FABIO FERNANDES MORATO CASTRO
Projetos de Pesquisa
Unidades Organizacionais
FM, Faculdade de Medicina
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina - Líder
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina - Líder
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- The international survey on the management of allergic rhinitis by physicians and patients (ISMAR)(2015) BAENA-CAGNANI, Carlos E.; CANONICA, Giorgio W.; HELAL, Mohamed Zaky; GOMEZ, Rene Maximiliano; COMPALATI, Enrico; ZERNOTTI, Mario E.; SANCHEZ-BORGES, Mario; CASTRO, Fabio F. Morato; AGUTTES, Margarita Murrieta; LOPEZ-GARCIA, Aida; TADROS, Faheem A.Epidemiologic studies suggest that the prevalence of allergic rhinitis (AR) is rising worldwide. Several reports, in fact, indicate increasing trends in the prevalence of AR especially in developing countries, likely related to the environment and climate changes and the adoption of an urbanized Western lifestyle. The primary objective of the present study was to collect information about management in real-life settings, including a characterization of typical patients' profile referring to physicians, the disease features, the common approaches to diagnostic assessments and therapeutic decisions. This was an international, multicenter, cross-sectional study conducted in adults or children (>= 6 years) suffering from rhinitis confirmed by physician's diagnosis for at least one year. The 234 physicians who participated in the study included a total of 2778 patients in Egypt, Mexico, Brazil, Colombia, Guatemala, Iran, Venezuela, Argentina, Israel, Kuwait and United Arab Emirates. It was found that clinical history was the selected tool to diagnose and categorize AR patients (97.1%), with less than half of patients undergoing allergy testing, may be explaining the scarce use of immunotherapy on management of disease. Out of 2776 patients, 93.4% had somehow received a recommendation to avoid allergens and irritant agent exposure. Notably, 91.4% were receiving at least one treatment at the time of the survey, mostly oral antihistamines (79.7%) and intranasal corticosteroids (66.3%). Oral antihistamines, intranasal steroids and decongestants were considered both safe and effective by patients and physicians, preferring oral and nasal route of administration. The ISMAR registry was designed according to the most accepted epidemiological recommendations, and provides interesting information regarding the management of rhinitis from a patient and physician points of view, with many similarities between the participating countries. Further efforts are required to better manage AR and its comorbidities.
- IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy(2023) FIGO, Daniele Danella; MACEDO, Priscilla Rios Cordeiro; GADERMAIER, Gabriele; REMUZGO, Cesar; CASTRO, Fabio Fernandes Morato; KALIL, Jorge; GALVAO, Clovis Eduardo Santos; SANTOS, Keity SouzaSublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific immunotherapy with peptide vaccines is used far less, but it is of great interest in the treatment of allergic reactions, as it precludes the drawbacks of allergen extracts. The ideal peptide candidates would bind to IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences of main allergens, Der p 1, 2, 5, 7, 10, 23 and Blo t 5, 6, 12, 13, were included in a 15-mer peptide microarray and tested against pooled sera from 10 patients pre- and post-1-year SLIT. All allergens were recognized to some extent by at least one antibody isotype and peptide diversity was higher post-1-year SLIT for both antibodies. IgE recognition diversity varied among allergens and timepoints without a clear tendency. Der p 10, a minor allergen in temperate regions, was the molecule with more IgE-peptides and might be a major allergen in populations highly exposed to helminths and cockroaches, such as Brazil. SLIT-induced IgG4 epitopes were directed against several, but not all, IgE-binding regions. We selected a set of peptides that recognized only IgG4 or were able to induce increased ratios of IgG4:IgE after one year of treatment and might be potential targets for vaccines.
