CAROLINE CRISTIANO REAL GREGORIO
Projetos de Pesquisa
Unidades Organizacionais
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Exercise protects synaptic density in a rat model of Parkinson's disease(2021) BINDA, K. H.; LILLETHORUP, T. P.; REAL, C. C.; BAERENTZEN, S. L.; NIELSEN, M. N.; ORLOWSKI, D.; BROOKS, D. J.; CHACUR, M.; LANDAU, A. M.Background: Parkinson's disease (PD) is characterized by Lewy body and neurite pathology associated with dopamine terminal dysfunction. Clinically, it is associated with motor slowing, rigidity, and tremor. Postural instability and pain are also features. Physical exercise benefits PD patients - possibly by promoting neuroplasticity including synaptic regeneration. Objectives: In a parkinsonian rat model, we test the hypotheses that exercise: (a) increases synaptic density and reduces neuroinflammation and (b) lowers the nociceptive threshold by increasing mu-opioid receptor expression. Methods: Brain autoradiography was performed on rats unilaterally injected with either 6-hydroxydopamine (6OHDA) or saline and subjected to treadmill exercise over 5 weeks. [3H]UCB-J was used to measure synaptic vesicle glycoprotein 2A (SV2A) density. Dopamine D2/3 receptor and mu-opioid receptor availability were assessed with [3H]Raclopride and [3H]DAMGO, respectively, while neuroinflammation was detected with the 18kDA translocator protein (TSPO) marker [3H]PK11195. The nociceptive threshold was determined prior to and throughout the exercise protocol. Results: We confirmed a dopaminegic deficit with increased striatal [3H]Raclopride D2/3 receptor availability and reduced nigral tyrosine hydroxylase immunoreactivity in the ipsilateral hemisphere of all 6-OHDA-injected rats. Sedentary rats lesioned with 6-OHDA showed significant reduction of ipsilateral striatal and substantia nigra [3H]UCB-J binding while [3H]PK11195 showed increased ipsilateral striatal neuroinflammation. Lesioned rats who exercised had higher levels of ipsilateral striatal [3H]UCB-J binding and lower levels of neuroinflammation compared to sedentary lesioned rats. Striatal 6-OHDA injections reduced thalamic mu-opioid receptor availability but subsequent exercise restored binding. Exercise also raised thalamic and hippocampal SV2A synaptic density in 6-OHDA lesioned rats, accompanied by a rise in nociceptive threshold. Conclusion: These data suggest that treadmill exercise protects nigral and striatal synaptic integrity in a rat lesion model of PD - possibly by promoting compensatory mechanisms. Exercise was also associated with reduced neuroinflammation post lesioning and altered opioid transmission resulting in an increased nociceptive threshold.
- Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors(2020) BINDA, K. H.; REAL, C. C.; FERREIRA, A. F. F.; BRITTO, L. R.; CHACUR, M.In addition to motor symptoms, Parkinson's disease (PD) presents high prevalence of painful symptoms responsible for worsening quality of life of PD patients. Physical exercise can improve such painful symptoms. This study evaluated the effects of exercise on nociceptive threshold using an unilateral rat model of PD, as well as the role played by cannabinoid and opioid receptors in areas responsible for pain pathways. For PD induction, Wistar rats were injected with 6-OHDA. 15?days after, rats either remained sedentary or were forced to exercise three times a week for 40 min. Motor and nociceptive behaviors were evaluated through cylinder and mechanical hyperalgesia tests, respectively. The animals were euthanized for analysis of cannabinoid receptor type 1 (CB1) and type 2 (CB2), and mu-opioid receptor (MOR) in the anterior cingulate cortex (ACC), periaqueductal gray matter (PAG), and thalamus areas by immunohistochemistry (IHC) and Western blotting. Our data revealed a decrease in the nociceptive threshold in both forepaws after surgery; in contrast, there was improvement in painful symptoms after the exercise protocol. For cannabinoid system there were an increase in CB2 expression in the ACC and PAG, and in CB1 levels in the PAG. And for opioid system there was an increase of MOR expression in the thalamus. Thus, modulation of those receptors by physical exercise can be an important non-pharmacological intervention to reduce painful symptoms in a rat model of PD, contributing to knowledge and promotion of better treatment aimed at improving the quality of life of PD patients.
- Exercise and Parkinson’s disease: Linking in the cannabinoid type 1 (CB1) and type 2 (CB2) and mu-opioid receptors(2023) CHACUR, M.; BINDA, K. H.; REAL, C. C.Parkinson’s disease (PD) has motor alterations as typical symptoms; however, there are also a high prevalence of nonmotor symptoms such as pain, balance, depression with high impact on patient quality life. Pain is present in around of 85% of PD patients and is associated with abnormal processing in central areas of pain pathway, and opioid and endocannabinoid system can be involved. Physical exercise has beneficial effects on PD, improving motor and nonmotor symptoms. This chapter aimed to highlight studies that report the effectiveness of the intervention through exercise on nonmotor symptoms of patients and animal models of PD, focusing on opioid and endocannabinoid system. In general, the exercise and physical activity can improve many PD symptoms; it helps maintain balance, mobility, and the ability to perform daily routines. That physical activity can play a preventive and maintenance role of physical fitness and mental health for patients with PD and consequently improve quality of life of PD patients. © 2023 Elsevier Inc. All rights reserved.
- Effects of physical exercise on mitochondrial biogenesis in a mouse model of Parkinson's disease(2019) FERREIRA, A. F. F.; BINDA, K. H.; REAL, C. C.; BRITTO, L. R. G.
conferenceObject [F-18] FDG PET imaging study in early phase of unilateral 6-OHDA Parkinson disease rats model submitted to treadmill exercise protocol(2017) REAL, C. C.; BINDA, K. H.; GARCIA, P. C.; CARNEIRO, C. G.; BUCHPIGUEL, C. A.; FARIA, D. P.; BRITTO, L. R. G.- Treadmill exercise and neuroinflammation: Links with aging(2021) REAL, C. C.; BINDA, K. H.; LANDAU, A. M.The lifestyle choices of modern society, such as lack of physical activity, overeating, and poor sleep habits can lead to neuroinflammation which is detrimental to a number of processes in the brain and has been linked to accelerated brain aging, and psychiatric and neurological diseases. Exercise protocols have been described as potential non-pharmacological interventions to protect against, or reverse, the adverse effects of lifestyle habits on the brain. In this chapter, we focus on previous evidence of the beneficial effects of treadmill exercise on reducing neuroinflammation and its links with the aging process and neurodegenerative diseases. © 2021 Elsevier Inc. All rights reserved.