FLAVIO AUGUSTO DE PADUA MILAGRES
Projetos de Pesquisa
Unidades Organizacionais
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Detection of coinfection with Chikungunya virus and Dengue virus serotype 2 in serum samples of patients in State of Tocantins, Brazil(2020) MARINHO, Robson dos Santos S.; DURO, Rodrigo L. Sanz; SANTOS, Giulia L.; HUNTER, James; TELES, Maria da Aparecida Rodrigues; BRUSTULIN, Rafael; MILAGRES, Flavio A. de Padua; SABINO, Ester C.; DIAZ, Ricardo S.; V, Shirley KomninakisBackground: The co-circulation of Chikungunya (CHIKV), Dengue (DENV) and Zika (ZIKV) viruses increased the risk of outbreaks and coinfections among them. Here, we report cases of coinfection in clinical samples from state of Tocantins, Brazil. Methods: In 2017, the Central Public Health Laboratory (LACEN) received samples of patients who consulted health units with symptoms compatible with arboviral infections. A total of 102 samples were sent to the Retrovirology Laboratory at the Federal University of Sao Paulo, where they were tested by RT-qPCR to confirm DENV, ZIKV and CHIKV infections and to detect coinfected patients. Results: We identified with CHIKV monoinfection (52), DENV serotypes 1 (28) and serotypes 2 (22). We did not detect ZIKV. Five patients were characterized with coinfection involving CHIKV and DENV serotype 2. Conclusions: The presence of co-circulating arboviruses increases the chance of coinfection and demonstrates the importance of differential diagnosis and vector control. (C) 2020 Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences.
- Wuhan large pig roundworm virus identified in human feces in Brazil(2018) LUCHS, Adriana; LEAL, Elcio; KOMNINAKIS, Shirley Vasconcelos; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; GILL, Danielle Elise; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys daWe report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.
- Complete Genome Sequences of Six Human Bocavirus Strains from Patients with Acute Gastroenteritis in the North Region of Brazil(2018) WATANABE, Aripuana Sakurada Aranha; LUCHS, Adriana; LEAL, Elcio; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; GILL, Danielle Elise; LOBATO, Marcia Cristina Alves Brito Sayao; BRUSTULIN, Rafael; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; COSTA, Antonio Charlys daHuman bocavirus (HBoV) is commonly associated with acute respiratory tract illness and gastroenteritis. We report six complete genomic sequences of HBoV strains from patients with gastroenteritis in Belem do Para and Tocantins in the North Region of Brazil. Phylogenetic analysis indicated that the six HBoV strains belong to genotypes 1, 2, and 3.
- Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil(2020) TAHMASEBI, Roozbeh; COSTA, Antonio Charlys da; TARDY, Kaelan; TINKER, Rory J.; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; CHAGAS, Rogerio Togisaki das; SOARES, Cassia Vitoria de Deus Alves; WATANABE, Aripuana Sakurada Aranha; ALENCAR, Cecilia Salete; VILLANOVA, Fabiola; DENG, Xutao; DELWART, Eric; LUCHS, Adriana; LEAL, Elcio; SABINO, Ester CerdeiraHuman Adenovirus species C (HAdV-C) is the most common etiologic agent of respiratory disease. In the present study, we characterized the nearly full-length genome of one potential new HAdV-C recombinant strain constituted by Penton and Fiber proteins belonging to type 89 and a chimeric Hexon protein of types 1 and 89. By using viral metagenomics techniques, we screened out, in the states of Tocantins and Para, Northern and North regions of Brazil, from 2010 to 2016, 251 fecal samples of children between 0.5 to 2.5 years old. These children were presenting acute diarrhea not associated with common pathogens (i.e., rotavirus, norovirus). We identified two HAdV-C strains in two distinct patients. Phylogenetic analysis performed using all complete genomes available at GenBank database indicated that one strain (HAdV-C BR-245) belonged to type 1. The phylogenetic analysis also indicated that the second strain (HAdV-C BR-211) was located at the base of the clade formed by the newly HAdV-C strains type 89. Recombination analysis revealed that strain HAdV-C BR-211 is a chimera in which the variable regions of Hexon gene combined HAdV-C1 and HAdV-C89 sequences. Therefore, HAdV-C BR-211 strain possesses a genomic backbone of type HAdV-C89 and a unique insertion of HAdV-C1 in the Hexon sequence. Recombination may play an important driving force in HAdV-C diversity and evolution. Studies employing complete genomic sequencing on circulating HAdV-C strains in Brazil are needed to understand the clinical significance of the presented data.
- Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus(2018) COSTA, Antonio Charlys da; LUCHS, Adriana; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; GILL, Danielle Elise; LOBATO, Marcia Cristina Alves Brito Sayao; BRUSTULIN, Rafael; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; LEAL, ElcioHere we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.
- Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil(2018) COSTA, Antonio Charlys da; LUCHS, Adriana; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; GILL, Danielle Elise; LOBATO, Marcia Cristina Alves Brito Sayao; BRUSTULIN, Rafael; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; LEAL, ElcioIn the present article we report the nearly full length genome of a Cosavirus strain (BRTO-83) isolated from a child with acute gastroenteritis, and who is an inhabitant of a rural area in the central region of Brazil. The sample was previously screened and negative for both: common enteric viruses (i.e. rotavirus and norovirus), bacteria, endoparasites and helminthes. Evolutionary analysis and phylogenetic inferences indicated that the Brazilian BRTO-83 Cosavirus strain was a recombinant virus highly related to the E/D recombinant NG385 strain (Genbank JN867757), which was isolated in Nigeria from an acute flaccid paralysis patient. This is the first report of a recombinant E/D Cosavirus strain detected in Brazil, and the second genome described worldwide. Further surveillance and molecular studies are required to fully understand the epidemiology, distribution and evolution of the Cosavirus.
- Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil(2019) LEAL, Elcio; LUCHS, Adriana; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; GILL, Danielle Elise; LOBATO, Marcia Cristina Alves Brito Sayao; BRUSTULIN, Rafael; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; VILLANOVA, Fabiola; WITKIN, Steven S.; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; COSTA, Antonio Charlys daWe characterized the 24 nearly full-length genomes of human parechoviruses (PeV) from children in the north of Brazil. The initial phylogenetic analysis indicated that 17 strains belonged to genotype 1, 5 to genotype 4, and 1 to genotype 17. A more detailed analysis revealed a high frequency of recombinant strains (58%): A total of 14 of our PeV-As were chimeric, with four distinct recombination patterns identified. Five strains were composed of genotypes 1 and 5 (Rec1/5); five strains shared a complex mosaic pattern formed by genotypes 4, 5, and 17 (Rec4/17/5); two strains were composed of genotypes 1 and 17 (Rec1/17); and two strains were composed of genotype 1 and an undetermined strain (Rec1/und). Coalescent analysis based on the Vp1 gene, which is free of recombination, indicated that the recombinant strains most likely arose in this region approximately 30 years ago. They are present in high frequencies and are circulating in different small and isolated cities in the state of Tocantins. Further studies will be needed to establish whether the detected recombinant strains have been replacing parental strains or if they are co-circulating in distinct frequencies in Tocantins.