PAULO HENRIQUE BRAZ DA SILVA
Projetos de Pesquisa
Unidades Organizacionais
ODE, FO - Docente
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
- Oral hairy leukoplakia diagnosis by Epstein-Barr virus in situ hybridization in liquid-based cytology(2014) BRAZ-SILVA, P. H.; SANTOS, R. T. M.; SCHUSSEL, J. L.; GALLOTTINI, M.ObjectiveTo establish a definitive diagnosis of oral hairy leukoplakia (OHL) by insitu hybridization for Epstein-Barr virus (EBV) detection with liquid-based cytology (LBC), using the ThinPrep((R)) Pap Test, and to compare its efficacy with the traditional method of performing biopsy. MethodsThirty-three individuals divided into three groups were included in this study. Group 1 consisted of 15 human immunodeficiency virus (HIV)-positive patients with a clinical and histopathological diagnosis of OHL on the lateral border of the tongue. Group 2 consisted of 10 HIV-positive individuals with neither OHL nor other oral lesions. Group 3 consisted of 10 immunocompetent HIV-negative individuals with neither OHL nor other oral lesions. For each patient from the three groups, exfoliative LBC was performed on the lateral border of the tongue using ThinPrep. For the patients from group 1, a 6-mm-diameter punch biopsy was obtained from the same anatomic site as the brush collection to confirm the diagnosis of OHL by histopathology with insitu hybridization. Slides were prepared for morphological cellular analysis using Papanicolaou (Pap) staining, and for EBV detection using insitu hybridization. ResultsThirteen of the 15 patients from group 1 were confirmed on punch biopsy as OHL, providing the gold standard for the study. The sensitivity of LBC followed by a Pap-stained smear was 62% and the specificity was 90%. The sensitivity of LBC followed by insitu hybridization was 100% and the specificity was 100%. ConclusionsExfoliative LBC associated with EBV insitu hybridization is a simple, effective and non-invasive diagnostic tool for OHL.
- Oral and cervical HPV infection in HIV-positive and HIV-negative women attending a sexual health clinic in Sao Paulo, Brazil(2014) LIMA, Marina D. M.; BRAZ-SILVA, Paulo Henrique; PEREIRA, Sonia M.; RIERA, Catalina; COELHO, Ariane C.; GALLOTTINI, MarinaObjective: To investigate the prevalence of HPV infections in the oral and cervical mucosa of HIV-positive and HIV-negative women attending a sexual health clinic. Methods: One-hundred HIV-positive women and 100 HIV-negative women were recruited from a sexual health clinic in Sao Paulo, Brazil. All participants were given an oral and cervical examination. Cytologic samples were evaluated via HPV DNA test. Results: In oral samples, HPV DNA was observed in 11 women from the HIV-positive group and 2 from the HIV-negative group. High-risk HPV subtypes were prevalent in both groups and no difference between groups was detected. HPV DNA was detected in cervical scrapings from 41 HIV-positive women and 45 HIV-negative women (P = 0.67). No participants showed oral lesions, whereas 15 HIV-positive and 17 HIV-negative women presented with macroscopic genital lesions. Conclusion: HPV is detected more often in cervical scrapings than in oral samples. However, HPV oral shedding is more frequent in HIV-positive than in HIV-negative individuals. Concurrence of infection (high and low risk) was not observed in oral and cervical mucosa.