ANA PAULA PEREIRA VELOSA

Índice h a partir de 2011
12
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LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina

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Agora exibindo 1 - 10 de 58
  • conferenceObject
    Increased decorin and type V collagen in SSc pulmonary fibrosis
    (2012) TEODORO, W.; VELOSA, A. P.; MARCELINO, A.; MARTIN, P.; CARRASCO, S.; GOLDENSTEIN-SCHAINBERG, C.; PARRA, E.; YOSHINARI, N.; CAPELOZZI, V.
    Objective: To evaluate COL V and decorin expression in pulmonary tissue and to characterize biochemical profile of COLV from lung fibroblasts culture from SSc patients. Method: We evaluated COL V and decorin expression and tridimensional reconstruction (3D) of 6 patients with SSc without pulmonary hypertension that underwent surgical lung biopsy and as control was obtained lung fragments from 6 normal individuals who died from trauma. COL V amount in lung sections was evaluated with immunofluorescence. To biochemical characterization of COL V from lung fibroblasts culture was used quantitative immunoblot. Results: It was found that the structure of COLV fibers was distorted and strongly thickened in lung tissue from SSc patients compared with thin fibers pattern in the healthy controls. Decorin was distributed around COL V fibrils in the bronchovascular interstitium and vascular walls. Histomorphometric analysis of SSc lung demonstrated increased expression of both COL V and decorin when compared to the control (p<0.01). The semiquantitative imunoblot detected an increased high molecular weight COLV fraction in patients when compared to the control. Conclusion: The over expression and unusual organization of COLV fibers with biochemical changes associated to increased decorin indicates that matrix signalization pathway is involved in COLV fibrillogenesis process in SSc pulmonary fibrosis.
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    Pathophysiological features of lung and skin in human SSc and collagen V/C57LB6 mouse model
    (2018) TEODORO, W. Paganelli Rosolia; VELOSA, A. P. Pereira; QUEIROZ, Z. A. de Jesus; SANTOS, L. Araujo dos; CATANOZI, S.; SANTOS FILHO, A. dos; BUENO, C.; VENDRAMINI, M. Borges Galhardo; FERNEZLIAN, S. de Moraes; EHER, E. Miristene; LOPES, F. Degobbi T. Q. S.; CAPELOZZI, V. L.
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    Hyperacute Rejection in Multivisceral Xenotransplantation
    (2012) GALVAO, F.; SOLER, W.; POMPEU, E.; COSTA, A.; WAISBERG, D.; CAPELLOZI, V.; TEODORO, W.; VELOSA, A.; CHAIB, E.; D'ALBUQUERQUE, L.
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    COLLAGEN TYPE V FACILITATE THE DIFFERENTIATION OF RABBIT ADIPOSE TISSUE-DERIVED STEM CELLS INTO A CHONDROCYTE-LIKE PHENOTYPE ""IN VITRO""
    (2012) CRUZ, Isabele B.; GOLDENSTEIN-SCHAINBERG, C.; FULLER, R.; VELOSA, A. P.; CARRASCO, S.; CAPELOZZI, V.; YOSHINARI, N. H.; TEODORO, W. R.
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    Cigarette smoke exposure leads to bone resorption, matrix remodeling and a worsening in bone mineralization
    (2016) TEODORO, W. Rosolia; BARHOSA, A. Povoa; LOURENCO, J. Dias; VELOSA, A. P. Pereira; MARTINS, J.; OLIVO, C. Rosa; JORGETTI, V.; LOPES, F. D. T. Q. S.
  • conferenceObject
    Collagen V induces differentiation of rabbit adipose tissue-derived stem cells in chondrocyte-like phenotype
    (2012) TEODORO, W.; CRUZ, I. Brindo da; VELOSA, A. P.; CARRASCO, S.; GOLDENSTEIN-SCHAINBERG, C.; FULLER, R.; PARRA, E.; CAPELOZZI, V.
    Objective: Stimulated mesenchymal stem cells (MSCs) have capacity of differentiation in many cell types. It is being used in degenerative diseases treatment protocols. We evaluated the collagen V (COL V) and collagen XI (COL XI) influence in the differentiation of rabbits adipose tissue-derived MSCs in a chondrocyte-like cell phenotype. Method: MSCs isolated of New Zealand rabbits adipose-tissue were maintained in culture by 4 weeks. COLV, COLXI and COLV/XI (10μg/ml) were added to culture during 72 h. The cells aggregates were stained with Toluidine blue, Alcian blue and Picrosirius. Chondrocyte-like phenotype was confirmed by immunofluorescence to CD34, vimentin and collagens I, II and III. Results: MSCs stimulated with COLV expressed proteoglicans and collagen, when compared with COLXI and COLV/XI and control. In the presence of COLV, MSCs was capable to increase collagen II expression confirming its chondro-cyte-like cell phenotype. In contrast, MSCs cultured with COLXI and COLV/XI express collagen I and III. Conclusion: The data suggest that COLV may facilitate the differentiation of rabbit adipose tissue-derived stem cells into a chondrocyte-like phenotype. Further studies are urged in order to evaluate the influence of COLV in the ability of chondrocytes to remodel osteoarthritic joint surface at ultrastructural and molecular levels.
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    Unusual distribution of Type V collagen isoforms determines the cutaneous fibrosis in scleroderma
    (2016) TEODORO, W. Rosolia; MORAIS, J.; VELOSA, A. P. Pereira; MARTIN, P.; CARRASCO, S.; CAMARGO, L.; GOLDENSTEIN-SCHAINBERG, C.; CAPELOZZI, V.
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    Type V collagen induced pulmonary fibrosis in C57B1/6mice
    (2014) TEODORO, Walcy Rosolia; VELOSA, Ana Paula Pereira; SANTOS FILHO, Antonio dos; ANDRADE, Priscila Cristina; MARTINS, Vanessa; FERNEZLIAN, Sandra Morais; CATANOZI, Sergio; CAPELOZZI, Vera Luisa
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    Collagen V Maintains Experimental Pulmonary Fibrosis In Il17-dependent And -Independent Pathways
    (2013) FABRO, A. T.; SILVA, P. Q.; ZOCOLARO, W. S.; ALMEIDA, M. S.; MINATEL, I. O.; PRANDO, E. C.; RAINHO, C. A.; VELOSA, A. P.; TEODORO, W. R.; PARRA-CUENTAS, E. R.; POPPER, H. H.; CAPELOZZI, V. L.
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    Metalloproteases gene expression and remodeling of lung parenchyma fibers during the progression of elastase induced emphysema
    (2014) ROBERTONI, Fabiola Santos Zambon; OLIVO, Clarice Rosa; LOURENCO, Juliana Dias; GONCALVES, Natalia Comes; VELOSA, Ana Paula Pereira; TEODORO, Walcy Rosolia; LIN, Chin Jia; MARTINS, Milton De Arruda; LOPES, Fernanda D. T. Q. dos Santos