PAULO HENRIQUE BRAZ DA SILVA
Projetos de Pesquisa
Unidades Organizacionais
ODE, FO - Docente
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
19 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 19
- The Torque Teno Virus Titer in Saliva Reflects the Level of Circulating CD4(+) T Lymphocytes and HIV in Individuals Undergoing Antiretroviral Maintenance Therapy(2022) HONORATO, Layla; WITKIN, Steven S.; MENDES-CORREA, Maria Cassia; TOSCANO, Ana Luiza Castro Conde; LINHARES, Iara Moreno; PAULA, Anderson Vicente de; PAIAO, Heuder Gustavo Oliveira; PAULA, Vanessa Salete de; LOPES, Amanda de Oliveira; LIMA, Silvia Helena; RAYMUNDI, Vanessa de Cassia; FERREIRA, Noely Evangelista; SILVA JUNIOR, Almir Ribeiro da; ABRAHIM, Karim Yaqub; BRAZ-SILVA, Paulo Henrique; TOZETTO-MENDOZA, Tania ReginaIntroductionTorque teno virus (TTV) is a non-pathogenic virus present in body fluids. Its titer in the circulation increases in association with immune suppression, such as in HIV-infected individuals. We evaluated if the TTV titer in saliva from HIV-positive individuals undergoing antiretroviral therapy (ART) was related to the circulating CD4+ T lymphocyte concentration and the HIV titer. MethodsSaliva was collected from 276 asymptomatic individuals undergoing ART, and an additional 48 individuals positive for AIDS-associated Kaposi's Sarcoma (AIDS-KS). The salivary TTV titer was measured by gene amplification analysis. The circulating CD4+ T lymphocyte and HIV levels were obtained by chart review. ResultsTTV was detectable in saliva from 80% of the asymptomatic subjects and 87% of those with AIDS-KS. In the asymptomatic group the median log(10) TTV titer/ml was 3.3 in 200 males vs. 2.4 in 76 females (p < 0.0001). TTV titer/ml was 3.7 when HIV was acquired by intravenous drug usage, 3.2 when by sexual acquisition and 2.4 when blood transfusion acquired. The salivary TTV titer was inversely correlated with the circulating CD4+ T lymphocyte level (p < 0.0001) and positively correlated with the circulating HIV concentration (p = 0.0005). The median salivary TTV titer and circulating HIV titer were higher, and the CD4+ count was lower, in individuals positive for AIDS-KS than in the asymptomatic subjects (p < 0.0001). ConclusionThe TTV titer in saliva is a potential biomarker for monitoring immune status in individuals undergoing ART.
- Fatores de risco para osteonecrose dos maxilares relacionada à medicação e níveis salivares de IL-6 em pacientes com câncer(2022) KEMP, Aristilia Pricila Tahara; FERREIRA, Vitor Hugo Candido; MOBILE, Rafael Zancan; BRANDAO, Thais Bianca; SASSI, Laurindo Moacir; ZARPELLON, Amanda; BRAZ-SILVA, Paulo Henrique; SCHUSSEL, Juliana LucenaIntroducao: A osteonecrose dos maxilares relacionada a medicacao e uma complicacao grave da terapia antirreabsortiva e antiangiogenica, com opcao de tratamento limitada e grande impacto na qualidade de vida do paciente.Objetivo: Avaliar os fatores de risco associados a osteonecrose dos maxilares relacionada a medicacao em pacientes oncologicos em tratamento com bifosfonato Alem disso, os niveis salivares de interleucina-6 (IL-6) foram medidos para investigar sua associacao com a gravidade e o risco de osteonecrose dos maxilares relacionada a medicacao.Metodo: Estudo caso-controle com 74 pacientes com metastases osseas de tumores solidos e mieloma multiplo. Os pacientes foram divididos em tres grupos: 1) em tratamento por bifosfo-nato com osteonecrose dos maxilares relacionada a medicacao; 2) submetidos ao bifosfonato sem osteonecrose dos maxilares relacionada a medicacao; e 3) pre-tratamento de bifosfonato. Os dados demograficos e medicos dos pacientes foram coletados para avaliar o risco. A avaliacao clinica foi feita para diagnosticar osteonecrose dos maxilares relacionada a medicacao e a saliva nao estimulada foi coletada para quantificacao da IL-6.Resultados: Observou-se que os pacientes diagnosticados com osteonecrose dos maxilares rela-cionada a medicacao foram submetidos a maior numero de doses de bifosfonato (p = 0,001) e protocolo de infusao mensal (p = 0,044; OR = 7,75). Pacientes que nao tiveram acompanha-mento de rotina com dentistas especializados durante a terapia com bifosfonato e tabagismo foram associados ao osteonecrose dos maxilares relacionada a medicacao (p = 0,019; OR = 8,25 e p = 0,031; OR = 9,37, respectivamente). O grupo 1 apresentou maior frequencia de trata-mento com quimioterapia e corticosteroides concomitantes ao bifosfonato e procedimentos odontologicos cirurgicos (p = 0,129). Os niveis salivares de IL-6 nao apresentaram diferenca estatisticamente significante entre os grupos (p = 0,571) ou associacao com a gravidade do osteonecrose dos maxilares relacionada a medicacao (p = 0,923).Conclusao: Maior numero de ciclos de bifosfonato, protocolo de infusao mensal, ausen-cia de acompanhamento odontologico para manutencao da saude bucal e tabagismo foram associados ao osteonecrose dos maxilares relacionada a medicacao. O acompanhamento odon-tologico especializado durante o tratamento demonstrou ser importante na prevencao dessa complicacao. (c) 2020 Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial. Publicado por Elsevier Editora Ltda. Este e um artigo Open Access sob uma licenca CC BY (http:// creativecommons.org/licenses/by/4.0/).