- Guidance to 2018 good practice: ARIA digitally-enabled, integrated, person-centred care for rhinitis and asthma(2019) BOUSQUET, J.; BEDBROOK, A.; CZARLEWSKI, W.; ONORATO, G. L.; ARNAVIELHE, S.; LAUNE, D.; MATHIEU-DUPAS, E.; FONSECA, J.; COSTA, E.; LOURENCO, O.; MORAIS-ALMEIDA, M.; TODO-BOM, A.; ILLARIO, M.; MENDITTO, E.; CANONICA, G. W.; CECCHI, L.; MONTI, R.; NAPOLI, L.; VENTURA, M. T.; FEO, G. De; FOKKENS, W. J.; CHAVANNES, N. H.; REITSMA, S.; CRUZ, A. A.; SILVA, J. da; SERPA, F. S.; LARENAS-LINNEMANN, D.; PEREZ, J. M. Fuentes; HUERTA-VILLALOBOS, Y. R.; RIVERO-YEVERINO, D.; RODRIGUEZ-ZAGAL, E.; VALIULIS, A.; DUBAKIENE, R.; EMUZYTE, R.; KVEDARIENE, V.; ANNESI-MAESANO, I.; BLAIN, H.; BONNIAUD, P.; BOSSE, I.; DAUVILLIERS, Y.; DEVILLIER, P.; FONTAINE, J. F.; PEPIN, J. L.; PHAM-THI, N.; PORTEJOIE, F.; PICARD, R.; ROCHE, N.; ROLLAND, C.; SCHMIDT-GRENDELMEIER, P.; KUNA, P.; SAMOLINSKI, B.; ANTO, J. M.; CARDONA, V.; MULLOL, J.; PINNOCK, H.; RYAN, D.; SHEIKH, A.; WALKER, S.; WILLIAMS, S.; BECKER, S.; KLIMEK, L.; PFAAR, O.; BERGMANN, K. C.; MOSGES, R.; ZUBERBIER, T.; ROLLER-WIRNSBERGER, R. E.; TOMAZIC, P. V.; HAAHTELA, T.; SALIMAKI, J.; TOPPILA-SALMI, S.; VALOVIRTA, E.; VASANKARI, T.; GEMICIOGLU, B.; YORGANCIOGLU, A.; PAPADOPOULOS, N. G.; PROKOPAKIS, E. P.; TSILIGIANNI, I. G.; BOSNIC-ANTICEVICH, S.; O'HEHIR, R.; IVANCEVICH, J. C.; NEFFEN, H.; ZERNOTTI, M. E.; KULL, I.; MELEN, E.; WICKMAN, M.; BACHERT, C.; HELLINGS, P. W.; BRUSSELLE, G.; PALKONEN, S.; BINDSLEV-JENSEN, C.; ELLER, E.; WASERMAN, S.; BOULET, L. P.; BOUCHARD, J.; CHU, D. K.; SCHUNEMANN, H. J.; SOVA, M.; VRIES, G. De; EERD, M. van; AGACHE, I.; ANSOTEGUI, I. J.; BEWICK, M.; CASALE, T.; DYKEWICK, M.; EBISAWA, M.; MURRAY, R.; NACLERIO, R.; OKAMOTO, Y.; WALLACE, D. V.; BOUSQUET, J.; HELLINGS, P. W.; ABERER, W.; AGACHE, I.; AKDIS, C. A.; AKDIS, M.; ALIBERTI, M. R.; ALMEIDA, R.; AMAT, F.; ANGLES, R.; ANNESI-MAESANO, I.; ANSOTEGUI, I. J.; ANTO, J. M.; ARNAVIELLE, S.; ASAYAG, E.; ASARNOJ, A.; ARSHAD, H.; AVOLIO, F.; BACCI, E.; BACHERT, C.; BAIARDINI, I.; BARBARA, C.; BARBAGALLO, M.; BARONI, I.; BARRETO, B. A.; BASAGANA, X.; BATEMAN, E. D.; BEDOLLA-BARAJAS, M.; BEDBROOK, A.; BEWICK, M.; BEGHE, B.; BEL, E. H.; BERGMANN, K. C.; BENNOOR, K. S.; BENSON, M.; BERTORELLO, L.; BIALOSZEWSKI, A. Z.; BIEBER, T.; BIALEK, S.; BINDSLEV-JENSEN, C.; BJERMER, L.; BLAIN, H.; BLASI, F.; BLUA, A.; MARCINIAK, M. Bochenska