JOSE ANGELO LAULETTA LINDOSO
Projetos de Pesquisa
Unidades Organizacionais
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- First case report of monkeypox in Brazil: clinical manifestations and differential diagnosis with sexually transmitted infections(2022) LIMA, Evelyn Lepka de; BARRA, Luiz Alberto Costa; BORGES, Luciana Marques Sansao; MEDEIROS, Lucas Alberto; TOMISHIGE, Marcia Y. S.; SANTOS, Lucas de Souza Loureiro Abbud; SILVA, Anderson Jose Dias da; RODRIGUES, Camila Cristina Martini; AZEVEDO, Luiz Cesar Fernandes de; VILLAS-BOAS, Lucy Santos; SILVA, Camila Alves Maia da; COLETTI, Thais Moura; MANULI, Erika R.; CLARO, Ingra Morales; ROMANO, Camila Malta; RAMUNDO, Mariana Severo; MOUTINHO, Tomas; SABINO, Ester Cerdeira; LINDOSO, Jose Angelo Lauletta; FIGUEIREDO-MELLO, ClaudiaIn 2022, an outbreak of monkeypox is being reported in non-endemic areas, with unusual clinical manifestations. The detailed clinical description of the first patient that received the diagnosis of monkeypox in Brazil is reported here, whose clinical manifestations can easily lead to misdiagnosis of sexually transmitted infections. A 41 years old male presented to an emergency room with a vesicular rash with eight days of evolution. He had traveled to Portugal and Spain and reported non-penetrative sexual involvement with three different male individuals. On the third day of symptoms, he sought medical care and received empirical treatment directed to sexually transmitted infections. As the symptoms did not improve, he sought medical attention at an infectious disease referral center presenting, on admission, an ulcerated penile lesion with central necrotic crusts, a disseminated pleomorphic skin rash and an oropharyngeal ulcer. The monkeypox diagnosis was suspected due to the characteristics of the lesions and the history of intimate contact with casual partners, and it was later confirmed by sequencing the almost complete monkeypox genome. The patient was hospitalized for pain control, which required opiate administration. He developed a secondary bacterial infection on the penile lesions, which were treated with oral antibiotics. He was discharged after 14 days, with lesions in process of re-epithelialization. Given the current outbreak, we must consider the possibility of monkeypox in patients with suggestive lesions, anywhere on the body (including the genitals), added to an epidemiological link or history of intimate contact with strangers or casual partners.
conferenceObject CHAGAS DISEASE: A PROSPECTIVE THERAPEUTIC COHORT WITH 12 MONTHS FOLLOW-UP, ANALYZING ADVERSE DRUG REACTIONS, THERAPEUTIC FAILURE AND SEEKING FOR BIOMARKERS""(2017) MOREIRA, Carlos H.; CARVALHO, Noemia B.; FERRUFINO, Rosario Q.; OLIVEIRA, Lea C.; LINDOSO, Jose A.; MANULI, Erika; SOUZA, Marcela De; SABINO, Ester C.- MALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-19(2022) LAZARI, Lucas C.; ZERBINATI, Rodrigo M.; ROSA-FERNANDES, Livia; SANTIAGO, Veronica Feijoli; ROSA, Klaise F.; ANGELI, Claudia B.; SCHWAB, Gabriela; PALMIERI, Michelle; SARMENTO, Dmity J. S.; MARINHO, Claudio R. F.; ALMEIDA, Janete Dias; TO, Kelvin; GIANNECCHINI, Simone; WRENGER, Carsten; SABINO, Ester C.; MARTINHO, Herculano; LINDOSO, Jose A. L.; DURIGON, Edison L.; BRAZ-SILVA, Paulo H.; PALMISANO, GiuseppeBackground The SARS-CoV-2 infections are still imposing a great public health challenge despite the recent developments in vaccines and therapy. Searching for diagnostic and prognostic methods that are fast, low-cost and accurate are essential for disease control and patient recovery. The MALDI-TOF mass spectrometry technique is rapid, low cost and accurate when compared to other MS methods, thus its use is already reported in the literature for various applications, including microorganism identification, diagnosis and prognosis of diseases. Methods Here we developed a prognostic method for COVID-19 using the proteomic profile of saliva samples submitted to MALDI-TOF and machine learning algorithms to train models for COVID-19 severity assessment. Results We achieved an accuracy of 88.5%, specificity of 85% and sensitivity of 91.5% for classification between mild/moderate and severe conditions. When we tested the model performance in an independent dataset, we achieved an accuracy, sensitivity and specificity of 67.18, 52.17 and 75.60% respectively. Conclusion Saliva is already reported to have high inter-sample variation; however, our results demonstrates that this approach has the potential to be a prognostic method for COVID-19. Additionally, the technology used is already available in several clinics, facilitating the implementation of the method. Further investigation using a larger dataset is necessary to consolidate the technique.
conferenceObject BENZNIDAZOLE-RELATED ADVERSE DRUG REACTIONS IN BRAZILIAN PATIENTS WITH CHAGAS DISEASE(2015) MOREIRA, Carlos H.; CARVALHO, Noemia B.; FERRUFINO, Rosario Q.; GUASTINI, Cristina M.; LINDOSO, Jose Angelo L.; OLIVEIRA, Lea C.; MANULI, Erika R.; SOUZA, Marcela; SABINO, Ester C.- Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022(2022) CLARO, Ingra Morales; ROMANO, Camila Malta; CANDIDO, Darlan da Silva; LIMA, Evelyn Lepka de; LINDOSO, Jose Angelo Lauletta; RAMUNDO, Mariana Severo; MOREIRA, Filipe Romero Rebello; BARRA, Luiz Alberto Costa; BORGES, Luciana Marques Sansao; MEDEIROS, Lucas Alberto; TOMISHIGE, Marcia Y. S.; MOUTINHO, Tomas; SILVA, Anderson Jose Dias da; RODRIGUES, Camila Cristina Martini; AZEVEDO, Luiz Cesar Fernandes de; VILLAS-BOAS, Lucy Santos; SILVA, Camila Alves Maia da; COLETTI, Thais Moura; MANULI, Erika R.; O'TOOLE, Aine; QUICK, Joshua; LOMAN, Nicholas; RAMBAUT, Andrew; FARIA, Nuno R.; FIGUEIREDO-MELLO, Claudia; SABINO, Ester CerdeiraMonkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.