CLARISSE MARTINS MACHADO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
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conferenceObject Use of Adhesive Tags as a Facilitating Tool in the Data Report of Chronic Gvhd in a HSCT Center Lacking Electronic Medical Records(2018) SILVA, Paula Moreira da; SIMIONE, Anderson Joao; SONA, Bruna Fernanda; COLTURATO, Lag; MAUAD, Lenira Maria Queiroz; SANTOS, Ana Claudia Ferrari dos; MACHADO, Clarisse Martins; POSSANI, Valquiria de Cassia; FRANCESCHI, Fernanda Leite de Souza; SABAINI, Carlos Sitta; COLTURATO, Vergilio A. R.; SOUZA, Mair Pedro De- Thymopoiesis in Pre- and Post-Hematopoietic Stem Cell Transplantation(2018) ROCHA, Luis Klaus A. da; BARROS, Samar Freschi de; BANDEIRA, Francine; BOLLINI, Alexia; TESTA, Lucia Helena de A.; SIMIONE, Anderson Joao; SOUZA, Marina de O. e; ZANETTI, Lilian P.; OLIVEIRA, Leila Cibele S. de; SANTOS, Ana Claudia F. dos; SOUZA, Mair Pedro de; COLTURADO, Vergilio Antonio R.; KALIL, Jorge; MACHADO, Clarisse M.; GUILHERME, LuizaHematopoietic stem cell transplantation (HSCT) is an important therapeutic option for some hematological diseases. However, patients who undergo HSCT acquire a state of immunodeficiency that causes significant mortality. Reconstitution of thymic function is needed to support the immune system. One way to measure thymic function is through T-cell receptor excision circle (TREC) quantification. TRECs are generated by T-cell receptor gene rearrangements during T-cell maturation in the thymus and represent a reliable marker for thymic output. In this study, we aimed to assess aging and malignant hematological diseases as two important factors that may influence thymic output before HSCT. We observed that patients before HSCT presented signal joint TREC (sjTREC) numbers lower than 606.55 copies/mu g DNA (low values) compared with healthy individuals, with an odds ratio (OR) of 12.88 [95% confidence interval (CI): 5.26-31.53; p < 0.001]. Our results showed that a group of older individuals (>= 50 years old), comprising both healthy individuals and patients, had an OR of 10.07 (95% CI: 2.80-36.20) for low sjTREC values compared with younger individuals (<= 24 years old; p < 0.001). Multiple logistic regression analysis confirmed that both older age (>= 50 years old) and malignant hematological diseases and their treatments were important and independent risk factors related to thymic function impairment (p < 0.001). The median sjTREC value for patients of all ages was significantly lower than the sjTREC median for the subgroup of older healthy individuals (>= 50 years old; p < 0.001). These data suggested that patients before HSCT and healthy individuals exhibited age-dependent thymic impairment, and that prior treatment for hematological diseases may exacerbate aging-related deterioration of natural thymic function. Furthermore, we analyzed these patients 9 months post-HSCT and compared patients who underwent autologous HSCT with those who underwent allogeneic HSCT. Both groups of patients achieved sjTREC copy numbers similar to those of healthy individuals. We did not find a close relationship between impaired thymic function prior to HSCT and worse thymic recovery after HSCT.
conferenceObject Revisiting viral surveillance in haploidentical stem cell transplantation (haploSCT). A case-control study(2018) COLTURATO, Iago; MORENO, Juliana P.; ZANETTI, Lilian P.; OLIVEIRA, Leila C. S. de; FRANCESCHI, Fernanda S.; SABAINI, Carlos S.; SANTOS, Ana Claudia F. dos; SILVA, Paula M. da; SOUZA, Mair P. de; COLTURATO, Vergilio R.; MACHADO, Clarisse M.- Chikungunya, Dengue, and Zika in Immunocompromised Hosts(2018) DARRIGO JR., Luiz Guilherme; CARVALHO, Alexandre Machado de Sant'Anna; MACHADO, Clarisse MartinsPurpose of Review Describe the characteristics of chikungunya, dengue, and Zika in transplant recipients and immunocompromised hosts. Recent Findings Stem cell/bone marrow grafts, organs, and blood transfusions can transmit CHIKV/DENV/ZIKV infections, which are clinically similar, resembling influenza-like illness. Laboratory confirmation is necessary. In the acute phase, RT-PCR is preferred. DENV and ZIKV serology may cross-react. Delayed engraftment and extended viruria is observed in ZIKV+/HSCT recipients, while longer viremia is observed in DENV+/HSCT patients. Arbovirus persistence in organ tissues is generally unknown. Vaccine development is in early stages for CHIKV/ZIKV. No data is available to recommend the licensed DENV vaccine in transplant recipients. Summary In endemic areas, the assessment of epidemiological risk is mandatory. Donor deferral for 120 days in suspected or confirmed ZIKV+ has been recommended, while CHIKV+ donors should wait 30 days. No deferral is recommended for DENV+ donors. CHIKV/DENV/ZIKV tests should be included in the differential of febrile neutropenia and other transplant syndromes. Reassessment of DENV serology is urgently needed. Prospective studies are necessary to determine the impact of CHIKV/DENV/ZIKV in this special population.
- Cut-Off of COBAS (R) Ampliprep/COBAS (R) Taqman (R) CMV Test in Transplant Recipients Receiving Intermittent Ganciclovir (GCV) Prophylaxis(2018) CAMPOS, Silvia Vidal; SOUZA, Ana Carolina M.; MELLO, Liliane S.; PEREIRA, Barbara B. S.; MACHADO, Clarisse M.
conferenceObject Influenza A and B infection are clinically similar in HSCT recipients(2018) SABAINI, Carlos S.; SIMINONE, Anderson J.; SILVA, Paula M. da; ZANETTI, Lilian P.; OLIVEIRA, Leila C. S. de; SOUZA, Marina de Oliveira e; MORENO, Juliana P.; FRANCESCHI, Fernanda S.; COLTURATO, Iago; SOUZA, Mair P.; COLTURATO, Vergilio R.; MACHADO, Clarisse M.