Seroepidemiological analysis of toxoplasmosis in college students

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art_RODRIGUES_Seroepidemiological_analysis_of_toxoplasmosis_in_college_students_2015.PDF (372.49 KB)
Citações na Scopus
9
Tipo de produção
article
Data de publicação
2015
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
BIOMED CENTRAL LTD
Autores
FREI, Fernando
NAVARRO, Italmar Teodorico
SILVA, Luciana Pereira
MARCELINO, Monica Yonashiro
FARIA, Carolina Arruda de
SANTOS, Marislene
RIBEIRO-PAES, Joao Tadeu
Citação
JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES, v.21, article ID 1, 7p, 2015
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods: In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondii antibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results: The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion: In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.
Palavras-chave
Toxoplasma gondii, Toxoplasmosis, Enzyme-linked immunosorbent assay, Indirect fluorescent antibody test, Seroprevalence, Epidemiology
URI
https://observatorio.fm.usp.br/handle/OPI/9478
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/48
Artigos e Materiais de Revistas Científicas - LIM/49