; BOGUS-BUCZYNSKA, I.; BONER, A. L.; BONINI, M.; BONINI, S.; BOSNIC-ANTICEVICH, C. S.; BOSSE, I.; BOUCHARD, J.; BOULET, L. P.; BOURRET, R.; BOUSQUET, P. J.; BRAIDO, F.; BRIEDIS, V.; BRIGHTLING, C. E.; BROZEK, J.; BUCCA, C.; BUHL, R.; BUONAIUTO, R.; PANAITESCU, C.; CABANAS, M. T. Burguete; BURTE, E.; BUSH, A.; CABALLERO-FONSECA, F.; CAILLAUD, D.; CAIMMI, D.; CALDERON, M. A.; CAMARGOS, P. A. M.; CAMUZAT, T.; CANFORA, G.; CANONICA, G. W.; CARDONA, V.; CARLSEN, K. H.; CARREIRO-MARTINS, P.; CARRIAZO, A. M.; CARR, W.; CARTIER, C.; CASALE, T.; CASTELLANO, G.; CECCHI, L.; CEPEDA, A. M.; CHAVANNES, N. H.; CHEN, Y.; CHIRON, R.; CHIVATO, T.; CHKHARTISHVILI, E.; CHUCHALIN, A. G.; CHUNG, K. F.; CIARAVOLO, M. M.; CICERAN, A.; CINGI, C.; CIPRANDI, G.; COEHLO, A. C. Carvalho; COLAS, L.; COLGAN, E.; COLL, J.; CONFORTI, D.; SOUSA, J. Correia De; CORTES-GRIMALDO, R. M.; CORTI, F.; COSTA, E.; COSTA-DOMINGUEZ, M. C.; COURBIS, A. L.; COX, L.; CRESCENZO, M.; CRUZ, A. A.; CUSTOVIC, A.; CZARLEWSKI, W.; DAHLEN, S. E.; D'AMATO, G.; DARIO, C.; SILVA, J. Da; DAUVILLIERS, Y.; DARSOW, U.; BLAY, F. De; CARLO, G. De; DEDEU, T.; EMERSON, M. De Fatima; FEO, G. De; VRIES, G. De; MARTINO, B. De; RUBINA, N. P. Motta; DELEANU, D.; DEMOLY, P.; DENBURG, J. A.; DEVILLIER, P.; ERCOLANO, S. Di Capua; CARLUCCIO, N. Di; DIDIER, A.; DOKIC, D.; DOMINGUEZ-SILVA, M. G.; DOUAGUI, H.; DRAY, G.; DUBAKIENE, R.; DURHAM, S. R.; TOIT, G. Du; DYKEWICZ, M. S.; EL-GAMAL, Y.; EKLUND, P.; ELLER, E.; EMUZYTE, R.; FARRELL, J.; FARSI, A.; JR, J. Ferreira De Mello; FERRERO, J.; FINK-WAGNER, A.; FIOCCHI, A.; FOKKENS, W. J.; FONSECA, J. A.; FONTAINE, J. F.; FORTI, S.; FUENTES-PEREZ, J. M.; GALVEZ-ROMERO, J. L.; GAMKRELIDZE, A.; GARCIA-AYMERICH, J.; GARCA-COBAS, C. Y.; GARCIA-CRUZ, M. H.; GEMICIOGLU, B.; GENOVA, S.; CHRISTOFF, G.; GEREDA, J. E.; WIJK, R. Gerth Van; GOMEZ, R. M.; GOMEZ-VERA, J.; DIAZ, S. Gonzalez; GOTUA, M.; GRISLE, I.; GUIDACCI, M.; GULDEMOND, N. A.; GUTTER, Z.; GUZMAN, M. A.; HAAHTELA, T.; HAJJAM, J.; HERNANDEZ, L.; HOURIHANE, J. O' B.; HUERTA-VILLALOBOS, Y. R.; HUMBERT, M.; IACCARINO, G.; ILLARIO, M.; ISPAYEVA, Z.; IVANCEVICH, J. C.; JARES, E. J.; JASSEM, E.; JOHNSTON, S. L.; JOOS, G.; JUNG, K. S.; JUST, J.; JUTEL, M.; KAIDASHEV, I.; KALAYCI, O.; KALYONCU, A. F.; KARJALAINEN, J.; KARDAS, P.; KEIL, T.; KEITH, P. K.; KHAITOV, M.; KHALTAEV, N.; KLEINE-TEBBE, J.; KLIMEK, L.; KOWALSKI, M. L.; KUITUNEN, M.; KULL, I.; KUNA, P.; KUPCZYK, M.; KVEDARIENE, V.; KRZYCH-FALTA, E.; LACWIK, P.; LARENAS-LINNEMANN, D.; LAUNE, D.; LAURI, D.; LAVRUT, J.; LE, L. T. T.; LESSA, M.; LEVATO, G.; LI, J.; LIEBERMAN, P.; LIPIEC, A.; LIPWORTH, B.; CARLSEN, K. C. Lodrup; LOUIS, R.; LOURENCO, O.; LUNA-PECH, J. A.; MAGNAN, A.; MAHBOUB, B.; MAIER, D.; MAIR, A.; MAJER, I.; MALVA, J.; MANDAJIEVA, E.; MANNING, P.; KEENOY, E. De Manuel; MARSHALL, G. D.; MASJEDI, M. R.; MASPERO, J. F.; MATHIEU-DUPAS, E.; CAMPOS, J. J. Matta; MATOS, A. L.; MAURER, M.; MAVALE-MANUEL, S.; MAYORA, O.; MEDINA-AVALOS, M. A.; MELEN, E.; MELO-GOMES, E.; MELTZER, E. O.; MENDITTO, E.; MERCIER, J.; MICULINIC, N.; MIHALTAN, F.; MILENKOVIC, B.; MODA, G.; MOGICA-MARTINEZ, M. D.; MOHAMMAD, Y.; MOMAS, I.; MONTEFORT, S.; MONTI, R.; BOGADO, D. Mora; MORAIS-ALMEIDA, M.; MORATO-CASTRO, F. F.; MOSGES, R.; MOTA-PINTO, A.; SANTO, P. Moura; MULLOL, J.; MUNTER, L.; MURARO, A.; MURRAY, R.; NACLERIO, R.; NADIF, R.; NALIN, M.; NAPOLI, L.; NAMAZOVA-BARANOVA, L.; NEFFEN, H.; NIEDEBERGER, V.; NEKAM, K.; NEOU, A.; NIETO, A.; NOGUEIRA-SILVA, L.; NOGUES, M.; NOVELLINO, E.; NYEMBUE, T. D.; O'HEHIR, R. E.; ODZHAKOVA, C.; OHTA, K.; OKAMOTO, Y.; OKUBO, K.; ONORATO, G. L.; CISNEROS, M. Ortega; OUEDRAOGO, S.; PALI-SCHOLL, I.; PALKONEN, S.; PANZNER, P.; PAPADOPOULOS, N. G.; PARK, H. S.; PAPI, A.; PASSALACQUA, G.; PAULINO, E.; PAWANKAR, R.; PEDERSEN, S.; PEPIN, J. L.; PEREIRA, A. M.; PERSICO, M.; PFAAR, O.; PHILLIPS, J.; PICARD, R.; PIGEARIAS, B.; PIN, I.; PITSIOS, C.; PLAVEC, D.; POHL, W.; POPOV, T. A.; PORTEJOIE, F.; POTTER, P.; POZZI, A. C.; PRICE, D.; PROKOPAKIS, E. P.; PUY, R.; PUGIN, B.; ROSS, R. E. Pulido; PRZEMECKA, M.; RABE, K. F.; RACIBORSKI, F.; RAJABIAN-SODERLUND, R.; REITSMA, S.; RIBEIRINHO, I.; RIMMER, J.; RIVERO-YEVERINO, D.; RIZZO, J. A.; RIZZO, M. C.; ROBALO-CORDEIRO, C.; RODENAS, F.; RODO, X.; GONZALEZ, M. Rodriguez; RODRIGUEZ-MANAS, L.; ROLLAND, C.; VALLE, S. Rodrigues; RODRIGUEZ, M. Roman; ROMANO, A.; RODRIGUEZ-ZAGAL, E.; ROLLA, G.; ROLLER-WIRNSBERGER, R. E.; ROMANO, M.; ROSADO-PINTO, J.; ROSARIO, N.; ROTTEM, M.; RYAN, D.; SAGARA, H.; SALIMKI, J.; SAMOLINSKI, B.; SANCHEZ-BORGES, M.; SASTRE-DOMINGUEZ, J.; SCADDING, G. K.; SCHUNEMANN, H. J.; SCICHILONE, N.; SCHMID-GRENDELMEIER, P.; SERPA, F. S.; SHAMAI, S.; SHEIKH, A.; SIERRA, M.; SIMONS, F. E. R.; SIROUX, V.; SISUL, J. C.; SKRINDO, I.; SOLE, D.; SOMEKH, D.; SONDERMANN, M.; SOORONBAEV, T.; SOVA, M.; SORENSEN, M.; SORLINI, M.; SPRANGER, O.; STELLATO, C.; STELMACH, R.; STUKAS, R.; SUNYER, J.; STROZEK, J.; SZYLLING, A.; TEBYRICA, J. N.; THIBAUDON, M.; TO, T.; TODO-BOM, A.; TOMAZIC, P. V.; TOPPILA-SALMI, S.; TRAMA, U.; TRIGGIANI, M.; ULRIK, C. Suppli; URRUTIA-PEREIRA, M.; VALENTA, R.; VALERO, A.; VALIULIS, A.; VALOVIRTA, E.; EERD, M. Van; GANSE, E. Van; HAGUE, M. Van; VANDENPLAS, O.; VENTURA, M. T.; VEZZANI, G.; VASANKARI, T.; VATRELLA, A.; VERISSIMO, M. T.; VIART, F.; VIEGI, G.; VICHEVA, D.; VONTETSIANOS, T.; WAGENMANN, M.; WALKER, S.; WALLACE, D.; WANG, D. Y.; WASERMAN, S.; WERFEL, T.; WESTMAN, M.; WICKMAN, M.; WILLIAMS, D. M.; WILLIAMS, S.; WILSON, N.; WRIGHT, J.; WROCZYNSKI, P.; YAKOVLIEV, P.; YAWN, B. P.; YIALLOUROS, P. K.; YORGANCIOGLU, A.; YUSUF, O. M.; ZAR, H. J.; ZHANG, L.; ZHONG, N.; ZERNOTTI, M. E.; ZHANAT, I.; ZIDARN, M.; ZUBERBIER, T.; ZUBRINICH, C.; ZURKUHLEN, A.AimsMobile Airways Sentinel NetworK (MASK) belongs to the Fondation Partenariale MACVIA-LR of Montpellier, France and aims to provide an active and healthy life to rhinitis sufferers and to those with asthma multimorbidity across the life cycle, whatever their gender or socio-economic status, in order to reduce health and social inequities incurred by the disease and to improve the digital transformation of health and care. The ultimate goal is to change the management strategy in chronic diseases.MethodsMASK implements ICT technologies for individualized and predictive medicine to develop novel care pathways by a multi-disciplinary group centred around the patients.StakeholdersInclude patients, health care professionals (pharmacists and physicians), authorities, patient's associations, private and public sectors.ResultsMASK is deployed in 23 countries and 17 languages. 26,000 users have registered.EU grants (2018)MASK is participating in EU projects (POLLAR: impact of air POLLution in Asthma and Rhinitis, EIT Health, DigitalHealthEurope, Euriphi and Vigour).Lessons learnt(i) Adherence to treatment is the major problem of allergic disease, (ii) Self-management strategies should be considerably expanded (behavioural), (iii) Change management is essential in allergic diseases, (iv) Education strategies should be reconsidered using a patient-centred approach and (v) Lessons learnt for allergic diseases can be expanded to chronic diseases.
