OSMAR APARECIDO THEODORO JUNIOR
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Medicina Física e de Reabilitação, Hospital das Clínicas, Faculdade de Medicina
9 resultados
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conferenceObject Plant proteinase from Bauhinia bauhinioides Kallikrein inhibitor (BbKI) attenuates mechanics, inflammation and remodelling induced by elastase in mice(2012) OLIVEIRA, Bruno Martins; ALMEIDA-REIS, Rafael; THEODORO, Osmar A.; OLIVA, Leandro V.; RODRIGUES, Daniel Flisch; PINHEIRO, Nathalia; OLIVA, Maria L. V.; PRADO, Carla M.; MARTINS, Milton M.; TIBERIO, Iolanda F. L. C.- The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice(2016) MARTINS-OLIVERA, Bruno Tadeu; ALMEIDA-REIS, Rafael; THEODORO-JUNIOR, Osmar Aparecido; OLIVA, Leandro Vilela; NUNES, Natalia Neto dos Santos; OLIVO, Clarice Rosa; BRITO, Marlon Vilela de; PRADO, Carla Maximo; LEICK, Edna Aparecida; MARTINS, Milton de Arruda; OLIVA, Maria Luiza Vilela; RIGHETTI, Renato Fraga; TIBERIO, Iolanda de Fatima Lopes CalvoBackground. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2 alpha, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment.
conferenceObject Plant proteinase inhibitor from Enterolobium contortisiliquum (EcTI) attenuates elastase-induced pulmonary inflammatory, remodeling and mechanical alterations in mice(2012) THEODORO-JUNIOR, Osmar A.; MARQUES, Ricardo Henrique; ALMEIDA-REIS, Rafael; MARTINS-OLIVEIRA, Bruno T.; OLIVA, Leandro V.; LOPES, Tales; PRADO, Carla M.; OLIVA, Maria L. V.; MARTINS, Milton; TIBERIO, Iolanda F. L. C.conferenceObject Plant proteinase inhibitor from crataeva tapia (CrataBl) attenuates inflammation and remodelling induced by elastase in mice(2013) LEICK, Edna A.; OLIVA, Leandro Vilela; OLIVEIRA, Bruno Tadeu Martins; ALMEIDA-REIS, Rafael; THEODORO JR., Osmar A.; BRITO, Marlon Vilela de; PRADO, Carla Maximo; MARTINS, Milton A.; OLIVA, Maria Luiza Vilela; TIBERIO, Iolanda F. L. C.- A plant proteinase inhibitor from Crataeva tapia (CrataBL) attenuates elastase-induced pulmonary inflammatory, remodeling, and mechanical alterations in mice(2015) OLIVA, Leandro V.; ALMEIDA-REIS, Rafael; THEODORO-JUNIOR, Osmar; OLIVEIRA, Bruno M.; LEICK, Edna A.; PRADO, Carla M.; BRITO, Marlon V.; CORREIA, Maria Tereza dos Santos; PAIVA, Patricia M. G.; MARTINS, Milton A.; OLIVA, Maria Luiza V.; TIBERIO, Iolanda F. L. C.Chronic obstructive pulmonary disease (COPD) can lead to chronic and obstructive bronchitis and emphysema resulting in decreased bronchial lumen size. This study evaluated the effect of CrataBL, a protein isolated from the bark of Crataeva tapia, on lung mechanics, inflammation, and remodeling after elastase-induced pulmonary alterations in mice. The use of CrataBL led to decreased mechanical alterations, alveolar septum disruption (Lm), number of macrophage and neutrophil cells in the BALF, and TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS positive cells in the airways and alveolar walls compared to the animals in the ELA group. Moreover, a reduction in MUC-5-positive cells in the airway walls was observed. In conclusion, CrataBL attenuates changes in lung mechanics, inflammation, extracellular lung remodeling, and oxidative stress responses induced by the administration of elastase and decreased the volume fraction of isoprostane, collagen, and elastic fibers in the airways and alveolar walls compared to the animals in the ELA groups. Therefore, CrataBL is a potential therapeutic tool in the treatment of COPD.
conferenceObject Treatment with proteinase inhibitor, BbCI, modulates inflammatory response, mechanic alterations, and remodeling on elastase-induced emphysema in mice(2012) ALMEIDA-REIS, Rafael; OLIVEIRA, Bruno T. M.; THEODORO-JUNIOR, Osmar A.; OLIVA, Leandro V.; PINHEIRO, Nathalia; BRITO, Marlon V.; LOPES, Fernanda D. Q. T.; PRADO, Carla M.; MARTINS, Milton A.; OLIVA, Maria Luiza V.; TIBERIO, Iolanda F. L. C.conferenceObject Plant proteinase from bauhinia bauhinioides kallikrein inhibitor (BbKI) attenuates inflammation and remodelling induced by elastase in mice(2013) LEICK, Edna A.; OLIVEIRA, Bruno Tadeu Martins; ALMEIDA-REIS, Rafael; OLIVA, Leandro Vilela; THEODORO JR., Osmar A.; PRADO, Carla Maximo; MARTINS, Milton A.; OLIVA, Maria Luiza Vilela; TIBERIO, Iolanda F. L. C.conferenceObject Plant Kunitz proteinase inhibitor allenuates inflammation, oxidative stress and remodeling induced by elastase in mice(2014) LEICK, Edna K.; THEODORO-JUNIOR, Osmar A.; RIGHETTI, Renato F.; ALMEIDA-REIS, Rafael; OLIVA, Leandro; OLIVEIRA, Bruno T. Martins; PINHEIRO, Natalia M.; PRADO, Carla Maximo; OLIVA, Maria Luiza Vilela; MARTINS, Milton A.; TIBERIO, Iolanda F. L. C.bookPart Edema agudo de pulmão(2016) PINTO, Thiago Fernandes; THEODORO JUNIOR, Osmar