JOSE ANGELO LAULETTA LINDOSO
Projetos de Pesquisa
Unidades Organizacionais
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
8 resultados
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bookPart 0 Citação(ões) na Scopus Parasitological, serological diagnosis of caninevisceral leishmaniasis, association of neutrophilic inflammatory infiltrate and transmission potential: A review(2013) COSTA, F. A. L.; CARVALHO, A. A.; LINDOSO, J. A. L.; GOTO, H.Canine visceral leishmaniasis (CVL) is important in the transmission cycle of Leishmania (Leishmania) infantum as it constitutes the main reservoir of the parasite in the peridomestic cycle. We revise the laboratory diagnosis of CVL since control program in some parts of the world including Brazil has in its guideline the culling of infected dogs based on anti-Leishmania antibody detection. But the serological techniques have limitation regarding the specificity and sensitivity. The confirmation of infection is performed by parasitological analysis that is considered the gold standard since it presents 100% specificity. Whereas sensitivity, specificity and predictive values of the parasitological methods are critical aspects for the diagnosis of CVL per se and for the dogs as source of parasite for transmission, parameters that may give indication of this potential are important to unveil. Here we discuss the serological diagnosis and then the parasitological diagnosis with focus on neutrophilic infiltrate in the tissue related to transmission potential. © 2013 Nova Science Publishers, Inc. All rights reserved.conferenceObject Evaluation of immunochromatographic assay with recombinant antigen K39, using whole blood, serum and oral fluid, in the diagnosis of visceral leishmaniasis in Brazilian endemic areas(2015) CELESTE, B. J.; SANCHEZ, M. C. A.; ALMEIDA, R. P. D.; BRANCO, C. M. F. Castelo; LINDOSO, J. A. L.; PANIAGO, A. M. M.; QUEIROZ, I. T.; FUJIMORI, M.; CARAMELO, L. D. C.; GOTO, H.- Para-kala-azar dermal leishmaniasis in a patient in Brazil: a case report (vol 51, pg 106, 2018)(2018) LINDOSO, J. A. L.; MOREIRA, C. H. V.; CELESTE, B. J.; OYAFUSO, L. K. M.; FOLEGATTI, P. M.; ZIJLSTRA, E. E.
- Dynamics of immunosuppression in hamsters with experimental visceral leishmaniasis(2011) FAZZANI, C.; GUEDES, P. A.; SENA, A.; SOUZA, E. B.; GOTO, H.; LINDOSO, J. A. L.Immunosuppression has been reported to occur during active visceral leishmaniasis and some factors such as the cytokine profile may be involved in this process. In the mouse model of cutaneous leishmaniasis using Leishmania (Leishmania) major, the Th1 response is related to protection while the Th2 response is related to disease progression. However, in hamsters, which are considered to be an excellent model for the study of visceral leishmaniasis, this dichotomy is not observed. Using outbred 45- to 60-day-old (140 to 150 g) male hamsters infected intraperitoneally with 2 x 10(7) L. (L.) chagasi amastigotes, we evaluated the immune response of spleen cells and the production of cytokines. We used 3 to 7 hamsters per group evaluated. We detected a preserved response to concanavalin A measured by index of proliferation during all periods of infection studied, while a proliferative response to Leishmania antigen was detected only at 48 and 72 h post-infection. Messenger RNA from cytokines type 1 (IL-2, TNF-alpha, IFN-gamma) and type 2 (IL-4, IL-10 and TGF-beta) detected by reverse transcriptase polymerase chain reaction and produced by spleen cells showed no qualitative difference between control non-infected hamsters and infected hamsters during any period of infection evaluated. Cytokines were measured by the DNA band intensity on agarose gel using the Image Lab 1D L340 software with no differences observed. In conclusion, the present results showed an antigen-dependent immunosuppression in hamsters with active visceral leishmaniasis that was not related to the cytokine profile.
conferenceObject Diffent genotypes of Leishmania infantum occurring in visceral leishmaniasis in HIV and not-HIV-infected patients from the new world(2015) SILVA, I. T. B. Queiroz e; ALONSO, D. P.; FORTALEZA, C. M. C. B.; RIBOLLA, P. E. M.; LINDOSO, J. A. L.- Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study(2020) CUNHA, M. A.; CELESTE, B. J.; KESPER, N.; FUGIMORI, M.; LAGO, M. M.; IBANES, A. S.; OUKI, L. M.; SIMOES NETO, E. A.; FONSECA, F. F.; SILVA, M. A. L.; BARBOSA JUNIOR, W. L.; LINDOSO, J. A. L.BackgroundThere is little information about the frequency of Leishmania infection in asymptomatic people living with HIV (PLWH) and about the performance of laboratory diagnostic methods in coinfected patients in Latin America. The main objective of this study is to evaluate the frequency of Leishmania spp. infection in HIV-infected patients living in an urban area in Brazil.MethodsTo detect Leishmania infection, diagnostic tests were performed to detect anti-Leishmania antibodies (ELISA using Leptomonas seymouri antigens; ELISA using rK39 antigens; ELISA using rK28 antigens; indirect fluorescent-antibody test (IFAT); direct agglutination test (DAT)) and Leishmania DNA (polymerase chain reaction (PCR) with the target genes kDNA and ITS-1).ResultsThe frequency of at least one positive test was 15%. For ELISA using Leptomonas antigens and IFAT, there was an association between CD4+ T lymphocyte counts and test positivity, with a higher positivity of these tests in more immunosuppressed patients (CD4+ T cell count <200/mm(3)).ConclusionsAccording to our data, there was a high prevalence of Leishmania spp. infections in this population living with HIV. Although there is the possibility of cross-reaction, some tests that are considered highly specific for the diagnosis of Leishmania infection were positive. There was also an association between the positivity of some tests studied and lower values of CD4+ T lymphocytes.
conferenceObject Specie-specific diferenciation of Leishmania through real time PCR and high resolution melt(2015) SILVA, R. E. C.; FARIAS, L.; DIAS, D. B.; SILVA, E. O. Mello; SOARES, S. R. C.; LINDOSO, J. A. L.- Recombinant protein KR95 as an alternative for serological diagnosis of human visceral leishmaniasis in the Americas (vol 18, e0282483, 2023)(2023) FUJIMORI, M.; VALENCIA-PORTILLO, R. T.; LINDOSO, J. A. L.; CELESTE, B. J.; ALMEIDA, R. P. de; COSTA, C. H. N.