JORGE ELIAS KALIL FILHO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
36 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 36
- Sensitization to cat allergen and its association with respiratory allergies: cross-sectional study(2017) GALVAO, Clovis Eduardo Santos; GRAUDENZ, Gustavo Silveira; KALIL, Jorge; CASTRO, Fabio Fernandes MoratoCats are a significant source of allergens that contribute towards worsening of allergic diseases. The aim of this study was to investigate the association between sensitization to cat allergens and allergic respiratory diseases. This was an observational retrospective study based on the skin prick tests results of patients at a tertiary-level hospital in Sao Paulo. A total of 1,985 test results were assessed. The prevalence of sensitization to cat allergen was 20% (399 patients). Our data indicated that in this population of atopic patients, a positive skin prick test result for cat allergen was not associated significantly with a diagnosis of respiratory allergy.
- IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy(2023) FIGO, Daniele Danella; MACEDO, Priscilla Rios Cordeiro; GADERMAIER, Gabriele; REMUZGO, Cesar; CASTRO, Fabio Fernandes Morato; KALIL, Jorge; GALVAO, Clovis Eduardo Santos; SANTOS, Keity SouzaSublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific immunotherapy with peptide vaccines is used far less, but it is of great interest in the treatment of allergic reactions, as it precludes the drawbacks of allergen extracts. The ideal peptide candidates would bind to IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences of main allergens, Der p 1, 2, 5, 7, 10, 23 and Blo t 5, 6, 12, 13, were included in a 15-mer peptide microarray and tested against pooled sera from 10 patients pre- and post-1-year SLIT. All allergens were recognized to some extent by at least one antibody isotype and peptide diversity was higher post-1-year SLIT for both antibodies. IgE recognition diversity varied among allergens and timepoints without a clear tendency. Der p 10, a minor allergen in temperate regions, was the molecule with more IgE-peptides and might be a major allergen in populations highly exposed to helminths and cockroaches, such as Brazil. SLIT-induced IgG4 epitopes were directed against several, but not all, IgE-binding regions. We selected a set of peptides that recognized only IgG4 or were able to induce increased ratios of IgG4:IgE after one year of treatment and might be potential targets for vaccines.
bookPart Medicina de precisão em alergia(2022) SANTOS, Keity Souza; LIMA, Karine de Amicis; CASTRO, Fábio Fernandes Morato; KALIL, JorgeconferenceObject Clinical evolution of Kaposi's varicelliform eruption using Immunosuppressive drugs as treatment for atopic dermatitis(2020) PACHANI, M. A. D. S.; ZANETTI, F. A.; BIALOWAS, D.; NUNES, S. A.; LOPES, M. M.; MARTINS, C. R. S.; CASTRO, F. F. M.; KALIL, J.; YANG, A. C.- Eosinophilic Esophagitis: Latent Disease in Patients with Anaphylactic Reaction to Cow's Milk(2018) BARBOSA, Adriana C.; CASTRO, Fabio Morato; MEIRELES, Paula Rezende; ARRUDA, L. Karla; CARDOSO, Silvia Regina; KALIL, Jorge; YANG, Ariana C.BACKGROUND: Food allergy and eosinophilic esophagitis are a substantial and evolving public health issue. Clinicians should know the relationship between these diseases and how one may predispose to the other. This can help minimize misdiagnosis. OBJECTIVE: The objective of this study was to assess esophageal eosinophilia and eosinophilic esophagitis frequency in patients with persistent cow's milk allergy and anaphylaxis manifestations. METHODS: Patients with persistent cow's milk allergy with anaphylaxis manifestations were enrolled from 2012 through 2016 at the Sao Paulo University Hospital, Brazil. All of them were submitted to endoscopy despite the presence or absence of gastrointestinal symptoms. Demographics data, atopic comorbidities, medication use, endoscopic findings, and esophageal eosinophilia frequency were evaluated. RESULTS: Eighty-nine patients were selected. The median age was 8 years. It was observed that 34 of 89 patients (38.2%; 95% confidence interval [CI]: 28.14%-49.16%) presented with esophageal eosinophilia. Five patients (7.1%) presented proton pump inhibitor-responsive esophageal eosinophilia, and 10 patients (14.2%) presented eosinophilic esophagitis. We found that 29.4% were asymptomatic patients, 23.5% had nonspecific symptoms, 23.5% had persistent typical symptoms, and 23.5% had intermittent typical symptoms. There was an association with inflammatory endoscopy findings in 21 patients (61.7%). CONCLUSIONS: This description demands scientific attention because it is the highest frequency of esophageal eosinophilia yet described in a group of patients with cow's milk allergy presenting with anaphylaxis. Eosinophilic esophagitis is a condition that can coexist ""silently"" with an IgE-mediated food allergy and is most often underestimated and under-diagnosed. (C) 2017 American Academy of Allergy, Asthma & Immunology
