RAUL CAVALCANTE MARANHAO
Projetos de Pesquisa
Unidades Organizacionais
FBC, FCF - Docente
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/31 - Laboratório de Genética e Hematologia Molecular, Hospital das Clínicas, Faculdade de Medicina
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/31 - Laboratório de Genética e Hematologia Molecular, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Association Between Lipid Transfer To HDL-c And Arterial Stiffness In Patients With Ischemic Stroke Due To Hypertensive Emergency(2021) OLIVEIRA-ANDRADE, Days; FREITAS, Fatima; AGUIAR, Franciana; MANSOR, Ana Luiza; COSENSO-MARTIN, Luciana; SOUZA, Doroteia R.; MARANHAO, Raul C.; VILELA-MARTIN, Jose F.
- L-Glutamine supplementation enhances glutathione peroxidase and paraoxonase-1 activities in HDL of exercising older individuals(2021) PIRES, Renier S.; BRAGA, Pedro G. S.; SANTOS, Juliana M. B.; AMARAL, Jonatas B.; AMIRATO, Gislene R.; TRETTEL, Caio S.; SANTOS, Carlos A. F. Dos; VAISBERG, Mauro; NALI, Luis H. S.; VIEIRA, Rodolfo P.; MARANHAO, Raul C.; PITHON-CURI, Tania C.; BARROS, Marcelo P.; BACHI, Andre L. L.Background: Oxidative stress is an important factor in the formation of atherosclerotic plaques. High-density lipoprotein (HDL) harbors paraoxonase-1 (PON-1) and glutathione peroxidase (GPx), key enzymes in the protection against the harmful effects of oxidative stress. Although exercise training can increase both HDL-c content and its antioxidant action, and glutamine (Gln) intake also promotes GPx-based defenses, the association between exercise training and Gln in the regulation of PON-1 activity was not explored. Therefore, the objective of this study was to investigate the effects of Gln supplementation on the redox balance and on the total HDL antioxidant capacity by evaluation of the activity of PON-1 and GPx enzymes in physically exercised elderly individuals compared to non-exercised ones. Methods: Fifty-one practitioners of a combined exercise training program (CET, age: 71.9 +/- 5.7 years) and 32 non-practitioners (NP, age: 73 +/- 6.3 years) participated in the study. CET and NP groups were separated into 2 subgroups according to the supplementation: Gln, 0.3 g/kg/day + 10 g maltodextrin (CET-Gln, n = 26; and NP-Gln, n = 16) or placebo, 10 g maltodextrin (CET-PL, n = 25; and NP-PL, n = 16). Blood samples were drawn at baseline and after 30 days after commencement of the supplementation for biochemical and enzyme activity analyses. Results: Increased HDL-c, total peroxidase (PRx), and GPx activities were found in both CET-Gln and NP-Gln after the supplementation period, compared to baseline, in opposition to CET-PL and NP-PL groups. PON-1 activity increased only in CET-Gln. In both CET-Gln and NP-Gln groups, there was a reduction of the total peroxides/PRx, iron/PRx, and total peroxides/GPX ratios after supplementation. In CET-Gln, thiobarbituric acid-reactive substances (TBARS)/PRx and TBARS/GPx ratios were also lower after supplementation. CET-Gln and CET-PL subgroups had lower glycemia than NP-Gln and NP-PL, either at baseline or after the supplementation periods. The other parameters were unchanged after supplementation [total cholesterol, LDL-c, triglycerides, non-HDL cholesterol, total peroxides, TBARS, iron serum, Trolox-equivalent antioxidant capacity (TEAC), and uric acid]. Conclusions: Gln supplementation can increase glutathione peroxidase activity regardless the individuals were physically active or sedentary, but the PON-1 activity only increased in physically active individuals. These results show the potential of Gln supplementation in the maintenance of the vascular redox balance, with potential implications for atherogenesis protection.
- Use of paclitaxel carried in lipid nanoparticles to treat aortic allograft transplantation in rats(2021) PEPINELI, Rafael; SANTANA, Alexandre C.; SILVA, Filipe M. O.; TAVONI, Thauany M.; STOLF, Noedir A. G.; NORONHA, Irene L.; MARANHAO, Raul C.Objectives The aim of this study was to test whether lipid core nanoparticles loaded with paclitaxel (LDE-PTX) protect rat aortic allograft from immunological damage. Methods Fisher and Lewis rats were used differing in minor histocompatibility loci. Sixteen Lewis rats were allocated to four-animal groups: SYNG (syngeneic), Lewis rats receiving aorta grafts from Lewis rats; ALLO (allogeneic), Lewis rats receiving aortas from Fisher rats; ALLO+LDE (allogeneic transplant treated with LDE), Lewis rats receiving aortas from Fisher rats, treated with LDE (weekly injection for 3 weeks); ALLO+LDE-PTX (allogeneic transplant treated with LDE-PTX), Lewis rats receiving aortas from Fisher rats treated with LDE-PTX (4 mg/kg weekly for 3 weeks). Treatments began on transplantation day. Results Thirty days post-transplantation, SYNG showed intact aortas. ALLO and ALLO+LDE presented intense neointimal formation. In ALLO+LDE-PTX, treatment inhibited neointimal formation; narrowing of aortic lumen was prevented in ALLO and ALLO+LDE. LDE-PTX strongly inhibited proliferation and intimal invasion by smooth muscle cells, diminished 4-fold presence of apoptotic/dead cells in the intima, reduced the invasion of aorta by macrophages and T-cells and gene expression of pro-inflammatory tumour necrosis factor-alpha (TNF alpha), interferon gamma (IFN gamma) and interleukin-1 beta (IL-1 beta). Conclusions LDE-PTX was effective in preventing the vasculopathy associated with rejection and may offer a potent therapeutic tool for post-transplantation.