- Oral lesions and SARS-CoV-2: A postmortem study(2022) ZARPELLON, Amanda; MATUCK, Bruno F.; DOLHNIKOFF, Marisa; DUARTE-NETO, Amaro N.; MAIA, Gilvan; GOMES, Sara C.; SENDYK, Daniel I.; SOUZA, Suzana C. O. M.; MAUAD, Thais; SALDIVA, Paulo H. N.; BRAZ-SILVA, Paulo H.; SILVA, Luiz F. F. da
- Is 0.5% hydrogen peroxide effective against SARS-CoV-2?(2022) ORTEGA, Karem Lopez; RECH, Bruna de Oliveira; COSTA, Andre Luiz Ferreira; SAYANS, Mario Perez; BRAZ-SILVA, Paulo Henrique
- Expression profile of SARS-CoV-2 cellular entry proteins in normal oral mucosa and oral squamous cell carcinoma(2022) SAPKOTA, Dipak; SHARMA, Sunita; SOLAND, Tine M.; BRAZ-SILVA, Paulo H.; TEH, Muy-TeckObjective: Besides angiotensin converting enzyme 2 (ACE2), an active involvement of proteases (FURIN and/or TMPRSS2) is important for cellular entry of SARS-CoV-2. Therefore, a simultaneous expression profiling of entry proteins in a tissue might provide a better risk assessment of SARS-CoV-2 infection as compared to individual proteins. In an attempt to understand the relative susceptibility of oral squamous cell carcinoma (OSCC) lesions as compared to the normal oral mucosa (NOM) for SARS-CoV-2 attachment/entry, this study examined the mRNA and protein expression profiles of ACE2, FURIN, and TMPRSS2 in the corresponding tissues using public transcriptomic and proteomics datasets. Methods and methods: Public transcriptomic and proteomics datasets (the Cancer Genome Atlas (TCGA)/the Genotype-Tissue Expression (GTEx), the Human Protein Atlas (HPA), and two independent microarray datasets) were used to examine the expression profiles of ACE2, TMPRSS2 and FURIN in NOM and OSCC. Results: ACE2, TMPRSS2, and FURIN mRNAs were detected in NOM, however, at lower levels as compared to other body tissues. Except for moderate up-regulation of FURIN, expression levels of ACE2 and TMPRSS2 mRNA were unchanged/downregulated in OSCC as compared to the NOM. Conclusions: These results indicate that NOM may serve as a possible site for SARS-CoV-2 attachment, however, to a lesser extent as compared to organs with higher expression levels of the SARS-CoV-2 entry proteins. However, the evidence is lacking to suggest that expression status of entry proteins predisposes OSCC lesions to additional risk for SARS-CoV-2 attachment/entry as compared to NOM.