- Recessive NLRC4-Autoinflammatory Disease Reveals an Ulcerative Colitis Locus(2022) STEINER, Annemarie; REYGAERTS, Thomas; PONTILLO, Alessandra; CECCHERINI, Isabella; MOECKING, Jonas; MOGHADDAS, Fiona; DAVIDSON, Sophia; CAROLI, Francesco; GROSSI, Alice; CASTRO, Fabio Fernandes Morato; KALIL, Jorge; GOHR, Florian N.; I, Florian Schmidt; BARTOK, Eva; ZILLINGER, Thomas; HARTMANN, Gunther; GEYER, Matthias; GATTORNO, Marco; MENDONCA, Leonardo Oliveira; MASTERS, Seth L.Purpose NLRC4-associated autoinflammatory disease (NLRC4-AID) is an autosomal dominant condition presenting with a range of clinical manifestations which can include macrophage activation syndrome (MAS) and severe enterocolitis. We now report the first homozygous mutation in NLRC4 (c.478G > A, p.A160T) causing autoinflammatory disease with immune dysregulation and find that heterozygous carriers in the general population are at increased risk of developing ulcerative colitis. Methods Circulating immune cells and inflammatory markers were profiled and historical clinical data interrogated. DNA was extracted and sequenced using standard procedures. Inflammasome activation assays for ASC speck formation, pyroptosis, and IL-1 beta/IL-18 secretion confirmed pathogenicity of the mutation in vitro. Genome-wide association of NLRC4 (A160T) with ulcerative colitis was examined using data from the IBD exomes portal. Results A 60-year-old Brazilian female patient was evaluated for recurrent episodes of systemic inflammation from six months of age. Episodes were characterized by recurrent low-grade fever, chills, oral ulceration, uveitis, arthralgia, and abdominal pain, followed by diarrhea with mucus and variable skin rash. High doses of corticosteroids were somewhat effective in controlling disease and anti-IL-1 beta therapy partially controlled symptoms. While on treatment, serum IL-1 beta and IL-18 levels remained elevated. Genetic investigations identified a homozygous mutation in NLRC4 (A160T), inherited in a recessive fashion. Increased ASC speck formation and IL-1 beta/IL-18 secretion confirmed pathogenicity when NLRC4 (A160T) was analyzed in human cell lines. This allele is significantly enriched in patients with ulcerative colitis: OR 2.546 (95% 1.778-3.644), P = 0.01305. Conclusion NLRC4 (A160T) can either cause recessively inherited autoinflammation and immune dysregulation, or function as a heterozygous risk factor for the development of ulcerative colitis.
conferenceObject Systemic inflammatory syndromes with immunodysregulatory phenotype: clinical characteristics, genetic findings and therapeutic response(2021) PEREIRA, Grazielly Pereira; PRADO, Alex; BRANDAO, Jaqueline Brandao; ZANETTI, Francine Zanetti; ALBERTO, Nazoneth; ALBERTO, Samucanda; KALIL, Jorge; FONSECA, Luiz; CASTRO, Fabio Castro; TOLEDO-BARROS, Myrthes; MENDONCA, Leonardo Mendonca- Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex(2013) SANTOS, Keity Souza; GADERMAIER, Gabriele; VEJVAR, Eva; ARCURI, Helen Andrade; GALVAO, Clovis Eduardo; YANG, Ariana Campos; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; HIMLY, Martin; MARI, Adriano; LISO, Marina; POMPONI, Debora; BREITENEDER, Heimo; WAGNER, Stefan; KALIL, Jorge; FERREIRA, Fatima; CASTRO, Fabio Fernandes MoratoScope Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. Methods and results The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome. Conclusion Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.
conferenceObject Inflammatory cell response, functional and biochemical features of the airways of professional cleaning workers upon exposure in the workplace(2017) LIMA, Cynthia F. Mafra; SARAIVA, Beatriz M.; KALIL, Jorge; CASTRO, Fabio M.; GALVAO, Clovis Eduardo S.conferenceObject In vitro NLRP3 inflammasome activation assay assists diagnosis of genetically negative CAPS patients and guides anti-IL1 therapy(2020) MENDONCA, L. O.; TOLEDO-BARROS, M. A. M.; FRANCO, P. A.; GIAVINA-BIANCHI JUNIOR, P. F.; KALIL, J. E.; CASTRO, F. F. Morato; CAROLI, F.; GROSSI, A.; CECCHERINI, I; ROBAZZI, T.; PILEGGI, G. S.; RIVITTI, M. C.; SANCHES JUNIOR, J. A.; GRUMACH, A. S.; GATTORNO, M.; PONTILLO, A.