EDUARDO POMPEU
Projetos de Pesquisa
Unidades Organizacionais
SVAPRECLIN-05, Faculdade de Medicina
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
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conferenceObject Hyperacute Rejection in Multivisceral Xenotransplantation(2012) GALVAO, F.; SOLER, W.; POMPEU, E.; COSTA, A.; WAISBERG, D.; CAPELLOZI, V.; TEODORO, W.; VELOSA, A.; CHAIB, E.; D'ALBUQUERQUE, L.- Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage(2021) CRUZ, Isabele Camargo Brindo da; VELOSA, Ana Paula Pereira; CARRASCO, Solange; SANTOS FILHO, Antonio dos; MIRANDA, Jurandir Tomaz de; POMPEU, Eduardo; FERNANDES, Tiago Lazzaretti; BUENO, Daniela Franco; FANELLI, Camila; GOLDENSTEIN-SCHAINBERG, Claudia; FABRO, Alexandre Todorovic; FULLER, Ricardo; SILVA, Pedro Leme; CAPELOZZI, Vera Luiza; TEODORO, Walcy RosoliaCollagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes Col2a1 and Acan was significantly upregulated and Pou5fl was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, in vivo analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment.
- Immunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation(2012) GALVAO, Flavio H. F.; SOLER, Wangles; POMPEU, Eduardo; WAISBERG, Daniel R.; MELLO, Evandro S. D.; COSTA, Anderson C. L.; TEODORO, Walcy; VELOSA, Ana P.; CAPELOZZI, Vera L.; ANTONANGELO, Leila; CATANOZI, Sergio; MARTINS, Alessandro; MALBOUISSON, Luiz M. S.; CRUZ JR., Ruy J.; FIGUEIRA, Estela R.; FILHO, Joel A. R.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.Galvao FHF, Soler W, Pompeu E, Waisberg DR, Mello ES, Costa ACL, Teodoro W, Velosa AP, Capelozzi VL, Antonangelo L, Catanozi S, Martins A, Malbouisson LMS, Cruz RJ, Figueira ER, Filho JAR, Chaib E, D'Albuquerque LAC. Immunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation. Xenotransplantation 2012; 19: 298304. (c) 2012 John Wiley & Sons A/S. Abstract: Introduction: Xenotransplantation is a potential solution for the high mortality of patients on the waiting list for multivisceral transplantation; nevertheless, hyperacute rejection (HAR) hampers this practice and motivates innovative research. In this report, we describe a model of multivisceral xenotransplantation in which we observed immunoglobulin G (IgG) involvement in HAR. Methods: We recovered en bloc multivisceral grafts (distal esophagus, stomach, small intestine, colon, liver, pancreas, and kidneys) from rabbits (n = 20) and implanted them in the swine (n = 15) or rabbits (n = 5, control). Three hours after graft reperfusion, we collected samples from all graft organs for histological study and to assess IgG fixation by immunofluorescence. Histopathologic findings were graded according to previously described methods. Results: No histopathological features of rejection were seen in the rabbit allografts. In the swine-to-rabbit grafts, features of HAR were moderate in the liver and severe in esophagus, stomach, intestines, spleen, pancreas, and kidney. Xenograft vessels were the central target of HAR. The main lesions included edema, hemorrhage, thrombosis, myosites, fibrinoid degeneration, and necrosis. IgG deposition was intense on cell membranes, mainly in the vascular endothelium. Conclusions: Rabbit-to-swine multivisceral xenotransplants undergo moderate HAR in the liver and severe HAR in the other organs. Moderate HAR in the liver suggests a degree of resistance to the humoral immune response in this organ. Strong IgG fixation in cell membranes, including vascular endothelium, confirms HAR characterized by a primary humoral immune response. This model allows appraisal of HAR in multiple organs and investigation of the livers relative resistance to this immune response.
conferenceObject INCREASED COLLAGEN II EXPRESSION IN RABBIT MESENCHYMAL STEM CELLS STIMULATED BY COLLAGEN V: IS THERE A ROLE FOR CARTILAGE REPAIR?(2013) GOLDENSTEIN-SCHAINBERG, Claudia; VELOSA, Paula; CARRASCO, Solange; POMPEU, E.; FULLER, Ricardo; CAPELOZZI, Vera; CRUZ, Isabele; TEODORO, Walcy