PAULO MANUEL PEGO FERNANDES
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cardio-Pneumologia, Faculdade de Medicina - Docente
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica, Hospital das Clínicas, Faculdade de Medicina - Líder
28 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 28
- The 500th Lung Transplantation at the Faculdade de Medicina da Universidade de Sao Paulo: Reflecting on Our Journey and Looking Ahead(2023) REIS, Flavio Pola dos; PEGO-FERNANDES, Paulo Manuel
- Robotic surgery training(2023) ARAUJO, Pedro Henrique Xavier Nabuco de; PEGO-FERNANDES, Paulo Manuel
- Translational Medicine and Implementation Science(2023) LUZ, Protasio Lemos da; PEGO-FERNANDES, Paulo Manuel
conferenceObject The Use of Intraoperative Ecmo in Lung Transplantation: A Retrospective Analysis from the Largest Lung Transplant Center in Brazil(2023) SANTOS, S. dos; RAZUK FILHO, M.; POLA, F.; ABDALLA, L. G.; FERNANDES, L. M.; PEGO-FEMANDES, P.bookPart Ligadura de ducto torácico(2023) PêGO-FERNANDES, Paulo M.; MINAMOTO, Fabio Eiti Nishibe; BEYRUTI, RicardobookPart Ressecções de tumores invasivos de mediastino(2023) CURY, Caio Barbosa; ABDALLA, Luis Gustavo; PêGO-FERNANDES, Paulo M.; ARAúJO, Pedro Henrique Xavier Nabuco de- Effect of paclitaxel and methotrexate associated with cholesterol-rich nanoemulsions on ischemiareperfusion injury after unilateral lung transplantation in rats(2023) BATTOCHIO, Angela; TAVARES, Elaine; CORREIA, Aristides; ALMEIDA, Francine; CARVALHO, Priscila; GUIDO, Maria; PEGO-FERNANDES, Paulo; MARANHAO, Raul; PAZETTI, Rogerio
bookPart Transplante cardiopulmonar(2023) REIS, Flávio Pola dos; ABDALLA, Luis Gustavo; PêGO-FERNANDES, Paulo M.- Three-dimensional computed tomography reconstruction in the era of digital personalized medicine(2023) ROCHA-JUNIOR, Eserval; PEGO-FERNANDES, Paulo Manuel
- Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols(2023) GUIMARAES, Alberto Bruning; CORREIA, Aristides Tadeu; SILVA, Ronaldo Soares da; SANTOS, Elizabete Silva dos; COSTA, Natalia de Souza Xavier; DOLHNIKOFF, Marisa; MAIZATO, Marina; CESTARI, Idagene Aparecida; PEGO-FERNANDES, Paulo Manuel; CARDOSO, Paulo Francisco GuerreiroTracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold's biomechanical properties. We tested three protocols for porcine tracheal scaffold preservation immersed in PBS and alcohol 70%, in the fridge and under cryopreservation. Ninety-six porcine tracheas (12 in natura, 84 decellularized) were divided into three groups (PBS, alcohol, and cryopreservation). Twelve tracheas were analyzed after three and six months. The assessment included residual DNA, cytotoxicity, collagen contents, and mechanical properties. Decellularization increased the maximum load and stress in the longitudinal axis and decreased the maximum load in the transverse axis. The decellularization of the porcine trachea produced structurally viable scaffolds, with a preserved collagen matrix suitable for further bioengineering. Despite the cyclic washings, the scaffolds remained cytotoxic. The comparison of the storage protocols (PBS at 4 degrees C, alcohol at 4 degrees C, and slow cooling cryopreservation with cryoprotectants) showed no significant differences in the amount of collagen and in the biomechanical properties of the scaffolds. Storage in PBS solution at 4 degrees C for six months did not change the scaffold mechanics.
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