HERMES VIEIRA BARBEIRO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
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- Transcriptome analysis of six tissues obtained post-mortem from sepsis patients(2023) SILVA, Fabiano Pinheiro da; GONCALVES, Andre Nicolau Aquime; DUARTE-NETO, Amaro Nunes; DIAS, Thomaz Luescher; BARBEIRO, Hermes Vieira; BREDA, Cristiane Naffah Souza; BREDA, Leandro Carvalho Dantas; CAMARA, Niels Olsen Saraiva; NAKAYA, Helder I.Septic shock is a life-threatening clinical condition characterized by a robust immune inflammatory response to disseminated infection. Little is known about its impact on the transcriptome of distinct human tissues. To address this, we performed RNA sequencing of samples from the prefrontal cortex, hippocampus, heart, lung, kidney and colon of seven individuals who succumbed to sepsis and seven uninfected controls. We identified that the lungs and colon were the most affected organs. While gene activation dominated, strong inhibitory signals were also detected, particularly in the lungs. We found that septic shock is an extremely heterogeneous disease, not only when different individuals are investigated, but also when comparing different tissues of the same patient. However, several pathways, such as respiratory electron transport and other metabolic functions, revealed distinctive alterations, providing evidence that tissue specificity is a hallmark of sepsis. Strikingly, we found evident signals of accelerated ageing in our sepsis population.
- Chronic Low or High Nutrient Intake and Myokine Levels(2023) SIERRA, Ana Paula Renno; FONTES-JUNIOR, Antonio Alves; PAZ, Ines Assis; SOUSA, Cesar Augustus Zocoler de; MANOEL, Leticia Aparecida da Silva; MENEZES, Duane Cardoso de; ROCHA, Vinicius Alves; BARBEIRO, Hermes Vieira; SOUZA, Heraldo Possolo de; CURY-BOAVENTURA, Maria FernandaInadequate nutrient availability has been demonstrated to be one of the main factors related to endocrine and metabolic dysfunction. We investigated the role of inadequate nutrient intakes in the myokine levels of runners. Sixty-one amateur runners participated in this study. The myokine levels were determined using the Human Magnetic Bead Panel from plasma samples collected before and after the marathon. Dietary intake was determined using a prospective method of three food records. The runners with lower carbohydrate and calcium intakes had higher percentages of fat mass (p < 0.01). The runners with a sucrose intake comprising above 10% of their energy intake and an adequate sodium intake had higher levels of BDNF (p = 0.027 and p = 0.031). After the race and in the recovery period, the runners with adequate carbohydrate intakes (g/kg) (>5 g/kg/day) had higher levels of myostatin and musclin (p < 0.05). The runners with less than 45% of carbohydrate of EI had lower levels of IL-15 (p = 0.015) and BNDF (p = 0.013). The runners with higher cholesterol intakes had lower levels of irisin (p = 0.011) and apelin (p = 0.020), and those with a low fiber intake had lower levels of irisin (p = 0.005) and BDNF (p = 0.049). The inadequate intake influenced myokine levels, which promoted cardiometabolic tissue repair and adaptations to exercise.