- Lack of direct association between oral mucosal lesions and SARS-CoV-2 in a cohort of patients hospitalised with COVID-19(2022) SCHWAB, Gabriela; PALMIERI, Michelle; ZERBINATI, Rodrigo M.; SARMENTO, Dmitry J. S.; REIS, Thais; ORTEGA, Karem L.; KANO, Italo T.; V, Rafael A. Caixeta; HASSEUS, Bengt; SAPKOTA, Dipak; JUNGES, Roger; GIANNECCHINI, Simone; COSTA, Andre L. F.; JALES, Sumatra M. C. P.; LINDOSO, Jose A. L.; GALLO, Camila Barros; BRAZ-SILVA, Paulo H.Background COVID-19 is a disease affecting various human organs and systems, in which the virus seeks to interact with angiotensin-converting enzyme 2 receptors. These receptors are present in the oral cavity, but the direct relationship between such an interaction and possible oral manifestations of COVID-19 is still unclear. Aim The present study evaluated oral manifestations in a cohort of COVID-19 patients during the period of hospitalisation. Methods In total, 154 patients presenting moderate-to-severe forms of COVID-19 had their oral mucosa examined twice a week until the final outcome, either discharge or death. The oral alterations observed in the patients were grouped into Group 1 (pre-existing conditions and opportunistic oral lesions) and Group 2 (oral mucosal changes related to hospitalization). Results Oral lesions found in the patients of Group 1 are not suggestive of SARS-CoV-2 infection as they are mainly caused by opportunistic infections. On the other hand, oral alterations found in the patients of Group 2 were statistically (P < 0.001) related to intubation and longer period of hospitalisation. Conclusion It is unlikely that ulcerative lesions in the oral cavity are a direct manifestation of SARS-CoV-2 or a marker of COVID-19 progression.
- SARS-CoV-2 in saliva, viremia and seroprevalence for COVID-19 surveillance at a single hematopoietic stem cell transplantation center: a prospective cohort study(2022) MOBILE, Rafael Zancan; WARNAWIN, Stephanie von Stein Cubas; KOJO, Teresinha Keiko; RODRIGUES, Jessica Alline Pereira; CAVILHA, Adriana Mendes de Quadros; ZERBINATI, Rodrigo Melim; ADAMOSKI, Douglas; OLIVEIRA, Jaqueline Carvalho de; CONZENTINO, Marcelo Santos; HUERGO, Luciano Fernandes; GRADIA, Daniela Fiori; BRAZ-SILVA, Paulo Henrique; SCHUSSEL, Juliana LucenaThis prospective cohort study aims to analyze the surveillance of COVID-19 at a single hematopoietic stem cell transplantation (HSCT) center in Brazil, in 29 patients undergoing allogeneic HSCT and 57 healthcare workers (nurses and dentists), through viral shedding of SARS-CoV-2 in saliva and plasma and seroprevalence of anti-SARS-CoV-2 IgG. In addition, we report two cases with prolonged persistent detection of SARS-CoV-2 without seroconversion. The sample collection was performed seven times for patients and five times for healthcare workers. Only two patients tested positive for SARS-CoV-2 in their saliva and plasma samples (6.9%) without seroconversion. All healthcare workers were asymptomatic and none tested positive. Two patients (6.9%) and four nurses (8%) had positive serology. No dentists had positive viral detection or positive serology. Our results reflect a low prevalence of positive RT-PCR and seroprevalence of SARS-CoV-2 in patients and healthcare workers at a single HSCT center. Results have also corroborated how the rigorous protocols adopted in transplant centers were even more strengthened in this pandemic scenario.
- Use of saliva and RT-PCR screening for SARS-CoV-2 variants of concern: Surveillance and monitoring(2022) ZERBINATI, Rodrigo Melim; PALMIERI, Michelle; SCHWAB, Gabriela; FELIX, Alvina Clara; MARTINHO, Herculano; GIANNECCHINI, Simone; TO, Kelvin Kai-Wang; LINDOSO, Jose Angelo Lauletta; ROMANO, Camila Malta; BRAZ-SILVA, Paulo HenriqueGenomic surveillance has been applied since the beginning of the COVID-19 pandemic to track the spread of the virus, leading to the characterization of multiple SARS-CoV-2 variants, including variants of concern (VOC). Although sequencing is the standard method, a rapid molecular test for screening and surveillance of VOC is considered for detection. Furthermore, using alternative saliva as specimen collection facilitates the implementation of a less invasive, self-collected sample. In this study, we applied a combinatory strategy of saliva collection and reverse transcription polymerase chain reaction (RT-PCR) for SARS-CoV-2 VOC detection. Saliva samples from patients attending a tertiary hospital with suspected COVID-19 were collected and SARS-CoV-2 RNA was detected using SARS-CoV-2 RT-qPCR reagent kit (PerkinElmer). Positive saliva samples were screened for SARS-CoV-2 VOC with previously described RT-PCR for Alpha, Beta, and Gamma variants. Saliva samples were positive in 171 (53%) of 324 tested. A total of 108 (74%) from positive samples were also positive for VOC by RT-PCR screening. Those samples were found between January and August 2021. This approach allowed us to successfully use an alternative and complementary tool to genomic surveillance to monitor the circulation of SARS-CoV-2 VOC in the studied population.
- MALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-19(2022) LAZARI, Lucas C.; ZERBINATI, Rodrigo M.; ROSA-FERNANDES, Livia; SANTIAGO, Veronica Feijoli; ROSA, Klaise F.; ANGELI, Claudia B.; SCHWAB, Gabriela; PALMIERI, Michelle; SARMENTO, Dmity J. S.; MARINHO, Claudio R. F.; ALMEIDA, Janete Dias; TO, Kelvin; GIANNECCHINI, Simone; WRENGER, Carsten; SABINO, Ester C.; MARTINHO, Herculano; LINDOSO, Jose A. L.; DURIGON, Edison L.; BRAZ-SILVA, Paulo H.; PALMISANO, GiuseppeBackground The SARS-CoV-2 infections are still imposing a great public health challenge despite the recent developments in vaccines and therapy. Searching for diagnostic and prognostic methods that are fast, low-cost and accurate are essential for disease control and patient recovery. The MALDI-TOF mass spectrometry technique is rapid, low cost and accurate when compared to other MS methods, thus its use is already reported in the literature for various applications, including microorganism identification, diagnosis and prognosis of diseases. Methods Here we developed a prognostic method for COVID-19 using the proteomic profile of saliva samples submitted to MALDI-TOF and machine learning algorithms to train models for COVID-19 severity assessment. Results We achieved an accuracy of 88.5%, specificity of 85% and sensitivity of 91.5% for classification between mild/moderate and severe conditions. When we tested the model performance in an independent dataset, we achieved an accuracy, sensitivity and specificity of 67.18, 52.17 and 75.60% respectively. Conclusion Saliva is already reported to have high inter-sample variation; however, our results demonstrates that this approach has the potential to be a prognostic method for COVID-19. Additionally, the technology used is already available in several clinics, facilitating the implementation of the method. Further investigation using a larger dataset is necessary to consolidate the technique.
- Primary dental care treatment in primary Sjogren's syndrome: a possible role in improving salivary flow rate(2022) MARTINS, V. A. O.; FLORIANO, T. F.; LEON, E. P.; VILLAMARIN, L. E. B.; DEVEZA, G. B. H.; AIKAWA, N. E.; SILVA, C. A. A.; KUPA, L. V. K.; PERES, M. P. S. M.; BRAZ-SILVA, P. H.; BONFA, E.; PASOTO, S. G.Objective Primary Sjogren's syndrome (pSS) is an inflammatory chronic disorder that mainly affects exocrine glands. Additionally, oral infections can aggravate the glandular dysfunction. However, data on primary dental care (PDC) treatment in pSS are scarce. This study aimed to appraise the impact of PDC on the Xerostomia Inventory (XI), unstimulated/stimulated salivary flow rates and salivary cytokine profile in pSS. Methods Fifty-two pSS patients and 52 sex- and age-matched control participants without systemic autoimmune diseases were included in a prospective study. At inclusion, all participants were assessed through a standardised protocol, measurement of salivary pro-inflammatory cytokines, and underwent PDC. Dental procedures included: oral hygiene guidance, restorative treatment of caries, surgical removal of residual roots and impacted or partially erupted teeth, cysts, supra and subgingival periodontal scaling and treatment of soft tissue disorders (removal of lesions and treatment of opportunistic infections). After 3 months, the clinical/laboratorial assessments were repeated. Results At inclusion, the Decayed, Missing and Filled Teeth (DMFT) index was higher in the pSS patients than in the control group (13.3 +/- 8.2 vs. 8.6 +/- 6.2, p=0.002), whereas periodontal parameters were comparable in both groups (p>0.05). After PDC, 26.9% of pSS patients showed a reduction of at least 6 points (clinical improvement) in XI, but mean XI remained unchanged (p=0.285). PDC resulted in an increase in mean unstimulated (p<0.001) and stimulated (p=0.001) salivary flow rates in pSS, with no change in salivary cytokine profile (p=0.05). Conclusion PDC promoted improvement in unstimulated and stimulated salivary flow rates in pSS. This novel finding reinforces the recommendation of this strategy for pSS patients. Clinicaltrials.gov (Identifier: NCT03711214).