SERGIO PAULO BYDLOWSKI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
5 resultados
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bookPart Farmacogenética nas doenças hematológicas(2016) BYDLOWSKI, Sérgio Paulo; LEVY, Debora- Computer analysis of genetic parentage: application in equine diversity maintenance in Brazil.(2016) ATOMIYAMA, A.; LAURETTO, M. S.; NAKANO, F.; STERN, J. M.; LEVY, D.; BYDLOWSKI, S. P.; BYDLOWSKI, C. R.
- Decreased circulating thrombomodulin is improved by tadalafil therapy in hypoxemic patients with advanced pulmonary arterial hypertension(2016) MAEDA, N. Y.; CLAVE, M. M.; BYDLOWSKI, S. P.; LOPES, A. A.Introduction: Advanced pulmonary arterial hypertension (PAH) in patients with congenital cardiac communications and right -to -left shunting (Eisenmenger syndrome - PAH-ES) is associated with hypoxemia and decreased circulating levels o{thrombomodulin (TM), probably reflecting decreased endothelial TM production. The combination of these two factors has been shown to induce fibrin deposition, with increased risk of thrombosis, a well known complication in this syndrome. Patients and methods: We tested the hypothesis that vasodilator therapy with the phosphocliesterase-5 inhibitor taclalafil, an approved drug for management of PAH could improve endothelial dysfunction markers, in particular plasma TM, in addition to improving the physical capacity (expected effect of pulmonary vasodilatation) in PAHES patients. This was a prospective observational study of treatment naive patients subjected to specific PAH therapy. Fifteen patients aged 12 to 51 years (median 30 years) were treated for 6 months with a single daily dose of 40 mg oral tadalafil. The physical capacity (distance walked during the 6 -min walk test - 6MWD), systemic oxygen saturation and laboratory parameters were measured at baseline, and 90 days and 180 days of treatment. Results: Plasma TM, which was decreased at baseline compared to controls (p < 0.001) increased at 90 and 180 days (p = 0.003), and this was directly related (r = 0.57, p = 0.026) to improvement of oxygen saturation (p = 0.008). Heightened baseline tissue -type plasminogen activator decreased during treatment (p = 0.010), while heightened von Willebrancl factor antigen remained unchanged. The 6MWD improved significantly (p < 0.001). Conclusion: Tadalafil therapy improved circulating TM and tissue -type plasminogen activator, in addition to improving the physical capacity and oxygen saturation in PAM ES patients. (C) 2016 Published by Elsevier Ltd.
- Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score(2016) TEKIN, Nilgun; OMIDVAR, Nader; MORRIS, Tim Peter; CONGET, Paulette; BRUNA, Flavia; TIMAR, Botond; GAGYI, Eva; BASAK, Ranjan; NAIK, Omkar; AUEWARKUL, Chirayu; SRITANA, Narongrit; LEVY, Debora; CERCI, Juliano Julio; BYDLOWSKI, Sergio Paulo; PEREIRA, Juliana; DIMAMAY, Mark Pierre; NATIVIDAD, Filipinas; CHUNG, June-Key; BELDER, Nevin; KUZU, Isinsu; PAEZ, Diana; DONDI, Maurizio; CARR, Robert; OZDAG, Hilal; PADUA, Rose AnnAs a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to validate the previously reported 6-gene (LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2) model as predictor of prognosis in DLBCL. To avoid variation, all samples were tested in a single centre and single platform. This study comprised 8 countries (Brazil, Chile, Hungary, India, Philippines, S. Korea, Thailand and Turkey). Using the Kaplan-Meier and log rank test on patients (n=162) and two mortality risk groups (with those above and below the mean representing high and low risk groups) confirmed that the 6-gene predictor score correlates significantly with overall survival (OS, p< 0.01) but not with event free survival (EFS, p= 0.18). Adding the International Prognostic Index (IPI) shows that the 6-gene predictor score correlates significantly with high IPI scores for OS (p< 0.05), whereas those with low IPI scores show a trend not reaching significance (p= 0.08). This study defined an effective and economical qRT-PCR strategy and validated the 6-gene score as a predictor of OS in an international setting.
conferenceObject DIFFUSE LARGE B-CELL LYMPHOMA (DLBCL) STUDY REVEALS BIOLOGICAL HETEROGENEITY BETWEEN ETHNICALLY DIVERSE COUNTRIES; VALIDATION OF 6-GENE PROGNOSTIC SCORE IN AN INTERNATIONAL COHORT(2016) TEKIN, N.; OMIDVAR, N.; MORRIS, T.; TIMAR, B.; GAGYI, E.; CONGET, P.; BRUNA, F.; BASAK, R.; NAIK, O.; AUEWARAKUL, C. Udomsakdi; SRITANA, N.; LEVY, D.; BYDLOWSKI, S. P.; PEREIRA, J.; DIMAMAY, M. P.; NATIVIDAD, F.; CHUNG, J. K.; BELDER, N.; KUZU, I.; PAEZ, D.; DONDI, M.; CARR, R.; OZDAG, H.; PADUA, R. A.