DENISE FREDIANI BARBEIRO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
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Resultados de Busca
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conferenceObject The symbiotic supplementation modulate gut microbiota, regulation beta-catenin expression and prevents weight gain in ob/ob mice with non-alcoholic fatty liver disease (NAFLD)(2020) DUARTE, Sebastiao Mauro Bezerra; STEFANO, Jose Tadeu; FRANCO, Lucas A. M.; MARTINS, Roberta Cristina Ruedas; BARBEIRO, Denise Frediani; NERI, Junia Marielle Teixeira Rodrigues; COGLIATI, Bruno; SABINO, Ester Cerdeira; CARRILHO, Flair Jose; OLIVEIRA, Claudia- Chemical Characterization and Wound Healing Property of Jacaranda decurrens Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms(2020) SERRA, Mariana B.; BARROSO, Wermerson A.; ROCHA, Claudia; FURTADO, Pablo G. R.; BORGES, Antonio C. R.; SILVA, Selma N.; TANGERINA, Marcelo M. P.; NASCIMENTO, Jessyane R. do; VILEGAS, Wagner; ALVES, Ademilton C.; BARBEIRO, Denise F.; SOUZA, Heraldo P. de; ABREU, Iracelle C.; BORGES, Marilene O. R.Background. Jacaranda decurrens Cham., known as carobinha, is prevalent in the Cerrado biome and presents popular use in treatment of dermatological diseases. The present study aimed to investigate the healing action of topical formulation of Jacaranda decurrens Cham. (FtEHJ) in mice cutaneous lesions. Methods. Phytochemical analysis of J. decurrens hydroalcoholic extract was carried out by using HPLC-PDA-ESI-MS and FIA-ESI-IT-MSn. Swiss mice were treated topically with formulation base (FtB) or Fibrinase (R) or ointment FtEHJ (15 mg/g; 50 mg/Kg). At the end of treatment periods, the inflammatory cytokines (TNF-alpha, IL-1 beta, and IL-6) in the lesions were measured by using ELISA and gene expression of TGF-beta, Collagen I, and Collagen III was demonstrated by RTqPCR method and histological evaluation. Results. Ten compounds were identified in the extract, distributed among the classes of flavonoids and triterpenes. Treatment with FtEHJ increased the wound contraction in 24 hours, such as reduction of TNF-alpha, IL-1 beta, and IL-6 (pg/mL) cytokines in the lesion. The TGF-beta and collagen gene expression was increased and the wound closure accelerated to nine days, with discrete inflammation, collagenization, and accented reepithelialization. Conclusions. The results obtained suggest chemical compounds present in the FtEHJ accelerates wound healing by being a gene expression modulator, and protein content of different molecules are involved in tissue repair.
- Muscle degradation, vitamin D and systemic inflammation in hospitalized septic patients(2020) BORGES, Rodrigo Cerqueira; BARBEIRO, Hermes Vieira; BARBEIRO, Denise Frediani; SORIANO, Francisco GarciaPurpose: To date, the relationship between systemic inflammation and muscle changes observed by ultrasonog-raphy in septic patients in clinical studies is not known. Furthermore, the role of vitamin Don muscle changes in these patients needs to be investigated. Materials and methods: Forty-five patients admitted to the ICU due to severe sepsis or septic shock. Blood samples were collected to evaluate systemic inflammation (interleukin (IL)-10, IL-1 beta, IL-1 alpha, IL-6, IL-8 and tumor necrosis factor-alpha(TNF-alpha)) and vitamin D. Muscle mass was evaluated by ultrasound during hospitalization. Clinical tests of muscle strength (Medical Research Council (MRC) scale and handgrip) were performed after the awakening of patients. Results: There was a reduction in day 2 values to hospital discharge on TNF-alpha, IL-8. IL-6 and IL-10 (p < .05). The muscle mass showed a significant decline from day 6 of the ICU. After awakening, the patients had a significant increase in muscle strength (p < .05). There was a positive association between muscle mass variation (day 2 - ICU) with absolute values of IL-8 (r = 0.38 p = .05). For muscle strength, there was a negative association between handgrip strength with IL-8 (r = -0.36 p < .05) on ICU discharge. The vitamin D showed a positive association with the handgrip strength of the day 1 of the awakening (r = 0.51 p < .05). Conclusions: In septic patients, there is an association between inflammation and changes in muscle mass and strength during ICU stay, which is similar to those observed in experimental studies. In addition, there was an association of vitamin D with recovery of muscle strength during hospitalization.
conferenceObject PERITONEAL LAVAGE IN ACUTE PANCREATITIS: DOES THE SEVERITY OF THE DISEASE INFLUENCE THE OUTCOME?(2020) SERRA, Mariana B.; BARBEIRO, Denise F.; LIMA, Thais; BARROSO, Wermerson; SORIANO, Francisco G.; MACHADO, Marcel C. C.; SOUZA, Heraldo P.