FABIO FERNANDES MORATO CASTRO
Projetos de Pesquisa
Unidades Organizacionais
FM, Faculdade de Medicina
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina - Líder
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina - Líder
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
- Proteomic characterization of the multiple forms of the PLAs from the venom of the social wasp Polybia paulista(2011) SANTOS, Lucilene Delazari dos; MENEGASSO, Anally Ribeiro da Silva; PINTO, Jose Roberto Aparecido dos Santos; SANTOS, Keity Souza; CASTRO, Fabio Morato; KALIL, Jorge Elias; PALMA, Mario SergioThe phospholipases A(1) (PLA(1)s) from the venom of the social wasp Polybia paulista occur as a mixture of different molecular forms. To characterize the molecular origin of these structural differences, an experimental strategy was planned combining the isolation of the pool of PLAs from the wasp venom with proteomic approaches by using 2-D, MALDI-TOF-TOF MS and classical protocols of protein chemistry, which included N- and C-terminal sequencing. The existence of an intact form of PLA(1) and seven truncated forms was identified, apparently originating from controlled proteolysis of the intact protein; in addition to this, four of these truncated forms also presented carbohydrates attached to their molecules. Some of these forms are immunoreactive to specific-IgE, while others are not. These observations permit to raise the hypothesis that naturally occurring proteolysis of PLA(1), combined with protein glycosylation may create a series of different molecular forms of these proteins, with different levels of allergenicity. Two forms of PLA(2)s, apparently related to each other, were also identified; however, it was not possible to determine the molecular origin of the differences between both forms, except that one of them was glycosylated. None of these forms were immunoreactive to human specific IgE.
- Allergic reactions to manioc (Manihot esculenta Crantz): Identification of novel allergens with potential involvement in latex-fruit syndrome(2011) SANTOS, Keity Souza; GALVAO, Clovis Eduardo; GADERMAIER, Gabriele; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; MISUMI, Denise Shimbo; YANG, Ariana Campos; FERREIRA, Fatima; PALMA, Mario Sergio; KALIL, Jorge; CASTRO, Fabio Fernandes Morato