LUCIA MARIA ALMEIDA BRAZ
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Medicina Preventiva, Faculdade de Medicina
LIM/38 - Laboratório de Epidemiologia e Imunobiologia, Hospital das Clínicas, Faculdade de Medicina
LIM/38 - Laboratório de Epidemiologia e Imunobiologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- EVALUATION OF THE "SYMBIOSYS" IMMUNOASSAY FOR THE SEROLOGICAL DIAGNOSIS OF CHAGAS DISEASE(2012) SOUZA, Regina Maia de; AMATO NETO, Vicente; BEZERRA, Rita Cristina; GAKYIA, Erika; BRAZ, Lucia Maria Almeida
- USEFULNESS OF kDNA PCR IN THE DIAGNOSIS OF VISCERAL LEISHMANIASIS REACTIVATION IN CO-INFECTED PATIENTS(2013) NICODEMO, Antonio Carlos; AMATO, Valdir Sabbaga; TUON, Felipe Francisco; SOUZA, Regina Maia de; OKAY, Thelma Suely; ALMEIDA, Lucia MariaIt is important to develop new methods for diagnosing relapses in the co-infection of visceral leishmaniasis (VL) and HIV to enable earlier detection using less invasive methods. We report a case of a co-infected patient who had relapses after VL treatment, where the qualitative kDNA PCR showed a good performance. The kDNA PCR seems to be a useful tool for diagnosing VL and may be a good marker for predicting VL relapses after treatment of co-infected patients with clinical symptoms of the disease.
bookPart Protozooses intestinais(2013) GAKIYA, Erika; BRAZ, Lúcia Maria Almeida; SOUZA, Regina Maia de; BEZERRA, Rita Cristina; AMATO NETO, Ruth Semira Rodríguez Alarcónarticle 4 Citação(ões) na Scopus Unusual Clinical Manifestations of Leishmania (L.) infantum chagasi in an HIV-coinfected Patient and the Relevance of ITS1-PCR-RFLP: A Case Report(2018) DE, De Godoy Natalia Souza; DEMARCHI, Aiello Vera; MAIA, De Souza Regina; THELMA, Okay; ALMEIDA, Braz Lucia MariaPatients coinfected with Leishmania/HIV can develop atypical forms of visceral leishmaniasis (VL), making it indispensable to identify the etiological agent. We are presenting a postmortemspecie definition by ITS1-PCR-RFLP in a larynx tissue of a patient presented coinfection Leishmania/HIV. This patient was from a leishmaniasis endemic region in Sao Paulo(SP), Brazil, and was diagnosed clinically with mucocutaneous leishmaniasis. Before a rK39 immunochromatographic test positive, a tiny stored paraffin-embedded larynx tissue wasobtained post-mortem and submitted to 3 conventional PCR assays: kDNA (K20/K22 and RV1/RV2), and ITS1 (LITSR/L5.8S). The last one was followed by RFLP (HaeIII) and analyzed by 4% Metaphor agarose gel electrophoresis. Leishmania genus and Leishmania (Leishmania) subgenus were defined by kDNA-PCR, with K20/K22 (120 bp) and RV1/RV2 (145 bp), respectively. ITS1-PCR-RFLP identified L. (L.) infantum chagasi species visualized by the restriction patterns of 180, 70 and 50 bp. This case draws attention to the necessity for a clear identification of the etiological agent causing infection, especially in endemicregions of cutaneous and visceral leishmaniasis, and particularly in patients with comorbidities who often present atypical forms of the disease. L. (L.) infantum chagasi, which is usually responsible for VL, had changed its clinical spectrum for mucocutaneous. Unequivocal identification was carried out by ITS-PCR-RFLP, therefore confirming rK39 result. These techniques, which complemented each other, have a convenient cost-benefit ratio that makes them suitable to be applied in developing countries.bookPart Leishmanaiose Visceral(2016) AMATO, Valdir Sabbaga; SOUZA, Regina Maia de; BRAZ, Lucia Maria Almeida; CAMARGO, Raphael Abegão de; TUON, Felipe Francisco Bondan; NICODEMO, Elisabeth Lima- Reactivation of cutaneous and mucocutaneous tegumentary leishmaniasis in rheumatoid arthritis patients: an emerging problem?(2017) SOUZA, Regina Maia de; ANDRADE JUNIOR, Heitor Franco de; DUARTE, Maria Irma Seixas; BRAZ, Lucia Maria Almeida; SCHUBACH, Armando de Oliveira; SILVA, Fatima Conceicao; AMATO, Valdir SabbagaRheumatoid arthritis (RA) is a chronic condition that is frequent in patients living in tropical areas exposed to leishmaniasis. RA therapy involves immunosuppressant drugs such as methotrexate (MTX), monoclonal antibodies (mAbs) and prednisone. We report an unusual presentation of cutaneous (CL) or mucocutaneous leishmaniasis (ML) in RA patients from an endemic area of leishmaniasis. A 51-year-old woman noted a cutaneous ulcer on her left ankle during MTX and prednisone RA therapy. Initially diagnosed as a venous stasis ulcer, the aspirate of the injury revealed the presence of Leishmania DNA. A 73-year-old woman presenting non-ulcerated, infiltrated and painful erythematous nodules inside her nostrils while receiving MTX, anti-TNF mAb, and prednisone for RA, had also the aspirate of injuries showing the presence of Leishmania DNA. Both patients healed after the therapy with liposomal amphotericin. The RA therapy has changed to low-dose prednisone, without further reactivation episodes. Both cases suggest that CL or ML can reactivate after administration of an immunosuppressant for RA treatment. Therefore, immunosuppressive treatments for RA should be carefully prescribed in patients from endemic areas or with a history of CL and ML.
- Influence of chitosan/clay in drug delivery of glucantime from PVP membranes(2014) OLIVEIRA, Maria J. A.; SILVA, Estefania O.; BRAZ, Lucia M. A.; MAIA, Regina; AMATO, Valdir S.; LUGAO, Ademar B.; PARRA, Duclerc F.Polymeric hydrogels are receiving much attention in the past few years, as intelligent materials due to their properties for biomaterials. In this work, hydrogels of poly(N-2-vinil-pirrolidone) (PVP) containing chitosan and clay nanoparticles were obtained and characterized for glucantime drug delivery. The matrixes were crosslinked by gamma irradiation process with doses of 25 kGy. Hydrogels morphologies were observed by a Scanning Electron Microscope (SEM). The structural properties of the network were determined by gel fraction and swelling kinetic at 22 degrees C to study the capacity of water retention and, finally, drug delivery tests were performed ""in vitro"". The system showed higher gel fraction for the matrix with 1.0% of clay. In this case, besides the interactions of clay ions with PVP, there were interactions of chitosan with PVP. The results of glucantime delivery at the chitosan/PVP and clay system were discussed.