JORGE HALLAK
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
60 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 60
conferenceObject ELEVATED ROS LEVELS AND DNA FRAGMENTATION IN SPERM FROM CONVALESCENT MEN IN SARS-COV-2 INFECTION(2022) HALLAK, Jorge; BERNARDES, Felipe; TEIXEIRA, Thiago Afonso Carvalho Celestino; SALDIVA, Paulo Hilario Nascimento; KALLAS, Esper Georges; CALDINI, Elia Tamaso Espin Garcia; DUARTE NETO, Amaro Nunes; FAQUINETI, Heloisa; JESUS, Vinicius Luiz Menezes; GUTIERREZ, Raul Segundo Sanchez; DREVET, Joel R.- An easy, reproducible and cost-effective method for andrologists to improve the laboratory diagnosis of non-obstructive azoospermia: a novel microcentrifugation technique(2016) MONTEIRO, Rosa Alice Casemiro; PARIZ, Juliana Risso; PIERI, Patricia de Campos; HALLAK, JorgeThis study describes a new method of microcentrifugation as an improved, viable, cost-effective option to the classical Cytospin apparatus to confirm azoospermia. Azoospermic semen samples were evaluated for cryptozoospermia by a centrifugation method similar to that of World Health Organization guidelines (2010; entire specimen centrifuged at 3000g for 15 min, and aliquots of the pellet examined). Then, if no sperm were detected, the pellet from that procedure was resuspended in culture medium, centrifuged (2000g for 15 min), and the entire pellet spread on a 4 X 6mm area of a slide and stained using the Christmas tree method (Nuclear-Fast solution and picric acid). The entire stained area was examined for the presence or absence of sperm. A total of 148 azoospermic samples (after standard WHO diagnosis) were included in the study and 21 samples (14.2%) were identified as sperm-positive. In all microcentrifugation slides, intact spermatozoa could be easily visualized against a clear background, with no cellular debris. This novel microcentrifugation technique is clearly a simple and effective method, with lower cost, increasing both sensitivity and specificity in confirming the absence or presence of spermatozoa in the ejaculate. It may represent a step forward of prognostic value to be introduced by andrology laboratories in the routine evaluation of patients with azoospermia in the initial semen analysis.
- Baggio-Yoshinari syndrome as a sexually transmitted infection: a possibly way of transmission?(2022) OLIVEIRA, Yasmin C.; HSIEH, Mariana K.; TEIXEIRA, Thiago A.; CARRASCO, Caio H.; HALLAK, Jorge
- Male Sexual Quality Of Life Is Maintained Satisfactorily Throughout Life In The Amazon Rainforest(2018) TEIXEIRA, Thiago; NAZIMA, Maira; HALLAK, JorgeIntroduction: The Amazon Rainforest is a cradle of biodiversity, where different ethnic groups have specific sexual habits. Aims: To define the average sexual quality of life of Amazonian men 18 to 69 years old, evaluate the influence of aging on their sexual function, and calculate the prevalence of premature ejaculation, delayed ejaculation, and hypoactive sexual desire disorder. Methods: A cross-sectional quantitative probability sample study was performed with a demographically representative population (N = 385), with data collected privately at participants' houses, including men who had been sexually active for a minimum of 6 months. The Male Sexual Quotient (MSQ) was used to measure sexual satisfaction and function. Statistical analysis was performed with SPSS 21.0 using the Kruskal-Wallis test (P <.05), and a multiple linear regression analysis was performed to investigate which factors could predict participants' quality of sexual life. Main Outcome Measures: MSQ scores. Results: The response rate was 81.69%. The mean age was 36.00 +/- 12.95 years, and most men had mixed ethnicity (63.11%), were self-employed (42.07%), had a monthly earned income of US$0 to US$ 460 (46.75%), and were single (36.10%). The mean MSQ score was 80.39 +/- 12.14 (highly satisfied). None of the demographic characteristics showed a statistically significant influence on sexual satisfaction. The difference in quality of sexual life was statistically significant compared with age (P <.01). The domains of desire (P <.01), partner satisfaction (P = .04), and erection quality (P <.01, P = .03, P = .02) were statistically significant. Prevalences of sexual dysfunctions were 36.54% for premature ejaculation, 6.5% for delayed ejaculation, and 11.69% for hypoactive sexual desire disorder. Conclusions: Independent of age, these men have an excellent quality of sexual life. Sexual domains such as desire, partner satisfaction, and erection quality are related to the correlation between sexual quality of life and aging. However, the prevalence of premature ejaculation seems to be slightly higher than in other parts of the world.
conferenceObject SIGNIFICANT IMPROVEMENT IN SPERM MOTILITY IN SEMEN SAMPLES FROM ASTHENOZOOSPERMIC MEN BY USING CONTROLLED INCUBATION PROTOCOLS WITH SINGLE CONTINUOUS CULTURE MEDIUM (CSCM (R)).(2020) PARIZ, Juliana Risso; RANEA, Caroline; HALLAK, Jorge- Repeated inhalation of crack-cocaine affects spermatogenesis in young and adult mice(2012) PIRES, A.; PIERI, P.; HAGE, M.; SANTOS, A. B. G.; MEDEIROS, M. C. R.; GARCIA, R. C. T.; YONAMINE, M.; HALLAK, J.; SALDIVA, P. H. N.; ZORZETTO, J. C.; BUENO, H. M. S.To investigate the effects of repeated crack-cocaine inhalation on spermatogenesis of pubertal and mature Balb/c mice, ten young (Y-ex) and ten adult (A(ex)) Balb/c mice were exposed to the smoke from 5 g of crack with 57.7% of pure cocaine in an inhalation chamber, 5 days/week for 2 months. The young (Y-c) and adult (A(c)) control animals (n = 10) were kept in a specially built and controlled animal house facility. The morphologic analysis of both testes of all animals included the analysis of quantitative and qualitative histologic parameters to assess the effect of crack-cocaine on spermatogenesis and Leydig cells. Apoptosis was determined by immunolabeling with caspase-3 antibodies. Compared to the Y-c animals, Y-ex animals showed a significant reduction in the number of stage VII tubules per testis (p = 0.02), Sertoli cells (p < 0.001) and elongated spermatids (p = 0.001). Comparisons between the Y-ex and A(ex) groups identified a significant reduction in the number of Sertoli cells (p < 0.001) and round spermatids (p < 0.001) in the Y-ex group and a significant increase in apoptotic Leydig cells (p = 0.04) in the A(ex) group. The experimental results indicate that crack-cocaine smoke inhalation induced spermatogenesis disruption in chronically exposed mice, particularly in pubertal mice.
conferenceObject INTRACYTOPLASMIC MORPHOLOGICALLY SELECTED SPERM INJECTION OUTCOMES: THE ROLE OF SPERM PREPARATION TECHNIQUES(2013) HALLAK, Jorge; SETTI, Amanda; FIGUEIRA, Rita de Cassia; VINGRIS, Livia; IACONELLI JR., Assumpto; BORGES JR., Edson- Oxidative Stress & Male Infertility - A necessary and conflicted indissociable marriage: How and when to call for evaluation?(2021) HALLAK, Jorge; TEIXEIRA, Thiago A.
conferenceObject CORRELATION BETWEEN LIBIDO AND TESTOSTERONE: ESTRADIOL RATIO IN MEN WITH SEXUAL DYSFUNCTION(2023) NETO, R. Pedrenho; NASCIMENTO, B. Chiesa Gouveia; NUNES, R. Dos Santos Sobreira; GISMONDI, J. Pedro; BESSA JR., J. De; NAHAS, W. Carlos; HALLAK, J.