- Who has anaphylaxis in Brazil? Validation of a questionnaire for population studies(2017) GAGETE, Elaine; SANTOS, Lucilene Delazari dos; PONTES, Leticia Gomes de; CASTRO, Fabio MoratoBackground: The incidence of anaphylaxis is increasing in several parts of the world; thus, determining the prevalence of the disease in a given region is important to understand the factors involved and to promote measures to avoid this type of allergic reaction. Aiming this objective, we validated an instrument for a population-basedstudy that assesses the prevalence of anaphylaxis in the Brazilian population. Methods: A questionnaire was generated in two variants - one for subjects seven years old or above (Group A) and another for children who were up to six years, 11 months and 29 days (Group B). The instrument was administered to patients with and without anaphylaxis. By allocating points, a score was calculated to differentiate subjects with and without the disease. After validation, the questionnaire was applied in the city of Botucatu (Sao Paulo state, Brazil), by randomly selecting houses and inviting residents to answer the questionnaire. Results: The questionnaire was reliable for identifying subjects with and without anaphylaxis in both groups, with a specificity and sensitivity above 90%. The prevalence of anaphylaxis in the pilot survey was 6.2% in Group A, however the evaluation was compromised in Group B by the low number of children below seven years of age due to random sampling of residences. Discussion: The prevalence of anaphylaxis in our pilot test (6.2%) was similar to major epidemiological surveys from several parts of the world, showing that anaphylaxis is not a rare disease. The instrument of the present work was suitable for this epidemiological survey and might be a good option for studying anaphylaxis in other populations. Conclusion: This instrument might be of particular value in places where researchers cannot access medical records to conduct similar epidemiological studies.
- Fructose biphosphate aldolase: A new cassava allergen(2023) VENTURA, Anne K. R. M.; ALVES, Safiri de P.; CASTRO, Roberta A.; ROSSINI, Bruno C.; DELAZARI, Lucilense S.; OLIVEIRA, Amanda M. de; MORETTI, Ana I. S.; CASTRO, Fabio F. M.; KALIL, Jorge; YANG, Ariana C.; SANTOS, Keity S.Background: Food allergy has considerably increased in recent years and this situation has been aggravated mainly by the consumption of more processed and complex foods, since minor or potentially allergenic foods are not required to be labeled. Manihot esculenta (cassava) is a widely consumed food in South America, Africa, and Asia and can be used in the production of flour and starch, as well as several other products. This root can cause allergic reactions with symptoms ranging from mild to severe. Methods: Thus, the aim of this study was the characterization of the immunogenic cassava proteins responsible for sensitizing patients allergic to it. Using a 2D-SDS-PAGE based proteomic approach, six proteins were identified, including Fructose Bisphosphate Aldolase (FBA). Recom-binant FBA was produced in Expi293 cells and evaluated by immunoblotting with the serum of 10 individual study subjects. Results: Our results showed six cassava IgE-reactive proteins. From those, recombinant fructose bisphosphate aldolase (FBA) showed a positivity of 80% among tested sera, proving to be a highly sensitizing protein. Conclusion: The recombinant FBA molecule obtained in this study can be important for in vivo diagnostic assays, by producing more accurate results, and for desensitization protocols, in which the use of the isolated molecule produces more precise results by avoiding secondary sensitization. Trial registration: All patients signed a consent form approved by the internal ethics committee CAPPesq, Comissao de etica para Analise de Projetos de Pesquisa do HC FMUSP (CAAE: 10420619.6.0000.0068).