995, ANAL BIOCHEM, V232, P210, DOI 10.1006/abio.1995.0009
· Lee BL, 2003, CLIN CHEM, V49, P2056, DOI 10.1373/clinchem.2003.022681
· MACCREHAN WA, 1987, CLIN CHEM, V33, P1585
· Olmedilla B, 2001, BRIT J NUTR, V85, P227, DOI 10.1079/BJN2000248
· Ortega H, 2004, J CHROMATOGR B, V803, P249, DOI 10.1016/j.jchromb.2003.12.025
· Ruperez FJ, 2004, J CHROMATOGR B, V800, P225, DOI 10.1016/j.jchromb.2003.10.016
· Semeraro A, 2009, J CHROMATOGR B, V877, P1209, DOI 10.1016/j.jchromb.2009.02.063
· Shi J, 2004, BIOFACTORS, V21, P203
· Siluk D, 2007, J PHARMACEUT BIOMED, V44, P1001, DOI 10.1016/j.jpba.2007.03.033
· Stahl W, 2005, BBA-MOL BASIS DIS, V1740, P101, DOI 10.1016/j.bbadis.2004.12.006
· Steghens JP, 1997, J CHROMATOGR B, V694, P71, DOI 10.1016/S0378-4347(97)00140-0
· Su Q, 1999, J CHROMATOGR B, V729, P191, DOI 10.1016/S0378-4347(99)00162-0
· Taibi G, 2002, J CHROMATOGR B, V780, P261, DOI 10.1016/S1570-0232(02)00529-9
· Talwar D, 1998, CLIN CHIM ACTA, V270, P85, DOI 10.1016/S0009-8981(97)00224-6
· Thibeault D, 2009, J CHROMATOGR B, V877, P1077, DOI 10.1016/j.jchromb.2009.02.051
· Thomas JB, 2001, CLIN CHIM ACTA, V305, P141
· Tzouganaki ZD, 2002, ANAL CHIM ACTA, V467, P115, DOI 10.1016/S0003-2670(02)00304-5
· VECCHI M, 1973, J CHROMATOGR, V83, P447, DOI 10.1016/S0021-9673(00)97061-4
· Vertzoni MV, 2005, J CHROMATOGR B, V819, P149, DOI 10.1016/j.jchromb.2005.02.001
· Wang LH, 2001, J PHARMACEUT BIOMED, V25, P785, DOI 10.1016/S0731-7085(01)00381-8
· Xu F, 2006, J CHROMATOGR B, V838, P44, DOI 10.1016/j.jchromb.2006.04.004
· Yuan JM, 2006, J NATL CANCER I, V98, P482, DOI 10.1093/jnci/djj104
· [Anonymous], 2005, Q2R1 ICH, P1
dc.description.index PubMed
hcfmusp.citation.scopus 7
hcfmusp.citation.wos 7
hcfmusp.affiliation.country Brasil


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace



Browse

My Account

Statistics