- Chlorine, chromium, proteins of oxidative stress and DNA repair pathways are related to prognosis in oral cancer(2021) ASSIS, Aricia Leone Evangelista Monteiro de; ARCHANJO, Anderson Barros; MARANHAO, Raul C.; MENDES, Suzanny O.; SOUZA, Rafael P. de; CICCO, Rafael de; OLIVEIRA, Mayara M. de; BORCOI, Aline R.; MAIA, Lucas de L.; NUNES, Fabio D.; SANTOS, Marcelo dos; TRIVILIN, Leonardo O.; PINHEIRO, Christiano J. G.; ALVARES-DA-SILVA, Adriana M.; NOGUEIRA, Breno ValentimThe comparison of chemical and histopathological data obtained from the analysis of excised tumor fragments oral squamous cell carcinoma (OSCC) with the demographic and clinical evolution data is an effective strategy scarcely explored in OSCC studies. The aim was to analyze OSCC tissues for protein expression of enzymes related to oxidative stress and DNA repair and trace elements as candidates as markers of tumor aggressiveness and prognosis. Tumor fragments from 78 OSCC patients that had undergone ablative surgery were qualitatively analyzed by synchrotron micro-X-ray fluorescence for trace elements. Protein expression of SOD-1, Trx, Ref-1 and OGG1/2 was performed by immunohistochemistry. Sociodemographic, clinical, and histopathological data were obtained from 4-year follow-up records. Disease relapse was highest in patients with the presence of chlorine and chromium and lowest in those with tumors with high OGG1/2 expression. High expression of SOD-1, Trx, and Ref-1 was determinant of the larger tumor. Presence of trace elements can be markers of disease prognosis. High expression of enzymes related to oxidative stress or to DNA repair can be either harmful by stimulating tumor growth or beneficial by diminishing relapse rates. Interference on these players may bring novel strategies for the therapeutic management of OSCC patients.
conferenceObject HIGH-DENSITY LIPOPROTEIN FUNCTION IN PATIENTS WITH TYPE 2 DIABETES: DIFFERENCES BETWEEN THE ACUTE AND CHRONIC PHASES OF CORONARY ARTERY DISEASE(2021) GENESTRETI, Paulo Rizzo; FREITAS, Fatima R.; FURTADO, Remo; RODRIGUEZ, Rocio Salsoso; DALCOQUIO, Talia; TAVONI, Thauany; NAKASHIMA, Carlos; FERRARI, Aline Gehlen; COLODETTI, Raiza; MENEZES, Fernando; LIMA, Viviane; BARACIOLI, Luciano; MARANHAO, Raul C.; NICOLAU, Jose- Disturbances of the transfer of cholesterol to high-density lipoprotein (HDL) in patients with peripheral artery disease with or without type 2 diabetes mellitus(2021) RIBEIRO, Flavio Roberto Cavalleiro de Macedo; RIBEIRO, Carolina Heitmann Mares Azevedo; TAVONI, Thauany Martins; SARGES, Erica dos Santos; FREITAS, Fatima Rodrigues; STOLF, Noedir Antonio Groppo; KALIL FILHO, Roberto; MARANHAO, Raul CavalcanteIntroduction: Low high-density lipoprotein (HDL)-cholesterol is frequent in patients with peripheral artery disease (PAD) and also in type 2 diabetes mellitus (T2DM), the major risk factor for PAD. The transfer of cholesterol from the other lipoproteins to HDL is an important aspect of HDL metabolism and function, and may contribute to atherogenic mechanisms that lead to PAD development. Objective: The aim of this study was to investigate the status of cholesterol transfers in patients with PAD without or with T2DM. Methods: Patients with PAD (n = 19), with PAD and T2DM (PAD + DM, n = 19), and healthy controls (n = 20), all paired for age, gender, and BMI were studied. Transfer of both forms of cholesterol, unesterified (UC) and esterified (EC), was performed by incubating plasma with a donor nanoemulsion containing radioactive UC and EC, followed by chemical precipitation and HDL radioactive counting. Results: Low-density lipoprotein (LDL)-cholesterol and triglycerides were similar in the three groups. Compared to controls, HDL-C was lower in PAD + DM (p < 0.05), but not in PAD. Transfer of UC was lower in PAD + DM than in PAD and controls (4.18 +/- 1.17%, 5.13 +/- 1.44%, 6.59 +/- 1.25%, respectively, p < 0.001). EC transfer tended to be lower in PAD + DM than in controls (2.96 +/- 0.60 vs 4.12 +/- 0.89%, p = 0.05). Concentrations of cholesteryl ester transfer protein (CETP) and lecithin-cholesterol acyltransferase (LCAT), both involved in HDL metabolism, were not different among the three groups. Conclusion: Deficient cholesterol transfer to HDL may play a role in PAD pathogenesis. Since UC transfer to HDL was lower in PAD + DM compared to PAD alone, it is possible that defective HDL metabolism may contribute to the higher PAD incidence in patients with T2DM.Keywords
conferenceObject CHOLESTEROL TRANSFER TO HDL IN HEALTHY ELDERLY AND YOUNG INDIVIDUALS: FAVORABLE EFFECTS OF CARDIORESPIRATORY FITNESS IN PROTECTION AGAINST ATHEROSCLEROSIS.(2021) BRAGA, P. G. S.; TAVONI, T. M.; BARONI, R. V.; ALVES, M. J. N. N.; ROCHA, G. A.; BACHI, A. L. L.; NEGRAO, C. E.; VAISBERG, M. W.; MARANHAO, R. C.